##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2963174
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.87182257943678	31.0	30.0	33.0	27.0	34.0
2	30.721109188998014	31.0	31.0	34.0	27.0	34.0
3	30.688749968783473	31.0	31.0	34.0	27.0	34.0
4	34.89959347645464	35.0	35.0	37.0	32.0	37.0
5	34.81473885772486	35.0	35.0	37.0	32.0	37.0
6	34.93824189872076	35.0	35.0	37.0	32.0	37.0
7	34.5171515408815	35.0	35.0	37.0	30.0	37.0
8	34.77102694610576	35.0	35.0	37.0	32.0	37.0
9	36.21169833428614	38.0	35.0	39.0	32.0	39.0
10	36.12589507062359	38.0	35.0	39.0	31.0	39.0
11	36.22134069750882	38.0	35.0	39.0	32.0	39.0
12	35.948623334303015	37.0	35.0	39.0	30.0	39.0
13	36.12943958066587	38.0	35.0	39.0	31.0	39.0
14	37.02883563368199	39.0	36.0	40.0	31.0	41.0
15	37.066466565918844	39.0	36.0	40.0	31.0	41.0
16	36.99223299070524	38.0	36.0	40.0	31.0	41.0
17	37.0684968213139	39.0	36.0	40.0	31.0	41.0
18	37.05808028823147	39.0	36.0	40.0	31.0	41.0
19	37.14323256076086	39.0	36.0	40.0	31.0	41.0
20	37.09746339566964	39.0	36.0	40.0	31.0	41.0
21	37.04027404398122	39.0	36.0	40.0	31.0	41.0
22	36.952103723912266	39.0	36.0	40.0	31.0	41.0
23	36.821862975309585	39.0	35.0	40.0	31.0	41.0
24	36.743250311996526	38.0	35.0	40.0	30.0	41.0
25	36.66284160160693	38.0	35.0	40.0	30.0	41.0
26	36.40067846167657	38.0	35.0	40.0	30.0	41.0
27	36.24434238421368	38.0	35.0	40.0	30.0	41.0
28	36.081548704193544	38.0	34.0	40.0	30.0	41.0
29	35.91111119360524	38.0	34.0	40.0	29.0	41.0
30	35.73703602960879	38.0	34.0	40.0	29.0	41.0
31	35.49625334185573	38.0	34.0	40.0	27.0	41.0
32	35.25457195561246	38.0	34.0	40.0	27.0	41.0
33	35.40698521247824	38.0	34.0	40.0	27.0	41.0
34	35.40695787692522	38.0	34.0	40.0	27.0	41.0
35	35.30850500173125	38.0	34.0	40.0	27.0	41.0
36	35.226031275922374	38.0	34.0	40.0	26.0	41.0
37	35.07115545695258	38.0	33.0	40.0	26.0	41.0
38	34.94767772665392	38.0	33.0	40.0	25.0	41.0
39	34.916300561492505	38.0	33.0	40.0	25.0	41.0
40	34.75958414861901	38.0	33.0	40.0	24.0	41.0
41	34.6033540386086	38.0	33.0	40.0	24.0	41.0
42	34.41192653553251	38.0	33.0	40.0	23.0	41.0
43	34.06088842572188	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	5.0
15	18.0
16	40.0
17	106.0
18	255.0
19	617.0
20	1244.0
21	2615.0
22	4596.0
23	8179.0
24	13310.0
25	20898.0
26	30892.0
27	44240.0
28	61014.0
29	81493.0
30	104916.0
31	128832.0
32	156206.0
33	189368.0
34	227285.0
35	272524.0
36	329746.0
37	407122.0
38	475442.0
39	402210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.48981733776012	25.189340889195165	13.352270234552543	21.96857153849217
2	18.315191750467573	23.28884500201473	35.4802654180956	22.9156978294221
3	20.120384425619285	26.85731583767946	31.738568170482058	21.283731566219195
4	13.49627122808178	16.09814340973564	35.46177173530815	34.94381362687442
5	11.45771392432574	40.482064164979846	34.667454560548926	13.392767350145485
6	31.869306358654605	36.25834999902132	15.292048323858134	16.580295318465943
7	24.545774227230666	35.00601044690592	21.748334724859223	18.699880601004192
8	27.65956369757564	33.68026987277831	20.283520306266187	18.37664612337986
9	25.178642901159364	13.529073891712063	23.42542152435193	37.866861682776644
10	16.337143886926654	27.659934921135243	33.87614092186284	22.126780270075265
11	31.840823387354234	21.657823671508996	24.959249777434604	21.542103163702166
12	20.101722004850203	27.13742088719731	31.867720221627216	20.893136886325273
13	30.920155211945026	20.741070217273773	28.770433325886362	19.56834124489483
14	20.989722507014438	22.343946052442416	28.291318700825535	28.375012739717615
15	23.186826018316843	28.87336349468509	26.560910699135455	21.37889978786261
16	21.98834762994006	28.158825637643957	27.134012379968237	22.718814352447747
17	19.736134293834922	26.87452711180646	28.06558102899121	25.323757565367405
18	20.77039687848233	26.10329329293521	32.11897107628509	21.007338752297365
19	20.12733643046274	24.712588595877257	33.284343072664655	21.87573190099535
20	24.206003427405882	23.876525644460973	32.0303498883292	19.88712103980394
21	23.681025818936046	24.604832520803704	30.84972397840964	20.86441768185061
