##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088283_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2414591
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.875986036558572	31.0	30.0	33.0	27.0	34.0
2	30.720668634977933	31.0	31.0	34.0	27.0	34.0
3	30.723325399622546	31.0	30.0	34.0	27.0	34.0
4	34.912281210358195	35.0	35.0	37.0	32.0	37.0
5	34.81631216218399	35.0	35.0	37.0	32.0	37.0
6	34.92907038914665	35.0	35.0	37.0	32.0	37.0
7	34.55008322320426	35.0	35.0	37.0	30.0	37.0
8	34.77896960603266	35.0	35.0	37.0	32.0	37.0
9	36.17451195668335	38.0	35.0	39.0	32.0	39.0
10	36.11282076343364	38.0	35.0	39.0	31.0	39.0
11	36.205879587888795	38.0	35.0	39.0	32.0	39.0
12	35.93698560128817	37.0	35.0	39.0	30.0	39.0
13	36.10985007398769	38.0	35.0	39.0	31.0	39.0
14	36.97745456683968	38.0	36.0	40.0	31.0	41.0
15	37.03326319032913	38.0	36.0	40.0	31.0	41.0
16	36.93967715443319	38.0	36.0	40.0	31.0	41.0
17	37.06331382830467	39.0	36.0	40.0	31.0	41.0
18	37.070350630810765	39.0	36.0	40.0	31.0	41.0
19	37.17981306150814	39.0	36.0	40.0	31.0	41.0
20	37.13964642459116	39.0	36.0	40.0	31.0	41.0
21	37.06684776013826	39.0	36.0	40.0	31.0	41.0
22	37.00067009278176	39.0	36.0	40.0	31.0	41.0
23	36.863908628831965	39.0	35.0	40.0	31.0	41.0
24	36.80016615650435	39.0	35.0	40.0	30.0	41.0
25	36.70927581524159	38.0	35.0	40.0	30.0	41.0
26	36.44156298105973	38.0	35.0	40.0	30.0	41.0
27	36.287249062056475	38.0	35.0	40.0	30.0	41.0
28	36.116338129314656	38.0	35.0	40.0	30.0	41.0
29	35.950426801060715	38.0	34.0	40.0	29.0	41.0
30	35.78915601027255	38.0	34.0	40.0	29.0	41.0
31	35.52345304028715	38.0	34.0	40.0	27.0	41.0
32	35.27544002276162	37.0	34.0	40.0	27.0	41.0
33	35.38206760482417	38.0	34.0	40.0	27.0	41.0
34	35.35909725498024	38.0	34.0	40.0	27.0	41.0
35	35.26495791626822	38.0	34.0	40.0	27.0	41.0
36	35.171947547224356	38.0	34.0	40.0	26.0	41.0
37	35.007045499631204	38.0	33.0	40.0	25.0	41.0
38	34.87596822815955	38.0	33.0	40.0	25.0	41.0
39	34.8190004021385	38.0	33.0	40.0	24.0	41.0
40	34.652418152805176	38.0	33.0	40.0	24.0	41.0
41	34.479103500344365	38.0	33.0	40.0	23.0	41.0
42	34.276973615821476	38.0	33.0	40.0	22.0	41.0
43	33.92720837607694	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	9.0
16	30.0
17	85.0
18	202.0
19	475.0
20	1011.0
21	2028.0
22	3730.0
23	6625.0
24	10635.0
25	16916.0
26	25302.0
27	36508.0
28	50805.0
29	67971.0
30	87009.0
31	105749.0
32	128498.0
33	155133.0
34	185138.0
35	219044.0
36	265990.0
37	330450.0
38	378315.0
39	336927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.779196973731786	25.613240503257074	14.12789992176729	21.479662601243852
2	18.01907652269059	23.39613624005059	36.043826884138966	22.540960353119846
3	20.147760014014796	26.470197230089898	32.28658600980456	21.095456746090747
4	13.28046861766651	16.14778651953892	35.11443552966113	35.45730933313344
5	10.955105854366225	40.963997629412184	34.67870956199207	13.402186954229514
6	31.57350458110711	36.00775452240151	15.22949435328799	17.18924654320338
7	23.73217658808469	35.95296263425152	21.48409399355833	18.830766784105464
8	27.462207885310598	34.07914632333178	20.256515492685924	18.2021302986717
9	24.868186786085097	13.890841140383609	24.197679855511762	37.04329221801953
10	16.36148730778836	28.223951799704384	33.77391864709178	21.640642245415474
11	31.00699041783888	21.966163213562876	25.28241843028488	21.74442793831336
12	19.79589918126921	27.76105766980826	31.990096873549184	20.452946275373346
13	30.84410568912085	21.338769174572423	29.214927082888988	18.602198053417744
14	20.432404494177277	23.187198163167178	28.865799632318684	27.514597710336865
15	22.774208965410704	29.302519557142393	27.34048954874759	20.582781928699312
16	21.232084439973477	28.736502372451483	27.755715150102027	22.275698037473013
17	18.763053452945034	27.492275089238717	28.52980069916603	25.21487075865022
18	19.905814276620763	26.696032578602342	33.103535961162784	20.29461718361412
19	19.20010469682029	25.10768904547395	34.57894111259422	21.11326514511153
20	23.821342827832954	23.938422697674262	33.35256364328369	18.887670831209093
21	23.05959891343917	24.874606092708866	31.854048988006667	20.211746005845296
