FastQCFastQC Report
Wed 25 May 2016
SRR2088277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350161
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300161.2771890947045756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278071.1831955342633973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251371.0695862964282021No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG156130.6643374645396635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102220.434948924775792No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA84790.36078379311034436No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT84420.3592094328856619No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT82700.3518907853547055No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA82190.3497207212612242No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA64130.27287492218618215No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT63640.27078995864538646No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT59550.2533868956211936No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG59370.25262099064702376No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48140.20483703031409337No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47900.20381582368186688No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44500.1893487297253252No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT41100.1748816357687835No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36320.1545426036769396No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35310.15024502576631982No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34470.14667080255352719No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31210.13279941246578425No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG28970.12326815056500383No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG28170.11986412845758226No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26830.11416239142765112No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25400.10807770191063507No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.10139730852482022No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC23560.10024845106356543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.029.1056041
GTATTGG8350.022.5988031
ATTGGAC8850.020.9039543
CGATCGT759.272531E-619.73333426
ACGCATA1153.0559022E-919.3043487
CGCCGGA1750.019.02857214
TATTGGA10800.019.013892
TATACTG3200.018.55
CCTATCG609.2407304E-418.513
TTGGACC14000.017.8392874
TCGACGG752.068425E-417.26666619
GTATCAA121850.017.2322542
ACGTTTA2800.017.17857226
CGTCTGT5800.016.90517234
GGACCCT14900.016.7617456
TGCGACG1005.8830483E-616.65000222
CGCCGTT904.449139E-516.44444525
CTGTGCG1659.749783E-1015.696979
ACAACGC3200.015.6093753
GACCCTC15500.015.2774187
Produced by FastQC (version 0.11.5)