##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504696
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.883012744305482	31.0	30.0	33.0	27.0	34.0
2	30.791846973227447	31.0	31.0	34.0	27.0	34.0
3	30.704267519457257	31.0	31.0	34.0	27.0	34.0
4	34.962668616355195	35.0	35.0	37.0	32.0	37.0
5	34.91540650213198	35.0	35.0	37.0	32.0	37.0
6	35.04360248545659	36.0	35.0	37.0	32.0	37.0
7	34.58646591215306	35.0	35.0	37.0	30.0	37.0
8	34.87094409307781	35.0	35.0	37.0	32.0	37.0
9	36.28599394486978	38.0	35.0	39.0	32.0	39.0
10	36.23612828316452	38.0	35.0	39.0	32.0	39.0
11	36.33477776721036	38.0	35.0	39.0	32.0	39.0
12	36.08400898758857	38.0	35.0	39.0	31.0	39.0
13	36.24143048488595	38.0	35.0	39.0	32.0	39.0
14	37.13048845245455	39.0	36.0	40.0	31.0	41.0
15	37.17836479781889	39.0	36.0	40.0	32.0	41.0
16	37.11066463772251	39.0	36.0	40.0	31.0	41.0
17	37.219419214735204	39.0	36.0	40.0	32.0	41.0
18	37.22744384738536	39.0	36.0	40.0	32.0	41.0
19	37.322063182589126	39.0	36.0	40.0	32.0	41.0
20	37.28717683516414	39.0	36.0	40.0	31.0	41.0
21	37.22332651734906	39.0	36.0	40.0	31.0	41.0
22	37.14908776768589	39.0	36.0	40.0	31.0	41.0
23	37.00756296859892	39.0	36.0	40.0	31.0	41.0
24	36.92870361564189	39.0	35.0	40.0	31.0	41.0
25	36.847535942428706	39.0	35.0	40.0	31.0	41.0
26	36.5886236467101	38.0	35.0	40.0	30.0	41.0
27	36.4301856959437	38.0	35.0	40.0	30.0	41.0
28	36.272718626658424	38.0	35.0	40.0	30.0	41.0
29	36.10426870829173	38.0	35.0	40.0	30.0	41.0
30	35.917447334633124	38.0	34.0	40.0	29.0	41.0
31	35.645652036077166	38.0	34.0	40.0	29.0	41.0
32	35.3468404742657	38.0	34.0	40.0	27.0	41.0
33	35.465177057079906	38.0	34.0	40.0	28.0	41.0
34	35.43680155975082	38.0	34.0	40.0	27.0	41.0
35	35.33982833230301	38.0	34.0	40.0	27.0	41.0
36	35.237943237116994	38.0	34.0	40.0	26.0	41.0
37	35.07371566249782	38.0	34.0	40.0	25.0	41.0
38	34.92014995165407	38.0	33.0	40.0	25.0	41.0
39	34.85298080428615	38.0	33.0	40.0	24.0	41.0
40	34.673952240556694	38.0	33.0	40.0	24.0	41.0
41	34.48823648295211	38.0	33.0	40.0	23.0	41.0
42	34.26337636914103	38.0	33.0	40.0	22.0	41.0
43	33.908103095724954	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	7.0
17	15.0
18	40.0
19	90.0
20	197.0
21	389.0
22	665.0
23	1288.0
24	2021.0
25	3375.0
26	4988.0
27	7105.0
28	10058.0
29	13410.0
30	17513.0
31	21537.0
32	26230.0
33	31900.0
34	38477.0
35	46027.0
36	55691.0
37	70224.0
38	79461.0
39	73986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.01120674623932	25.62275112146718	13.866367080381062	21.49967505191244
2	17.76475343573161	23.28589883811245	36.11401715091857	22.83533057523737
3	19.87493461410433	26.6451487628196	32.335703076703595	21.14421354637247
4	13.088671200088767	16.04153787626611	35.318686892703724	35.5511040309414
5	10.981263968804985	41.07759918842234	34.49462646821056	13.446510374562113
6	31.29527477927307	36.07062469288443	15.452866676177344	17.181233851665162
7	23.72913595510961	35.874070727725204	21.76022793919508	18.636565377970104
8	27.716684895461825	33.66184792429502	20.329267519457257	18.292199660785897
9	24.93144387908761	13.878849842281294	24.37625818314391	36.81344809548718
10	16.35994737426094	28.364599679807252	33.72069523039612	21.55475771553569
11	30.765847163440963	22.187613929969725	25.31464485551698	21.731894051072327
12	19.82896634805903	28.19578518553743	31.773780652115335	20.201467814288204
13	30.630914451471774	21.34908935279852	29.30793982912486	18.71205636660485
14	20.587442737806523	23.331272686924407	29.04639624645331	27.034888328815764
15	22.768161427869448	29.48765197267266	27.427203702823082	20.316982896634805
16	21.376036267376797	28.658836210312742	27.83437950766402	22.13074801464644
17	18.940708862364673	27.76780477752944	28.48586079540951	24.80562556469637
