##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2350161
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.928649569114626	31.0	30.0	33.0	27.0	34.0
2	30.80036091144394	31.0	31.0	34.0	27.0	34.0
3	30.7305563320981	31.0	31.0	34.0	27.0	34.0
4	34.966796317358686	35.0	35.0	37.0	32.0	37.0
5	34.918814072737995	35.0	35.0	37.0	32.0	37.0
6	35.036639617456	36.0	35.0	37.0	32.0	37.0
7	34.61488000183817	35.0	35.0	37.0	30.0	37.0
8	34.86780948198868	35.0	35.0	37.0	32.0	37.0
9	36.303712809462844	38.0	35.0	39.0	32.0	39.0
10	36.239124893996625	38.0	35.0	39.0	32.0	39.0
11	36.32804858901156	38.0	35.0	39.0	32.0	39.0
12	36.07276310005995	38.0	35.0	39.0	31.0	39.0
13	36.23201346631146	38.0	35.0	39.0	32.0	39.0
14	37.14096183197662	39.0	36.0	40.0	31.0	41.0
15	37.18468947446579	39.0	36.0	40.0	32.0	41.0
16	37.11856294100702	39.0	36.0	40.0	31.0	41.0
17	37.214455094778614	39.0	36.0	40.0	32.0	41.0
18	37.22344469166155	39.0	36.0	40.0	32.0	41.0
19	37.31911600949893	39.0	36.0	40.0	32.0	41.0
20	37.27645382592937	39.0	36.0	40.0	31.0	41.0
21	37.214202346137135	39.0	36.0	40.0	31.0	41.0
22	37.13006725922182	39.0	36.0	40.0	31.0	41.0
23	37.003908668384845	39.0	36.0	40.0	31.0	41.0
24	36.93185956196192	39.0	35.0	40.0	31.0	41.0
25	36.85322707678325	39.0	35.0	40.0	31.0	41.0
26	36.60021675110769	38.0	35.0	40.0	30.0	41.0
27	36.45788139621073	38.0	35.0	40.0	30.0	41.0
28	36.304736143608885	38.0	35.0	40.0	30.0	41.0
29	36.146357632519646	38.0	35.0	40.0	30.0	41.0
30	35.97821808803737	38.0	34.0	40.0	29.0	41.0
31	35.723673824899656	38.0	34.0	40.0	29.0	41.0
32	35.459142160898764	38.0	34.0	40.0	27.0	41.0
33	35.58604878559384	38.0	34.0	40.0	28.0	41.0
34	35.58097296312891	38.0	34.0	40.0	28.0	41.0
35	35.494723552982116	38.0	34.0	40.0	27.0	41.0
36	35.40529308417594	38.0	34.0	40.0	27.0	41.0
37	35.24648694280945	38.0	34.0	40.0	26.0	41.0
38	35.11788979563528	38.0	34.0	40.0	26.0	41.0
39	35.07856993627245	38.0	34.0	40.0	25.0	41.0
40	34.922885708681235	38.0	33.0	40.0	25.0	41.0
41	34.75768723929978	38.0	33.0	40.0	24.0	41.0
42	34.55592404094868	38.0	33.0	40.0	23.0	41.0
43	34.21507760532151	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	6.0
15	6.0
16	22.0
17	61.0
18	174.0
19	410.0
20	840.0
21	1712.0
22	3157.0
23	5396.0
24	9370.0
25	14682.0
26	22086.0
27	32441.0
28	45114.0
29	60899.0
30	79888.0
31	97760.0
32	121028.0
33	146943.0
34	177648.0
35	213172.0
36	259600.0
37	324199.0
38	375051.0
39	358493.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.52124982075696	25.232441522091463	13.479161640415274	21.7671470167363
2	17.768782649358915	23.17449740677341	36.353764699524845	22.702955244342835
3	19.430413490820417	27.333191215410345	32.18715653948814	21.04923875428109
4	12.726362151358991	16.082685399000322	35.97447153620539	35.21648091343529
5	10.862106893953223	40.86409399185843	35.05125819039632	13.222540923792028
6	31.03038472683361	36.64587234661795	15.711349137356972	16.612393789191465
7	24.063202478468497	35.53331027108356	21.982579065859742	18.420908184588207
8	27.52219954292493	33.89257161530635	20.428047269952994	18.157181571815716
9	25.085345216774513	13.685870882888448	23.93623245386167	37.29255144647537
10	16.077494265286507	27.925448511825362	34.11396070311778	21.883096519770348
11	30.96498495209477	22.03389469912912	25.475956753601135	21.525163595174966
12	19.834896417734786	27.770310204279617	32.18673103672472	20.208062341260874
13	30.675132469647824	21.141360102563187	29.12383449474313	19.059672933045864
14	20.58607899629004	22.91000488902675	28.86329915269635	27.640616961986858
15	22.779758493141532	29.156470556698032	27.3352336286748	20.728537321485636
16	21.314795028936313	28.77287981546796	27.63189415533659	22.28043100025913
17	18.985763103038472	27.411483723881048	28.376906943822146	25.225846229258337
18	20.093006394030024	26.734593927820267	32.746394821461166	20.426004856688543
19	19.379991413354233	25.217208523160757	34.12549182800667	21.277308235478333
