FastQCFastQC Report
Wed 25 May 2016
SRR2088281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504696
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68771.3626024379032131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64451.2770063563016152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55391.0974923518315975No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG29010.5748014646440629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.48425190609792823No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT19580.3879563142961307No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17210.34099735286192084No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT16980.3364401540729469No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16600.3289108691172508No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT13940.27620587442737804No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA13750.27244123194953No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT13070.2589677746603896No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG11720.2322189991598903No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11180.2215194889596906No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10980.21755670740406108No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT9680.19179862729246913No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8780.17396611029213627No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8400.16643682533644016No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7550.14959500372501466No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7210.14285827508044446No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7170.14206571876931856No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.13295132119137065No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6190.12264808914673388No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6150.12185553283560796No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG6100.1208648374467006No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT5840.1157132214243822No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.10699510200199724No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG5080.10065465151299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.029.4806461
ATTGGAC1250.023.683
CGTCTGT2050.022.56097434
GGACCCT2400.021.5833326
TTGGACC2250.021.3777774
AGTATCA1059.786163E-1021.1428591
GTATTGG1251.8189894E-1120.721
GGACTAC450.003822879220.5555551
CGCCGGA555.1376E-420.18181814
TGGACCC2400.020.0416665
ACCGACC759.248888E-619.7333348
GACCCTC2750.018.8363637
CACATTA609.227031E-418.527
ACCGCCG801.6137436E-518.512
GGGTAGG609.227031E-418.51
CGACCGC801.6137436E-518.510
GCCGGAA609.227031E-418.515
CCGACCG902.1473516E-618.59
CTACAGT701.21747864E-418.54
TATACCG609.227031E-418.55
Produced by FastQC (version 0.11.5)