##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2248047
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.838336565027333	31.0	30.0	33.0	27.0	34.0
2	30.665155577263288	31.0	30.0	34.0	27.0	34.0
3	30.695790168088124	31.0	30.0	34.0	27.0	34.0
4	34.88360519152847	35.0	35.0	37.0	32.0	37.0
5	34.78450939860243	35.0	35.0	37.0	32.0	37.0
6	34.89748746356282	35.0	35.0	37.0	32.0	37.0
7	34.508213573826524	35.0	35.0	37.0	30.0	37.0
8	34.75234058718523	35.0	35.0	37.0	31.0	37.0
9	36.11036868891086	38.0	35.0	39.0	31.0	39.0
10	36.06437320927899	37.0	35.0	39.0	31.0	39.0
11	36.16946398362668	38.0	35.0	39.0	31.0	39.0
12	35.907959219713824	37.0	35.0	39.0	30.0	39.0
13	36.07180810721484	37.0	35.0	39.0	31.0	39.0
14	36.90692810248184	38.0	36.0	40.0	31.0	41.0
15	36.94781915146792	38.0	36.0	40.0	31.0	41.0
16	36.830993747016855	38.0	36.0	40.0	31.0	41.0
17	37.00478326298338	38.0	36.0	40.0	31.0	41.0
18	37.02256892315864	38.0	36.0	40.0	31.0	41.0
19	37.1372542477982	39.0	36.0	40.0	31.0	41.0
20	37.10086977718882	39.0	36.0	40.0	31.0	41.0
21	37.02219704481268	39.0	36.0	40.0	31.0	41.0
22	36.962381124593925	39.0	36.0	40.0	31.0	41.0
23	36.82148816283645	39.0	35.0	40.0	31.0	41.0
24	36.75783113075483	38.0	35.0	40.0	30.0	41.0
25	36.66205199446453	38.0	35.0	40.0	30.0	41.0
26	36.374742165088186	38.0	35.0	40.0	30.0	41.0
27	36.2046198322366	38.0	35.0	40.0	30.0	41.0
28	36.01130180997105	38.0	34.0	40.0	30.0	41.0
29	35.83936590293708	38.0	34.0	40.0	29.0	41.0
30	35.66535352686132	38.0	34.0	40.0	29.0	41.0
31	35.37479198611061	38.0	34.0	40.0	27.0	41.0
32	35.14619934547632	37.0	34.0	40.0	27.0	41.0
33	35.19852387427843	37.0	33.0	40.0	27.0	41.0
34	35.15706744565394	37.0	33.0	40.0	27.0	41.0
35	35.02199598140074	37.0	33.0	40.0	26.0	41.0
36	34.911434236027986	37.0	33.0	40.0	25.0	41.0
37	34.72523261301921	37.0	33.0	40.0	25.0	41.0
38	34.56352291566858	37.0	33.0	40.0	24.0	41.0
39	34.4641740141554	37.0	33.0	40.0	24.0	41.0
40	34.25109706336211	37.0	33.0	40.0	23.0	41.0
41	34.060093939317106	37.0	33.0	40.0	21.0	41.0
42	33.817582105712205	37.0	33.0	40.0	20.0	41.0
43	33.45349808077856	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	0.0
13	1.0
14	2.0
15	14.0
16	29.0
17	91.0
18	229.0
19	495.0
20	1144.0
21	2206.0
22	3938.0
23	6613.0
24	10979.0
25	16966.0
26	25708.0
27	36523.0
28	50157.0
29	67133.0
30	84725.0
31	102448.0
32	122892.0
33	146880.0
34	174295.0
35	204755.0
36	246946.0
37	306491.0
38	341618.0
39	294766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.483249238116464	25.569839064752646	14.19191858533207	20.754993111798818
2	18.252287429933627	23.17625031860989	35.90169600546608	22.66976624599041
3	20.40219799675007	26.598376279499497	32.440069091082165	20.559356632668266
4	12.997948886299977	15.913412842347158	34.291809735294684	36.796828536058186
5	10.529005843739032	41.62386284628391	34.13972216773048	13.707409142246583
6	31.606100762128193	35.410202722629904	14.90791785047199	18.07577866476991
7	23.570815022995514	36.7154245440598	20.92376182526433	18.789998607680356
8	28.481344028839256	33.447743752688446	19.679214891859466	18.39169732661283
9	24.970474371754683	13.521514452322394	24.508918185429394	36.99909299049352
10	16.539734267121638	28.41239529244718	32.91843987247597	22.129430567955207
11	30.420983191187727	21.512050237383825	25.577801531729545	22.489165039698904
12	20.17248749692511	28.108753953987613	31.93220604373485	19.78655250535242
13	31.611483211872347	20.883771558156923	29.753737355135367	17.751007874835356
14	20.6698080600628	22.995293247872485	29.373540677752736	26.961358014311976
15	23.225893408812183	28.58885957455516	28.2801471677416	19.90509984889106
16	21.164459639856283	28.395936561824552	28.167871935061857	22.27173186325731
17	18.348103931990746	27.23795365488355	28.546378256326495	25.86756415679921
18	19.52512558678711	26.089000808257122	34.128245539350374	20.25762806560539
19	18.781324411811674	24.318753122154472	36.1262909538813	20.773631512152548
20	24.42511210842122	22.598771289034435	34.57623439367593	18.39988220886841
21	23.498752472701863	23.889358185126913	32.91252362606298	19.69936571610825
22	21.362720619275308	23.353203914330972	34.0295821217261	21.254493344667615
23	20.172176115534953	25.991449467026268	35.23035772828593	18.606016689152852
24	18.340808710849906	24.90668566982808	33.38649058493884	23.366015034383178