22	21.314872787979414	24.563994481881195	32.72529384893757	21.395838881201826
23	20.308408339134868	26.876352972408164	33.85111598610282	18.964122702354146
24	19.02318032329285	25.750075271547026	32.25618748682489	22.970556918335237
25	20.109244174272163	26.795469708948637	33.47121727861986	19.62406883815934
26	21.690878496606672	25.585533947571243	31.915508672069098	20.808078883752984
27	20.37802675484171	27.08375869867816	31.564227647663724	20.973986898816406
28	20.024923475652812	26.471522506296097	31.825389890047635	21.67816412800346
29	19.12377707031957	26.327522963516387	32.8280855846808	21.72061438148324
30	18.476586717999034	26.592371130348784	34.811154352848995	20.119887798803195
31	19.881048177517435	26.00167067631744	33.610122790982	20.507158355183133
32	21.001983358672337	26.414245725259473	31.477546300802082	21.106224615266107
33	22.14433003353363	26.19441553455637	30.88589330449753	20.77536112741247
34	20.206900464716384	29.09925531901676	31.002434780880073	19.69140943538678
35	18.88974157528128	29.829358263987565	31.226944853186318	20.05395530754484
36	19.924036824455985	25.99380184884314	33.48231646684677	20.59984485985411
37	19.910038594528018	26.31439444609874	32.56307175832263	21.21249520105061
38	20.2425586776394	25.923437965270306	33.19713359322552	20.63686976386477
39	21.26422238797378	25.347936772728797	33.0955014741627	20.292339365134715
40	19.47116509586924	26.1505571751075	32.19443789859235	22.18383983043091
41	20.45389881764655	25.636722741035644	31.957379117208667	21.951999324109135
42	17.890524730689382	27.393624841639845	32.34825276827421	22.36759765939656
43	18.640879552686147	27.14422442558595	32.010224505930815	22.204671515797088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	248.0
1	319.5
2	391.0
3	1016.5
4	1642.0
5	1642.0
6	3032.0
7	4422.0
8	4934.5
9	5447.0
10	8501.5
11	11556.0
12	11556.0
13	21244.0
14	30932.0
15	50271.5
16	69611.0
17	66933.0
18	64255.0
19	64255.0
20	69016.0
21	73777.0
22	59239.0
23	44701.0
24	48696.0
25	52691.0
26	52691.0
27	58072.5
28	63454.0
29	65870.0
30	68286.0
31	72421.5
32	76557.0
33	76557.0
34	83969.5
35	91382.0
36	98746.5
37	106111.0
38	110579.5
39	115048.0
40	115048.0
41	119955.0
42	124862.0
43	143275.0
44	161688.0
45	204082.0
46	246476.0
47	246476.0
48	247685.0
49	248894.0
50	224455.0
51	200016.0
52	184636.5
53	169257.0
54	169257.0
55	145620.5
56	121984.0
57	99326.5
58	76669.0
59	66413.5
60	56158.0
61	56158.0
62	48924.5
63	41691.0
64	35935.0
65	30179.0
66	25459.5
67	20740.0
68	20740.0
69	17295.0
70	13850.0
71	11528.5
72	9207.0
73	7396.5
74	5586.0
75	5586.0
76	4418.0
77	3250.0
78	2499.0
79	1748.0
80	1361.0
81	974.0
82	974.0
83	705.5
84	437.0
85	334.0
86	231.0
87	172.0
88	113.0
89	113.0
90	82.0
91	51.0
92	30.5
93	10.0
94	9.0
95	8.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2414591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.62018826055735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.14911352752146	30.44479876108326
2	13.78566007596257	11.475235353152229
3	5.358021597065958	6.69005602722052
4	2.564971086857141	4.270183180715224
5	1.3986139391946064	2.910528772655961
6	0.846629879024861	2.1142136971216563
7	0.5252662252877939	1.5303175428373217
8	0.36902942276849987	1.2287259239447819
9	0.26811192229526387	1.0042981812745924
>10	1.4961345509096005	11.625645214627244
>50	0.13961196486300823	4.050569201912105
>100	0.08381215103262887	6.837292526359111
>500	0.0062514546807570185	1.8714139715459284
>1k	0.007259753822814599	5.887186177788313
>5k	0.0010082991420575834	3.104657062137043
>10k+	5.041495710287917E-4	4.954878405624696
