FastQCFastQC Report
Wed 25 May 2016
SRR2088280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1418947
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171741.2103341421490725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165331.1651597980756152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135340.9538058856320919No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG62270.43884655311297743No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59490.4192545598954718No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT41140.2899333097007851No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT36430.25673968090421984No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA34520.243278994916653No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT30800.21706237089898356No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA29660.2090282441838913No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27540.19408758748564958No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT26650.1878153306642179No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG25600.18041547711084346No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22670.15976636195714147No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA21540.15180271003779564No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT21300.15011131493988147No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20820.14672852474405315No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17630.12424706490094416No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17070.12030047633914447No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC15450.10888355942822389No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14440.10176560505783515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.027.1567691
ACCGTCA509.093052E-625.9000028
ACGCCTA450.003826243620.5555556
CCGGTCG555.1439117E-420.18181820
ATTGGAC3000.019.7333343
GTATCGG951.676799E-719.47368436
CAATGCG801.6171058E-518.519
TATCGGC609.2382723E-418.537
CTAGGCA2000.017.5754
AGCCGTC1800.017.47222112
ATAACGC852.7238442E-517.4117643
TATCGTG752.0676965E-417.2666667
GTATCAA68900.017.1574752
GCCGTCA1851.8189894E-1217.013
GTATTGG3050.016.9836061
CGGGATA1801.0913936E-1116.44444524
GTCTAAC803.383264E-416.18751
TTAGTAC803.383264E-416.18753
CTAGCAC1606.2937033E-1016.18753
TCTAGGC1059.346108E-615.8571423
Produced by FastQC (version 0.11.5)