25	19.905010882779585	26.14109046652494	34.97787190392372	18.976026746771755
26	21.7729433592803	24.54459359613033	33.11065115631479	20.571811888274578
27	20.3031787146799	26.446555610269712	32.484552146819	20.765713528231394
28	19.6103996046346	25.79994991207924	32.87320060479163	21.716449878494533
29	18.588712780471226	25.712051393943273	34.0940825525445	21.605153273041
30	17.772760089090664	25.946388131564863	36.55372863645645	19.727123142888026
31	19.508355474774326	25.29493378029908	34.744424827416864	20.452285917509734
32	21.103962684054203	25.78002150310914	32.2471905614073	20.868825251429353
33	22.35055583802296	25.46592664655143	31.65712282705833	20.52639468836728
34	19.998647715105598	29.02216902048756	31.795776511790013	19.183406752616825
35	18.263630609146517	29.992211016940484	32.00395721263835	19.740201161274655
36	19.448125417306667	25.4472882461977	34.91862047368227	20.185965862813365
37	19.586512203703926	25.943674665164917	33.635284315674895	20.83452881545626
38	20.105318082762505	25.49782099751473	34.151332245277786	20.245528674444973
39	21.290524619814445	25.024254386140505	33.7220262743617	19.96319471968335
40	19.32397320874519	25.839495348629278	32.63268072242262	22.203850720202915
41	20.38565030001597	25.527669127914145	32.349901937103624	21.73677863496626
42	17.37717227442309	27.433234269568207	32.92484543250208	22.264748023506627
43	18.330444158863227	27.149654789245954	32.646559435812506	21.873341616078314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	258.0
2	342.0
3	1017.0
4	1692.0
5	1692.0
6	3151.0
7	4610.0
8	5361.5
9	6113.0
10	9442.0
11	12771.0
12	12771.0
13	23751.0
14	34731.0
15	55900.5
16	77070.0
17	73570.5
18	70071.0
19	70071.0
20	73504.0
21	76937.0
22	59186.5
23	41436.0
24	43430.0
25	45424.0
26	45424.0
27	48529.5
28	51635.0
29	53242.0
30	54849.0
31	57957.5
32	61066.0
33	61066.0
34	66725.0
35	72384.0
36	78821.5
37	85259.0
38	90084.0
39	94909.0
40	94909.0
41	101123.5
42	107338.0
43	127906.0
44	148474.0
45	198672.0
46	248870.0
47	248870.0
48	250232.5
49	251595.0
50	224820.5
51	198046.0
52	180650.5
53	163255.0
54	163255.0
55	138629.0
56	114003.0
57	90660.0
58	67317.0
59	58053.0
60	48789.0
61	48789.0
62	42550.0
63	36311.0
64	31066.5
65	25822.0
66	21483.0
67	17144.0
68	17144.0
69	14488.0
70	11832.0
71	9750.0
72	7668.0
73	6107.5
74	4547.0
75	4547.0
76	3591.0
77	2635.0
78	2048.5
79	1462.0
80	1136.5
81	811.0
82	811.0
83	568.0
84	325.0
85	244.0
86	163.0
87	126.5
88	90.0
89	90.0
90	73.0
91	56.0
92	33.0
93	10.0
94	8.5
95	7.0
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2248047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.29585194938491
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0220132186249	29.08758072434497
2	13.333609389245005	10.479110810214005
3	5.099723788436196	6.011939729194352
4	2.40071013251032	3.7735179976205506
5	1.2765850641287364	2.5082248840399433
6	0.8149791717861777	1.9215180525805187
7	0.5401067915363373	1.4856769561948449
8	0.37675146525048486	1.1843815840157508
9	0.26727558085185876	0.9452539489356451
>10	1.5967980501661416	12.050327243159664
>50	0.16527988872233812	4.509308506878448
>100	0.08642741275254237	6.4776569765548215
>500	0.00895188132058952	2.3937169218049767
>1k	0.008837113611351194	6.747118479199599
>5k	0.001147677092383272	3.266877675484841
>10k+	8.033739646682904E-4	7.157789509776989
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38137	1.696450296635257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34350	1.5279929645599046	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30846	1.3721243372580734	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18702	0.8319221083900825	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13663	0.6077719905322264	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11631	0.517382421275	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11384	0.5063951065080046	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9923	0.44140536207650466	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8691	0.38660223740873745	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8691	0.38660223740873745	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8115	0.36097999730432684	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7492	