18	19.878104839348836	26.81257628354495	33.11934312932934	20.189975747776877
19	19.176890643080192	25.578962385277475	34.70009669186996	20.544050279772378
20	23.63363291961894	24.28610490275334	33.281222755876804	18.799039421750916
21	22.96372469763977	25.24846640353797	31.806473599949275	19.981335298872985
22	21.149761440550353	24.994055827666557	32.72009288759967	21.136089844183427
23	20.26546673641162	27.09888725093918	33.6834450837732	18.952200928876
24	18.911582417930795	26.283941224023966	32.24257771014631	22.561898647898936
25	20.006895239906797	27.165660120151536	33.492637151869644	19.334807488072027
26	21.457075150189418	26.11096580912074	31.906533834228924	20.525425206460916
27	20.191758999476914	27.511610949957994	31.638847940146146	20.657782110418946
28	19.85868720972625	27.18884239225197	31.61744891974575	21.33502147827603
29	18.866406708196617	27.029736712793444	32.72980170241095	21.374054876598983
30	18.26406391174093	27.11315326453945	34.85840981501735	19.764373008702268
31	19.76675067763565	26.52150997828396	33.443498660579834	20.26824068350056
32	20.83036124716661	27.04043622299364	31.355112780763072	20.774089749076673
33	21.616973385959074	26.976041022714664	30.87442737806521	20.53255821326105
34	19.91515684689397	29.548084391396007	30.986375957011745	19.550382804698273
35	18.629828649325532	30.2423637199423	31.130026788403313	19.997780842328847
36	19.68630621205637	26.782657282799942	33.26041815270975	20.270618352433942
37	19.795877122069523	26.91501418675797	32.373745779637645	20.915362911534867
38	19.99520503431769	26.684974717453674	32.89445527604736	20.425364972181274
39	21.020376622759045	26.037059933108246	32.76863696165613	20.17392648247658
40	19.29220758634901	26.842495284289946	32.01531218793095	21.84998494143009
41	20.175907873254395	26.24688921647883	31.835600044383156	21.74160286588362
42	17.73523071314217	27.915616529554427	32.377906700271055	21.97124605703235
43	18.410686829299223	27.68953984180576	31.92357379491813	21.97619953397689
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35.0
1	61.0
2	87.0
3	247.0
4	407.0
5	407.0
6	712.0
7	1017.0
8	1146.0
9	1275.0
10	1952.5
11	2630.0
12	2630.0
13	4702.5
14	6775.0
15	10875.5
16	14976.0
17	14361.5
18	13747.0
19	13747.0
20	14892.0
21	16037.0
22	13079.5
23	10122.0
24	11004.0
25	11886.0
26	11886.0
27	12803.0
28	13720.0
29	14227.0
30	14734.0
31	15738.0
32	16742.0
33	16742.0
34	18057.5
35	19373.0
36	20685.5
37	21998.0
38	22891.0
39	23784.0
40	23784.0
41	24668.5
42	25553.0
43	29372.0
44	33191.0
45	41655.5
46	50120.0
47	50120.0
48	50999.5
49	51879.0
50	46524.0
51	41169.0
52	38051.5
53	34934.0
54	34934.0
55	30102.0
56	25270.0
57	20540.5
58	15811.0
59	13709.0
60	11607.0
61	11607.0
62	9997.5
63	8388.0
64	7379.0
65	6370.0
66	5204.5
67	4039.0
68	4039.0
69	3389.5
70	2740.0
71	2229.5
72	1719.0
73	1430.5
74	1142.0
75	1142.0
76	907.0
77	672.0
78	494.0
79	316.0
80	251.0
81	186.0
82	186.0
83	152.5
84	119.0
85	94.0
86	69.0
87	51.0
88	33.0
89	33.0
90	24.5
91	16.0
92	11.5
93	7.0
94	4.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	504696.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.57367745293625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.8417111093549	47.432339214029064
2	9.565062967248265	10.822615742522574
3	2.7416615948339818	4.653176362537215
4	1.1503913100504544	2.6032746767782076
5	0.6369717878749329	1.8017918236928294
6	0.37720935684387624	1.2804072291788993
7	0.27163449233134435	1.0757153507971895
8	0.1791371098836844	0.8107556059528608
9	0.16085454170900843	0.819011966353679
>10	0.9392720800470109	9.786786829862082
>50	0.07034450996422797	2.770754659084801
>100	0.05549792988043859	6.496824720124553
>500	0.004948859989338473	1.9058312427366946
>1k	0.004241879990861548	3.9688485669834668
