##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2384498
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.73668671561058	31.0	30.0	31.0	27.0	34.0
2	30.636123410462076	31.0	30.0	34.0	27.0	34.0
3	30.605953957604495	31.0	30.0	34.0	27.0	34.0
4	34.84610765033143	35.0	35.0	37.0	32.0	37.0
5	34.77503944226416	35.0	35.0	37.0	32.0	37.0
6	34.89898544683199	35.0	35.0	37.0	32.0	37.0
7	34.469932874760225	35.0	35.0	37.0	30.0	37.0
8	34.73310189398356	35.0	35.0	37.0	31.0	37.0
9	36.11760336976588	38.0	35.0	39.0	31.0	39.0
10	36.053058547333656	37.0	35.0	39.0	31.0	39.0
11	36.15866987516869	38.0	35.0	39.0	31.0	39.0
12	35.889502109039306	37.0	35.0	39.0	30.0	39.0
13	36.06375807402648	37.0	35.0	39.0	31.0	39.0
14	36.92962711648322	38.0	36.0	40.0	31.0	41.0
15	36.97174667372336	38.0	36.0	40.0	31.0	41.0
16	36.892303537264446	38.0	36.0	40.0	31.0	41.0
17	37.00157727119083	38.0	36.0	40.0	31.0	41.0
18	37.01087901939947	38.0	36.0	40.0	31.0	41.0
19	37.105106819129226	39.0	36.0	40.0	31.0	41.0
20	37.06425880835295	39.0	36.0	40.0	31.0	41.0
21	37.00517719033524	39.0	36.0	40.0	31.0	41.0
22	36.92053379788953	39.0	36.0	40.0	31.0	41.0
23	36.796506853853515	39.0	35.0	40.0	31.0	41.0
24	36.711351823318786	38.0	35.0	40.0	30.0	41.0
25	36.63043290453588	38.0	35.0	40.0	30.0	41.0
26	36.36567571035916	38.0	35.0	40.0	30.0	41.0
27	36.200985280759305	38.0	35.0	40.0	30.0	41.0
28	36.04700024910904	38.0	34.0	40.0	29.0	41.0
29	35.872960681870985	38.0	34.0	40.0	29.0	41.0
30	35.702383897994466	38.0	34.0	40.0	29.0	41.0
31	35.43040002549803	38.0	34.0	40.0	27.0	41.0
32	35.1738797851791	37.0	34.0	40.0	27.0	41.0
33	35.2944510752368	38.0	34.0	40.0	27.0	41.0
34	35.275928098912225	38.0	34.0	40.0	27.0	41.0
35	35.17765458389984	38.0	33.0	40.0	26.0	41.0
36	35.08039847380874	38.0	33.0	40.0	26.0	41.0
37	34.92604061735426	38.0	33.0	40.0	25.0	41.0
38	34.78595914108546	38.0	33.0	40.0	25.0	41.0
39	34.72570704609524	38.0	33.0	40.0	24.0	41.0
40	34.557793715910016	38.0	33.0	40.0	24.0	41.0
41	34.390054007174676	38.0	33.0	40.0	23.0	41.0
42	34.17426309437039	38.0	33.0	40.0	22.0	41.0
43	33.81690318045979	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	7.0
15	9.0
16	24.0
17	93.0
18	240.0
19	508.0
20	1154.0
21	2153.0
22	3982.0
23	6921.0
24	10858.0
25	17256.0
26	25878.0
27	37161.0
28	51461.0
29	68306.0
30	87262.0
31	106918.0
32	129124.0
33	155366.0
34	186529.0
35	220422.0
36	265471.0
37	326081.0
38	368948.0
39	312364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.67560383778892	25.91518214735345	13.886109361383403	21.52310465347423
2	18.085483820913247	23.51853513821358	35.767570364915386	22.62841067595779
3	20.101337891665246	26.591173488088476	32.49715453734916	20.810334082897114
4	13.167593346691842	16.195861770485863	35.35079501010275	35.28574987271954
5	11.029323572508764	40.92593074097777	34.68151367709262	13.36323200942085
6	31.5533500132942	36.18975566345621	15.187934735109865	17.068959588139727
7	23.885237060379165	35.8799629943074	21.396201632377128	18.83859831293631
8	27.775238226243008	33.75851017698484	20.175147976639106	18.291103620133043
9	24.91652331014746	13.7146686640123	24.269636627919166	37.09917139792107
10	16.3015024546047	28.374441916076254	33.78136614079777	21.542689488521276
11	31.20501673727552	21.762358366415068	25.298784062725154	21.73384083358426
12	20.00312015359208	27.885282352931306	31.889018149732145	20.22257934374447
13	31.153391615342095	20.986849223610168	29.201827806104262	18.65793135494347
14	20.6771823671062	22.932122400605913	28.897864456166456	27.49283077612143
15	22.976156826300546	29.213863882460796	27.11484765346836	20.6951316377703
16	21.55225124952925	28.56886438990513	27.579977001448526	22.2989073591171
17	19.08942678920259	27.4059781136323	28.400946446589597	25.10364865057551
18	20.166508841693304	26.473874165547635	33.03663915843083	20.32297783432823
