FastQCFastQC Report
Wed 25 May 2016
SRR2088283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2414591
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT334651.3859490075130738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT308471.2775248478934942No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT264651.0960448374072462No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG156460.647977235067968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116470.4823591241746532No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT96000.39758286185942054No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA89950.3725268585859883No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT89000.36859244484883774No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA88410.36614896684366005No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA71490.2960749874409372No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT67160.2781423437758196No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG65780.27242709013659044No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT64120.26555221981693794No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53980.22355753003303666No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA53920.2233090407443745No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT45810.18972157189354222No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44310.18350933967698876No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA40770.16884847164592265No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.16855856747581682No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC37350.1546845821921808No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34200.14163889453741857No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30880.1278891538981136No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC30860.12780632413522622No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30310.12552850565582327No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.12287795324342715No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29550.12238097466610287No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26560.10999792511443968No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC25650.1062291709030639No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC24970.10341295896489301No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC24300.10063816190816581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75200.030.4561181
ATTGGAC7550.021.5629123
GTATTGG7350.020.8911551
TATTGGA8850.019.2316382
GTATCAA129900.018.2151662
GGACCCT14250.018.0456146
TTGGACC14700.017.8707494
GTTCGCG650.00158061517.07692337
TGGACCC15550.016.6559475
TCGACGA803.3844952E-416.187537
GACCCTC16300.015.5490797
ACGGACC1201.9375511E-615.4166668
CTAGTAC2650.015.3584913
AGTATCA4600.014.8804351
TTTTTAC16250.014.6861531
CGAATTA1304.448553E-614.23076915
CGATCGA1051.6568229E-414.0952381
ATTCGTC1203.303318E-513.87499917
CGGGATA1203.303318E-513.87499924
CGTCTGT7800.013.51923134
Produced by FastQC (version 0.11.5)