>5k	0.001060469997715387	3.7718660093658753
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6877	1.3626024379032131	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6445	1.2770063563016152	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5539	1.0974923518315975	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2901	0.5748014646440629	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2444	0.48425190609792823	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1958	0.3879563142961307	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1721	0.34099735286192084	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1698	0.3364401540729469	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1660	0.3289108691172508	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1394	0.27620587442737804	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1375	0.27244123194953	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1307	0.2589677746603896	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1172	0.2322189991598903	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1118	0.2215194889596906	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1098	0.21755670740406108	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	968	0.19179862729246913	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	878	0.17396611029213627	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	840	0.16643682533644016	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	755	0.14959500372501466	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	721	0.14285827508044446	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	717	0.14206571876931856	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	671	0.13295132119137065	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	619	0.12264808914673388	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	615	0.12185553283560796	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	610	0.1208648374467006	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	584	0.1157132214243822	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.10699510200199724	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	508	0.10065465151299	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	3.962781555629527E-4	0.0
12	0.0	0.0	0.0	7.925563111259055E-4	0.0
13	0.0	0.0	0.0	0.0011888344666888583	0.0
14	0.0	0.0	0.0	0.0011888344666888583	0.0
15	0.0	0.0	0.0	0.0017832517000332874	0.0
16	0.0	0.0	0.0	0.003170225244503622	0.0
17	0.0	0.0	0.0	0.00435905971119248	0.0
18	0.0	0.0	0.0	0.00435905971119248	0.0
19	0.0	0.0	0.0	0.005349755100099862	0.0
20	0.0	0.0	0.0	0.007331145877914626	0.0
21	0.0	0.0	0.0	0.009708814811292342	0.0
22	0.0	0.0	0.0	0.014860430833610728	0.0
23	0.0	0.0	0.0	0.026946914578280787	0.0
24	0.0	0.0	0.0	0.04438315342305071	0.0
25	0.0	0.0	0.0	0.04973290852315057	0.0
26	0.0	0.0	0.0	0.05944172333444291	0.0
27	0.0	0.0	0.0	0.13433829473584097	0.0
28	0.0	0.0	0.0	0.20982928337058349	0.0
29	0.0	0.0	0.0	0.29324583511658503	0.0
30	0.0	0.0	0.0	0.4577012696752104	0.0
31	0.0	0.0	0.0	0.6788244804793381	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1550	0.0	29.480646	1
ATTGGAC	125	0.0	23.68	3
CGTCTGT	205	0.0	22.560974	34
GGACCCT	240	0.0	21.583332	6
TTGGACC	225	0.0	21.377777	4
AGTATCA	105	9.786163E-10	21.142859	1
GTATTGG	125	1.8189894E-11	20.72	1
GGACTAC	45	0.0038228792	20.555555	1
CGCCGGA	55	5.1376E-4	20.181818	14
TGGACCC	240	0.0	20.041666	5
ACCGACC	75	9.248888E-6	19.733334	8
GACCCTC	275	0.0	18.836363	7
CACATTA	60	9.227031E-4	18.5	27
ACCGCCG	80	1.6137436E-5	18.5	12
GGGTAGG	60	9.227031E-4	18.5	1
CGACCGC	80	1.6137436E-5	18.5	10
GCCGGAA	60	9.227031E-4	18.5	15
CCGACCG	90	2.1473516E-6	18.5	9
CTACAGT	70	1.21747864E-4	18.5	4
TATACCG	60	9.227031E-4	18.5	5
>>END_MODULE