19	19.430882307303257	24.985720264810453	34.55280734141945	21.030590086466837
20	23.97326397422015	23.810546286891412	33.162703428562324	19.053486310326115
21	23.32302228812941	24.714300452338396	31.808288369291986	20.15438889024021
22	21.570326332838192	24.59352031329026	32.60011121837804	21.23604213549351
23	20.70641283825778	26.69106872809287	33.49799412706574	19.10452430658361
24	19.216371747847973	25.657937226200232	32.07949849402264	23.04619253192915
25	20.458226427533173	26.638940355580083	33.225945251369474	19.67688796551727
26	21.834281261716303	25.48364477554605	31.830515269880706	20.851558692856944
27	20.5839971348267	27.019355856033428	31.4513159583275	20.94533105081237
28	20.279572471857808	26.62199758607472	31.45630652657289	21.64212341549458
29	19.28703651670079	26.491404060728925	32.46393999911093	21.757619423459364
30	18.672903059679648	26.704362930897823	34.55414934296443	20.068584666458097
31	20.253655067020397	26.035249348080814	33.22569362607979	20.485401958819004
32	21.3301080562869	26.50364982482686	31.066413140208127	21.09982897867811
33	22.330821833358634	26.37775330488849	30.485787784263184	20.805637077489685
34	20.403162426640744	29.304197361457213	30.605309796862905	19.68733041503914
35	18.98470873114593	30.296859129259072	30.77364711566124	19.944785023933758
36	20.15359207682288	26.514301962090137	33.091325721388735	20.24078023969825
37	20.18982611853732	26.90935366689341	32.05265846312306	20.848161751446217
38	20.554892476319964	26.4644382171845	32.51233592982674	20.4683333766688
39	21.620106202647264	25.81298034219362	32.44284541232578	20.124068042833336
40	19.7274227111954	26.798261101498095	31.463561722425432	22.010754464881078
41	20.767809408940582	26.14420309851382	31.439699257453768	21.64828823509183
42	18.24849507107995	27.839067174726082	31.9191712469459	21.993266507248066
43	18.86166396449064	27.69325031935443	31.472704107950605	21.972381608204326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	347.0
2	410.0
3	1013.0
4	1616.0
5	1616.0
6	2874.0
7	4132.0
8	4684.0
9	5236.0
10	7870.5
11	10505.0
12	10505.0
13	19514.5
14	28524.0
15	47686.0
16	66848.0
17	64321.5
18	61795.0
19	61795.0
20	67046.5
21	72298.0
22	59258.0
23	46218.0
24	50203.5
25	54189.0
26	54189.0
27	58685.0
28	63181.0
29	65577.5
30	67974.0
31	72980.5
32	77987.0
33	77987.0
34	84664.5
35	91342.0
36	98349.0
37	105356.0
38	108377.5
39	111399.0
40	111399.0
41	116296.5
42	121194.0
43	137836.0
44	154478.0
45	195184.0
46	235890.0
47	235890.0
48	241298.5
49	246707.0
50	223419.5
51	200132.0
52	184811.5
53	169491.0
54	169491.0
55	145651.5
56	121812.0
57	98735.0
58	75658.0
59	65720.0
60	55782.0
61	55782.0
62	48930.5
63	42079.0
64	36477.0
65	30875.0
66	26457.5
67	22040.0
68	22040.0
69	18486.0
70	14932.0
71	12383.5
72	9835.0
73	8005.5
74	6176.0
75	6176.0
76	4855.0
77	3534.0
78	2834.5
79	2135.0
80	1710.0
81	1285.0
82	1285.0
83	950.5
84	616.0
85	445.5
86	275.0
87	214.0
88	153.0
89	153.0
90	122.5
91	92.0
92	55.5
93	19.0
94	13.5
95	8.0
96	8.0
97	7.0
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2384498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.68899407493574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39354145565487	29.456203793527564
2	14.04958290020146	11.433267907632315
3	5.538329943466038	6.7604722276418645
4	2.624836779485899	4.272078726727007
5	1.4594726558993676	2.969223712421005
6	0.8878682548696875	2.167587969702975
7	0.5795585868157472	1.650715913351685
8	0.4157047677454311	1.3531687065373108
9	0.27480551151377614	1.006340384676935
>10	1.5320290128553233	11.586308756333413
>50	0.1352531638053744	3.9036052268793213
>100	0.09187849750427299	7.179784973766863
>500	0.008046720756524297	2.2768808409260703
>1k	0.007419703554717205	5.719947162705192
>5k	0.0011495315366463275	3.4021609607136862