22	22.20841570559137	24.2757259614184	31.75102103352689	21.764837299463345
23	21.26159314302839	26.201127574688492	32.58863637437423	19.948642907908884
24	20.048164569478537	25.393176371012977	31.1706636194837	23.387995440024785
25	21.100752098931753	26.24823921916162	32.07314184047242	20.57786684143422
26	22.420384358124092	25.330844560596173	30.899096711836698	21.349674369443036
27	21.23759860203957	26.642579882247887	30.54869541916877	21.571126096543775
28	21.01132096866401	26.11932340119075	30.744768953831265	22.124586676313978
29	20.082384632154575	26.073460417781742	31.619405407849825	22.224749542213857
30	19.396802212762395	26.4204532032206	33.24404844264967	20.93869614136733
31	20.781871061233666	25.673922624861046	32.41392506818702	21.130281245718272
32	21.721809114145845	26.032254602665926	30.35231815613933	21.8936181270489
33	22.66144343869108	25.970294015808726	29.92112511786348	21.44713742763672
34	21.040985105835837	28.445376478060354	30.0508508781462	20.46278753795761
35	19.87493815752973	29.413156297942677	30.169034960484943	20.542870584042653
36	20.802997056534647	26.00805757609914	32.13483244655899	21.054112920807217
37	20.80481942673633	26.331562034494095	31.310176182701387	21.55344235606819
38	21.146581334744432	25.854944731561496	31.78382369715717	21.214650236536905
39	21.943395831631893	25.36516586606119	31.80417349774262	20.887264804564296
40	20.377136138478537	26.1393019782166	31.01039628452463	22.473165598780227
41	21.26206560937697	25.607406112499635	30.870849973710623	22.25967830441277
42	19.041035052278403	27.252567685866573	31.25449264876109	22.45190461309393
43	19.614609199459768	26.990382609998605	30.883336584351778	22.511671606189847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	356.5
2	473.0
3	1222.5
4	1972.0
5	1972.0
6	3484.0
7	4996.0
8	5557.5
9	6119.0
10	9308.0
11	12497.0
12	12497.0
13	23158.5
14	33820.0
15	53887.5
16	73955.0
17	71587.5
18	69220.0
19	69220.0
20	74041.0
21	78862.0
22	64459.5
23	50057.0
24	55425.5
25	60794.0
26	60794.0
27	66647.0
28	72500.0
29	76922.5
30	81345.0
31	86488.5
32	91632.0
33	91632.0
34	98978.0
35	106324.0
36	115528.0
37	124732.0
38	131534.5
39	138337.0
40	138337.0
41	146243.5
42	154150.0
43	173769.0
44	193388.0
45	237274.0
46	281160.0
47	281160.0
48	289178.5
49	297197.0
50	272429.5
51	247662.0
52	232374.5
53	217087.0
54	217087.0
55	188860.0
56	160633.0
57	134769.5
58	108906.0
59	95876.0
60	82846.0
61	82846.0
62	73129.0
63	63412.0
64	55908.0
65	48404.0
66	41579.5
67	34755.0
68	34755.0
69	29754.5
70	24754.0
71	20874.5
72	16995.0
73	13648.5
74	10302.0
75	10302.0
76	8179.0
77	6056.0
78	4769.5
79	3483.0
80	2721.0
81	1959.0
82	1959.0
83	1459.0
84	959.0
85	766.0
86	573.0
87	455.0
88	337.0
89	337.0
90	269.5
91	202.0
92	128.0
93	54.0
94	36.0
95	18.0
96	18.0
97	12.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2963174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.743052050923666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.986893595091	30.46695698375111
2	13.977796366701703	11.669517625848817
3	5.312033565686573	6.652214808861247
4	2.493127460293434	4.162829973784638
5	1.394617842863979	2.9107802602908976
6	0.8482313125620207	2.1244658298899837
7	0.5340329272340045	1.5604514994905327
8	0.3730162996511619	1.2456671049745112
9	0.27381968254759687	1.0287062335036559
>10	1.538842159352135	12.167807487438171
>50	0.15438882329977002	4.508284270250137
>100	0.09986499825955925	7.757524337171315
>500	0.006135720762492277	1.7843626708995732
>1k	0.005890291931986233	4.766701313454383
>5k	7.362864914982791E-4	2.154751707061306
>10k+	5.726672711653283E-4	5.038977893329809