20	23.930786018489798	24.099200012254478	32.82604893877483	19.143965030480892
21	23.12322432378037	25.051134794594926	31.620727260813197	20.20491362081151
22	21.562182335593178	24.621249352703924	32.59308617579817	21.22348213590473
23	20.520891972932915	26.660726648089216	33.54702082112672	19.271360557851143
24	19.207237291402592	25.768319702352304	32.114735969152754	22.909707037092353
25	20.417026748380216	26.77582514559641	33.05994780783104	19.747200298192336
26	21.894712745211926	25.623563662234204	31.66612840567093	20.81559518688294
27	20.49063872645321	27.22707082621148	31.27955914509687	21.00273130223844
28	20.1881913622088	26.52558697042458	31.51294741083696	21.77327425652966
29	19.286253154571114	26.513375041114205	32.50262428829344	21.69774751602124
30	18.630298094470973	26.88483895358658	34.32526537543598	20.15959757650646
31	20.120536422823797	26.219012229374922	33.24929653755636	20.411154810244916
32	21.23441755692482	26.536139438957584	31.11501722647938	21.114425777638214
33	22.14367441209347	26.373554833051866	30.67721743318862	20.805553321666046
34	20.277121439765192	29.13251475111705	30.778997694200527	19.811366114917234
35	19.07316137064652	30.015518085782205	30.97311205487624	19.938208488695032
36	20.05360483813662	26.43142320887803	32.98310200875599	20.531869944229353
37	20.220827424163705	26.741870025074878	32.018189392131006	21.01911315863041
38	20.493532145244515	26.341471924689415	32.57355559895684	20.591440331109233
39	21.435339961815384	25.80784890907474	32.49262497335289	20.26418615575699
40	19.644271179719176	26.62379300822369	31.703657749405256	22.02827806265188
41	20.772576857500404	26.020387539406876	31.530563225242865	21.67647237784986
42	18.292789302520127	27.79945714357442	31.916494231671788	21.99125932223367
43	18.976572243348436	27.42416370623119	31.55043420429494	22.048829846125436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	254.5
2	341.0
3	1033.0
4	1725.0
5	1725.0
6	2968.0
7	4211.0
8	4594.0
9	4977.0
10	7754.0
11	10531.0
12	10531.0
13	18900.5
14	27270.0
15	44926.5
16	62583.0
17	60769.5
18	58956.0
19	58956.0
20	63594.5
21	68233.0
22	56567.5
23	44902.0
24	49198.0
25	53494.0
26	53494.0
27	58865.5
28	64237.0
29	67037.0
30	69837.0
31	75104.0
32	80371.0
33	80371.0
34	86388.5
35	92406.0
36	99927.0
37	107448.0
38	110521.0
39	113594.0
40	113594.0
41	119338.5
42	125083.0
43	140865.0
44	156647.0
45	194380.0
46	232113.0
47	232113.0
48	233936.5
49	235760.0
50	213457.5
51	191155.0
52	177532.0
53	163909.0
54	163909.0
55	141592.0
56	119275.0
57	98043.0
58	76811.0
59	66631.0
60	56451.0
61	56451.0
62	48973.5
63	41496.0
64	35999.5
65	30503.0
66	25362.0
67	20221.0
68	20221.0
69	17170.0
70	14119.0
71	11612.0
72	9105.0
73	7302.5
74	5500.0
75	5500.0
76	4249.5
77	2999.0
78	2351.5
79	1704.0
80	1335.0
81	966.0
82	966.0
83	696.5
84	427.0
85	360.0
86	293.0
87	241.0
88	189.0
89	189.0
90	146.5
91	104.0
92	68.0
93	32.0
94	22.5
95	13.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2350161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.94081588709335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88570526692997	32.905389877093064
2	13.435373039336673	11.807225061918212
3	4.927381222505483	6.495394533109031
4	2.2758966572957435	4.000190239851338
5	1.2579325853160017	2.7637292064872887
6	0.6816233023820103	1.7970650420592285
7	0.4380630554796837	1.3474193647439237
8	0.31235596969922386	1.0980140924630484
9	0.22034974971021326	0.8714113004505223
>10	1.3294501210454253	11.247916938996894
>50	0.13778239652862578	4.201555347804805
>100	0.08444192628039111	7.028933400130229
>500	0.005987965157743878	1.855097726818145
>1k	0.006380618610710689	5.3827510120231095
>5k	7.853069059336231E-4	2.505166966049828
>10k+	4.908168162085144E-4	4.692739890001397
