FastQCFastQC Report
Wed 25 May 2016
SRR2088279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2384498
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323911.3583991263569941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT301201.263158954211746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT255081.0697429815416075No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG155160.6507029991218277No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108140.45351264710643496No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA97720.4098137217980472No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT97550.40910078347727696No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92390.3874610085644861No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT89590.3757184950459174No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA70950.29754690505087444No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT67660.28374945166655624No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG64080.26873580938210057No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62910.2638291162332701No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA54790.22977582702942084No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51810.21727843764180133No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA50660.21245561958953205No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT46900.19668710143602555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38930.16326287545638538No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38510.16150149842860007No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38330.16074662255954922No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG33810.1417908507367169No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33090.13877134726051354No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31660.13277427785638737No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC30550.12811920999724052No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30340.12723852148334786No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27860.11683800950975845No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26370.11058931481594868No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA24840.1041728699290165No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.10090174116312951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70350.030.320541
AGTATCA4300.021.5116271
GTATTGG8800.021.0227281
ATTGGAC8900.020.5786513
TTGGACC14950.018.6856174
GTATCAA121650.018.3555282
TGGACCC15850.017.8580445
CGTCTGT6950.017.83453434
ACGTTTA1351.1532393E-917.81481426
GGACCCT15700.017.7929946
TGCGACG1054.802605E-717.61904722
CAATGCG1054.802605E-717.61904719
ATCACGT1301.3951649E-817.07692323
CGATCTG2600.017.07692333
TATTGGA10850.017.0506942
GACCCTC16950.016.4808277
CGAATTA1352.2226232E-816.44444315
CGCTTAT803.3844722E-416.18751
TATACGG957.0647606E-515.5789482
CTAGTAC2350.014.9574473
Produced by FastQC (version 0.11.5)