>10k+	5.225143348392397E-4	4.8622527364568535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32391	1.3583991263569941	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30120	1.263158954211746	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25508	1.0697429815416075	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15516	0.6507029991218277	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10814	0.45351264710643496	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9772	0.4098137217980472	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9755	0.40910078347727696	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9239	0.3874610085644861	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8959	0.3757184950459174	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7095	0.29754690505087444	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6766	0.28374945166655624	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6408	0.26873580938210057	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6291	0.2638291162332701	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5479	0.22977582702942084	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5181	0.21727843764180133	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5066	0.21245561958953205	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4690	0.19668710143602555	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3893	0.16326287545638538	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3851	0.16150149842860007	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3833	0.16074662255954922	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3381	0.1417908507367169	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3309	0.13877134726051354	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3166	0.13277427785638737	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3055	0.12811920999724052	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3034	0.12723852148334786	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2786	0.11683800950975845	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2637	0.11058931481594868	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2484	0.1041728699290165	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2406	0.10090174116312951	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.258126448418074E-4	0.0
10	0.0	0.0	0.0	2.516252896836148E-4	0.0
11	0.0	0.0	0.0	3.774379345254221E-4	0.0
12	0.0	0.0	0.0	4.193754828060246E-4	0.0
13	0.0	0.0	0.0	4.61313031086627E-4	0.0
14	0.0	0.0	0.0	5.032505793672295E-4	0.0
15	0.0	0.0	0.0	7.968134173314467E-4	0.0
16	0.0	0.0	0.0	0.0017194394795047008	0.0
17	0.0	0.0	0.0	0.0026840030899585574	0.0
18	0.0	0.0	0.0	0.0031872536693257868	0.0
19	0.0	0.0	0.0	0.0038163168935348235	0.0
20	0.0	0.0	0.0	0.004571192762585668	0.0
21	0.0	0.0	0.0	0.006500319983493381	0.0
22	0.0	0.0	0.0	0.009477885911416156	0.0
23	0.0	0.0	0.0	0.015307205122419898	0.0
24	0.0	0.0	0.0	0.0238624649716628	0.0
25	0.0	0.0	0.0	0.028853033217054493	0.0
26	0.0	0.0	0.0	0.0357727286833539	0.0
27	0.0	0.0	0.0	0.08890760235487721	0.0
28	0.0	0.0	0.0	0.14766210749600125	0.0
29	0.0	0.0	0.0	0.21673324951415351	0.0
30	0.0	0.0	0.0	0.34732677485994956	0.0
31	0.0	0.0	0.0	0.5234644776384799	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7035	0.0	30.32054	1
AGTATCA	430	0.0	21.511627	1
GTATTGG	880	0.0	21.022728	1
ATTGGAC	890	0.0	20.578651	3
TTGGACC	1495	0.0	18.685617	4
GTATCAA	12165	0.0	18.355528	2
TGGACCC	1585	0.0	17.858044	5
CGTCTGT	695	0.0	17.834534	34
ACGTTTA	135	1.1532393E-9	17.814814	26
GGACCCT	1570	0.0	17.792994	6
TGCGACG	105	4.802605E-7	17.619047	22
CAATGCG	105	4.802605E-7	17.619047	19
ATCACGT	130	1.3951649E-8	17.076923	23
CGATCTG	260	0.0	17.076923	33
TATTGGA	1085	0.0	17.050694	2
GACCCTC	1695	0.0	16.480827	7
CGAATTA	135	2.2226232E-8	16.444443	15
CGCTTAT	80	3.3844722E-4	16.1875	1
TATACGG	95	7.0647606E-5	15.578948	2
CTAGTAC	235	0.0	14.957447	3
>>END_MODULE