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30016	1.2771890947045756	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27807	1.1831955342633973	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25137	1.0695862964282021	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15613	0.6643374645396635	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10222	0.434948924775792	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8479	0.36078379311034436	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8442	0.3592094328856619	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8270	0.3518907853547055	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8219	0.3497207212612242	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6413	0.27287492218618215	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6364	0.27078995864538646	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5955	0.2533868956211936	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5937	0.25262099064702376	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4814	0.20483703031409337	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4790	0.20381582368186688	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4450	0.1893487297253252	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4110	0.1748816357687835	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3632	0.1545426036769396	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3531	0.15024502576631982	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3447	0.14667080255352719	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3121	0.13279941246578425	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2897	0.12326815056500383	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2817	0.11986412845758226	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2683	0.11416239142765112	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2540	0.10807770191063507	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2383	0.10139730852482022	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2356	0.10024845106356543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.255027634276971E-5	0.0
3	0.0	0.0	0.0	4.255027634276971E-5	0.0
4	0.0	0.0	0.0	8.510055268553942E-5	0.0
5	0.0	0.0	0.0	8.510055268553942E-5	0.0
6	0.0	0.0	0.0	8.510055268553942E-5	0.0
7	0.0	0.0	0.0	1.7020110537107884E-4	0.0
8	0.0	0.0	0.0	1.7020110537107884E-4	0.0
9	0.0	0.0	0.0	2.5530165805661824E-4	0.0
10	0.0	0.0	0.0	4.6805303977046677E-4	0.0
11	0.0	0.0	0.0	8.510055268553941E-4	0.0
12	0.0	0.0	0.0	0.0010637569085692427	0.0
13	0.0	0.0	0.0	0.0012339580139403216	0.0
14	0.0	0.0	0.0	0.001319058566625861	0.0
15	0.0	0.0	0.0	0.0019573127117674064	0.0
16	0.0	0.0	0.0	0.0031912707257077282	0.0
17	0.0	0.0	0.0	0.0049358320557612865	0.0
18	0.0	0.0	0.0	0.005616636477245601	0.0
19	0.0	0.0	0.0	0.006978245320214232	0.0
20	0.0	0.0	0.0	0.008297303886840093	0.0
21	0.0	0.0	0.0	0.011318373507176743	0.0
22	0.0	0.0	0.0	0.018637021038133132	0.0
23	0.0	0.0	0.0	0.03361471831078807	0.0
24	0.0	0.0	0.0	0.053528247639204295	0.0
25	0.0	0.0	0.0	0.06135749848627392	0.0
26	0.0	0.0	0.0	0.07139936370316757	0.0
27	0.0	0.0	0.0	0.13492692628292274	0.0
28	0.0	0.0	0.0	0.197518382783137	0.0
29	0.0	0.0	0.0	0.27240686914641166	0.0
30	0.0	0.0	0.0	0.4108654683657843	0.0
31	0.0	0.0	0.0	0.5997886953276818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6960	0.0	29.105604	1
GTATTGG	835	0.0	22.598803	1
ATTGGAC	885	0.0	20.903954	3
CGATCGT	75	9.272531E-6	19.733334	26
ACGCATA	115	3.0559022E-9	19.304348	7
CGCCGGA	175	0.0	19.028572	14
TATTGGA	1080	0.0	19.01389	2
TATACTG	320	0.0	18.5	5
CCTATCG	60	9.2407304E-4	18.5	13
TTGGACC	1400	0.0	17.839287	4
TCGACGG	75	2.068425E-4	17.266666	19
GTATCAA	12185	0.0	17.232254	2
ACGTTTA	280	0.0	17.178572	26
CGTCTGT	580	0.0	16.905172	34
GGACCCT	1490	0.0	16.761745	6
TGCGACG	100	5.8830483E-6	16.650002	22
CGCCGTT	90	4.449139E-5	16.444445	25
CTGTGCG	165	9.749783E-10	15.69697	9
ACAACGC	320	0.0	15.609375	3
GACCCTC	1550	0.0	15.277418	7
>>END_MODULE