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34949	1.1794447440481053	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32529	1.0977755609356723	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28891	0.9750018054964036	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16875	0.5694906880257453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12826	0.43284667049589387	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10958	0.36980616055621435	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10527	0.35526094653908274	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9607	0.3242131579178273	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9360	0.31587750162494677	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7293	0.24612122001610434	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6790	0.2291461790633962	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6747	0.22769503242131578	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6594	0.22253165018321572	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5714	0.1928337654150583	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5546	0.18716416923204646	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5446	0.1837894095993013	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4374	0.1476119863362732	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4366	0.14734200556565358	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3852	0.12999574105334347	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3763	0.1269922049802003	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3752	0.12662098142059833	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3466	0.11696916887094716	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3375	0.11389813760514908	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3364	0.1135269140455471	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2999	0.10120904138602729	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2995	0.10107405100071747	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2991	0.10093906061540765	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2975	0.10039909907416844	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.374759632745158E-5	0.0
3	0.0	0.0	0.0	3.374759632745158E-5	0.0
4	0.0	0.0	0.0	3.374759632745158E-5	0.0
5	0.0	0.0	0.0	3.374759632745158E-5	3.374759632745158E-5
6	0.0	0.0	0.0	3.374759632745158E-5	3.374759632745158E-5
7	0.0	0.0	0.0	3.374759632745158E-5	3.374759632745158E-5
8	0.0	0.0	0.0	3.374759632745158E-5	3.374759632745158E-5
9	0.0	0.0	0.0	1.0124278898235473E-4	3.374759632745158E-5
10	0.0	0.0	0.0	1.6873798163725788E-4	3.374759632745158E-5
11	0.0	0.0	0.0	3.7122355960196736E-4	3.374759632745158E-5
12	0.0	0.0	0.0	4.049711559294189E-4	3.374759632745158E-5
13	0.0	0.0	0.0	4.049711559294189E-4	3.374759632745158E-5
14	0.0	0.0	0.0	5.062139449117736E-4	3.374759632745158E-5
15	0.0	0.0	0.0	9.786802934960957E-4	3.374759632745158E-5
16	0.0	0.0	0.0	0.001754875009027482	3.374759632745158E-5
17	0.0	0.0	0.0	0.003037283669470642	3.374759632745158E-5
18	0.0	0.0	0.0	0.0036447404033647705	3.374759632745158E-5
19	0.0	0.0	0.0	0.004049711559294189	3.374759632745158E-5
20	0.0	0.0	0.0	0.00492714906380793	3.374759632745158E-5
21	0.0	0.0	0.0	0.006918257247127573	3.374759632745158E-5
22	0.0	0.0	0.0	0.011372939962351182	3.374759632745158E-5
23	0.0	0.0	0.0	0.02136222847527685	3.374759632745158E-5
24	0.0	0.0	0.0	0.03334262517152216	3.374759632745158E-5
25	0.0	0.0	0.0	0.03894472616187912	3.374759632745158E-5
26	0.0	0.0	0.0	0.0470441492804675	3.374759632745158E-5
27	0.0	0.0	0.0	0.10772232747722543	3.374759632745158E-5
28	0.0	0.0	0.0	0.16418205613305192	3.374759632745158E-5
29	0.0	0.0	0.0	0.23093480166875113	3.374759632745158E-5
30	0.0	0.0	0.0	0.3548222277868259	3.374759632745158E-5
31	0.0	0.0	0.0	0.5305797094601937	3.374759632745158E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8350	0.0	27.007784	1
GTATTGG	1260	0.0	19.234127	1
CGACGAG	165	0.0	19.060606	24
ATTGGAC	1350	0.0	17.540741	3
TATTGGA	1475	0.0	17.18305	2
CGTCTGT	780	0.0	17.076921	34
GTATCAA	14415	0.0	16.260494	2
TTGGACC	2055	0.0	15.394161	4
GTAACAC	220	1.8189894E-12	15.136364	3
CGAATTA	185	3.0559022E-10	15.0	15
GGACCCT	2180	0.0	14.766055	6
TGCGACG	215	1.2732926E-11	14.627907	22
TGGACCC	2195	0.0	14.328019	5
TATACTG	350	0.0	14.271429	5
GTATTAG	390	0.0	14.230768	1
ACGAGTC	200	9.858923E-10	13.875001	26
CAATGCG	215	1.9826984E-10	13.767442	19
TTTTTAC	1870	0.0	13.751338	1
AGTATCA	630	0.0	13.507937	1
TCTATAC	275	0.0	13.454546	3
>>END_MODULE