FastQCFastQC Report
Wed 25 May 2016
SRR2088278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2963174
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT349491.1794447440481053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325291.0977755609356723No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT288910.9750018054964036No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG168750.5694906880257453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128260.43284667049589387No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA109580.36980616055621435No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA105270.35526094653908274No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT96070.3242131579178273No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT93600.31587750162494677No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA72930.24612122001610434No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67900.2291461790633962No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT67470.22769503242131578No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT65940.22253165018321572No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57140.1928337654150583No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55460.18716416923204646No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA54460.1837894095993013No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43740.1476119863362732No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43660.14734200556565358No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38520.12999574105334347No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37630.1269922049802003No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC37520.12662098142059833No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34660.11696916887094716No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33750.11389813760514908No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33640.1135269140455471No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29990.10120904138602729No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC29950.10107405100071747No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29910.10093906061540765No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29750.10039909907416844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83500.027.0077841
GTATTGG12600.019.2341271
CGACGAG1650.019.06060624
ATTGGAC13500.017.5407413
TATTGGA14750.017.183052
CGTCTGT7800.017.07692134
GTATCAA144150.016.2604942
TTGGACC20550.015.3941614
GTAACAC2201.8189894E-1215.1363643
CGAATTA1853.0559022E-1015.015
GGACCCT21800.014.7660556
TGCGACG2151.2732926E-1114.62790722
TGGACCC21950.014.3280195
TATACTG3500.014.2714295
GTATTAG3900.014.2307681
ACGAGTC2009.858923E-1013.87500126
CAATGCG2151.9826984E-1013.76744219
TTTTTAC18700.013.7513381
AGTATCA6300.013.5079371
TCTATAC2750.013.4545463
Produced by FastQC (version 0.11.5)