FastQCFastQC Report
Wed 25 May 2016
SRR2088282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248047
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT381371.696450296635257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT343501.5279929645599046No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT308461.3721243372580734No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG187020.8319221083900825No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136630.6077719905322264No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT116310.517382421275No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT113840.5063951065080046No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA99230.44140536207650466No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA86910.38660223740873745No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86910.38660223740873745No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT81150.36097999730432684No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT74920.33326705358028547No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG72800.3238366457640788No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60610.2696118008208903No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA59120.26298382551610355No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT52700.23442570373306254No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50030.22254872785133054No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45390.2019085899894442No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC44400.1975047674714986No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39300.17481840904571835No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38730.17228287486871938No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG35350.15724760202967286No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34810.1548455170198844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.14123370196441623No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31120.1384312694529963No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC30300.13478365888257676No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30080.13380503165636662No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA28200.1254422171778437No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC27960.12437462384015993No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC26670.11863630965010963No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC25370.11285351240432251No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC25320.11263109712563839No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC24460.10880555433227153No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG24410.10858313905358741No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG23930.10644795237821986No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC23030.10244447736190568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88600.029.1281031
TAGCGAG400.001931758723.12528
ATTGGAC8250.022.6484853
CGCGATA502.703216E-422.214
ACGTTTA1400.021.14285726
ACGTGCG705.105505E-621.1428579
GTATTGG8100.021.0123461
TTGGACC14000.019.5571444
TGGACCC14350.018.564465
CGCGATC609.240562E-418.510
TGCGCGA609.240562E-418.512
TATTGGA9950.018.4070362
GGACCCT14400.018.3715296
TCACGTT1451.546141E-1017.8620724
ACGGTGT1054.802314E-717.61904729
GTATCAA152050.017.5570532
AGTATCA4800.017.343751
GACCCTC14900.017.2583897
TTTTTAC17900.015.9162021
CTGCGTG957.064474E-515.5789489
Produced by FastQC (version 0.11.5)