0.33326705358028547	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7280	0.3238366457640788	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6061	0.2696118008208903	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5912	0.26298382551610355	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5270	0.23442570373306254	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5003	0.22254872785133054	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4539	0.2019085899894442	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4440	0.1975047674714986	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3930	0.17481840904571835	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3873	0.17228287486871938	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3535	0.15724760202967286	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3481	0.1548455170198844	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3175	0.14123370196441623	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3112	0.1384312694529963	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3030	0.13478365888257676	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3008	0.13380503165636662	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2820	0.1254422171778437	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2796	0.12437462384015993	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2667	0.11863630965010963	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2537	0.11285351240432251	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2532	0.11263109712563839	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2446	0.10880555433227153	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	2441	0.10858313905358741	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2393	0.10644795237821986	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2303	0.10244447736190568	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.4483055736824004E-5	0.0
3	0.0	0.0	0.0	4.4483055736824004E-5	0.0
4	0.0	0.0	0.0	1.3344916721047203E-4	0.0
5	0.0	0.0	0.0	1.7793222294729602E-4	0.0
6	4.4483055736824004E-5	0.0	0.0	2.2241527868412003E-4	0.0
7	4.4483055736824004E-5	0.0	0.0	2.6689833442094407E-4	0.0
8	4.4483055736824004E-5	0.0	0.0	2.6689833442094407E-4	0.0
9	4.4483055736824004E-5	0.0	0.0	7.562119475260081E-4	0.0
10	4.4483055736824004E-5	0.0	0.0	0.0011120763934206002	0.0
11	4.4483055736824004E-5	0.0	0.0	0.0017348391737361363	0.0
12	4.4483055736824004E-5	0.0	0.0	0.0024465680655253205	0.0
13	4.4483055736824004E-5	0.0	0.0	0.0027134663999462644	0.0
14	8.896611147364801E-5	0.0	0.0	0.0033807122359986244	0.0
15	8.896611147364801E-5	0.0	0.0	0.004804170019576993	0.0
16	8.896611147364801E-5	0.0	0.0	0.006761424471997249	0.0
17	8.896611147364801E-5	0.0	0.0	0.009341441704733042	0.0
18	8.896611147364801E-5	0.0	0.0	0.010409035042416817	0.0
19	8.896611147364801E-5	0.0	0.0	0.01187697588173201	0.0
20	8.896611147364801E-5	0.0	0.0	0.015747001730835698	0.0
21	8.896611147364801E-5	0.0	0.0	0.021485315920885997	0.0
22	8.896611147364801E-5	0.0	0.0	0.03563092764519603	0.0
23	8.896611147364801E-5	0.0	0.0	0.06187593052992219	0.0
24	8.896611147364801E-5	0.0	0.0	0.09550512066696114	0.0
25	8.896611147364801E-5	0.0	0.0	0.10827175766342964	0.0
26	8.896611147364801E-5	0.0	0.0	0.12161667438447683	0.0
27	8.896611147364801E-5	0.0	0.0	0.20315411555007523	0.0
28	8.896611147364801E-5	0.0	0.0	0.2848250058828841	0.0
29	1.3344916721047203E-4	0.0	0.0	0.3838887710087912	0.0
30	1.3344916721047203E-4	0.0	0.0	0.5752103937328712	0.0
31	1.3344916721047203E-4	0.0	0.0	0.828052082540979	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8860	0.0	29.128103	1
TAGCGAG	40	0.0019317587	23.125	28
ATTGGAC	825	0.0	22.648485	3
CGCGATA	50	2.703216E-4	22.2	14
ACGTTTA	140	0.0	21.142857	26
ACGTGCG	70	5.105505E-6	21.142857	9
GTATTGG	810	0.0	21.012346	1
TTGGACC	1400	0.0	19.557144	4
TGGACCC	1435	0.0	18.56446	5
CGCGATC	60	9.240562E-4	18.5	10
TGCGCGA	60	9.240562E-4	18.5	12
TATTGGA	995	0.0	18.407036	2
GGACCCT	1440	0.0	18.371529	6
TCACGTT	145	1.546141E-10	17.86207	24
ACGGTGT	105	4.802314E-7	17.619047	29
GTATCAA	15205	0.0	17.557053	2
AGTATCA	480	0.0	17.34375	1
GACCCTC	1490	0.0	17.258389	7
TTTTTAC	1790	0.0	15.916202	1
CTGCGTG	95	7.064474E-5	15.578948	9
>>END_MODULE