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33465	1.3859490075130738	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30847	1.2775248478934942	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26465	1.0960448374072462	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15646	0.647977235067968	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11647	0.4823591241746532	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9600	0.39758286185942054	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8995	0.3725268585859883	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8900	0.36859244484883774	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8841	0.36614896684366005	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7149	0.2960749874409372	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6716	0.2781423437758196	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6578	0.27242709013659044	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6412	0.26555221981693794	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5398	0.22355753003303666	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5392	0.2233090407443745	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4581	0.18972157189354222	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4431	0.18350933967698876	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4077	0.16884847164592265	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4070	0.16855856747581682	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3735	0.1546845821921808	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3420	0.14163889453741857	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3088	0.1278891538981136	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3086	0.12780632413522622	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3031	0.12552850565582327	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2967	0.12287795324342715	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2955	0.12238097466610287	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2656	0.10999792511443968	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2565	0.1062291709030639	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2497	0.10341295896489301	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2430	0.10063816190816581	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.141488144368964E-5	0.0
3	0.0	0.0	0.0	4.141488144368964E-5	0.0
4	0.0	0.0	0.0	4.141488144368964E-5	0.0
5	0.0	0.0	0.0	4.141488144368964E-5	0.0
6	0.0	0.0	0.0	4.141488144368964E-5	0.0
7	0.0	0.0	0.0	8.282976288737928E-5	0.0
8	4.141488144368964E-5	0.0	0.0	8.282976288737928E-5	0.0
9	4.141488144368964E-5	0.0	0.0	2.070744072184482E-4	0.0
10	4.141488144368964E-5	0.0	0.0	4.55563695880586E-4	0.0
11	4.141488144368964E-5	0.0	0.0	6.626381030990342E-4	0.0
12	4.141488144368964E-5	0.0	0.0	9.11127391761172E-4	0.0
13	4.141488144368964E-5	0.0	0.0	0.0012010315618669995	0.0
14	4.141488144368964E-5	0.0	0.0	0.0014081059690854475	0.0
15	4.141488144368964E-5	0.0	0.0	0.0025677226495087573	0.0
16	4.141488144368964E-5	0.0	0.0	0.004141488144368963	0.0
17	4.141488144368964E-5	0.0	0.0	0.006460721505215583	0.0
18	4.141488144368964E-5	0.0	0.0	0.007040529845427238	0.0
19	8.282976288737928E-5	0.0	0.0	0.008448635814512686	0.0
20	8.282976288737928E-5	0.0	0.0	0.010436550123809788	0.0
21	8.282976288737928E-5	0.0	0.0	0.015323506134165166	0.0
22	8.282976288737928E-5	0.0	0.0	0.02633986459818661	0.0
23	8.282976288737928E-5	0.0	0.0	0.04191186002101391	0.0
24	8.282976288737928E-5	0.0	0.0	0.063116279320183	0.0
25	8.282976288737928E-5	0.0	0.0	0.07301443598522482	0.0
26	8.282976288737928E-5	0.0	0.0	0.08581163435132493	0.0
27	8.282976288737928E-5	0.0	0.0	0.17178892822842462	0.0
28	8.282976288737928E-5	0.0	0.0	0.25598538220344563	0.0
29	8.282976288737928E-5	0.0	0.0	0.34908603568885993	0.0
30	8.282976288737928E-5	0.0	0.0	0.5407955218916992	0.0
31	8.282976288737928E-5	0.0	0.0	0.7983960844714488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7520	0.0	30.456118	1
ATTGGAC	755	0.0	21.562912	3
GTATTGG	735	0.0	20.891155	1
TATTGGA	885	0.0	19.231638	2
GTATCAA	12990	0.0	18.215166	2
GGACCCT	1425	0.0	18.045614	6
TTGGACC	1470	0.0	17.870749	4
GTTCGCG	65	0.001580615	17.076923	37
TGGACCC	1555	0.0	16.655947	5
TCGACGA	80	3.3844952E-4	16.1875	37
GACCCTC	1630	0.0	15.549079	7
ACGGACC	120	1.9375511E-6	15.416666	8
CTAGTAC	265	0.0	15.358491	3
AGTATCA	460	0.0	14.880435	1
TTTTTAC	1625	0.0	14.686153	1
CGAATTA	130	4.448553E-6	14.230769	15
CGATCGA	105	1.6568229E-4	14.095238	1
ATTCGTC	120	3.303318E-5	13.874999	17
CGGGATA	120	3.303318E-5	13.874999	24
CGTCTGT	780	0.0	13.519231	34
>>END_MODULE