FastQCFastQC Report
Wed 25 May 2016
SRR2088276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993171
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT292110.9759215226928232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT265420.8867518761874948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240670.8040636502224564No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG160460.5360869793272753No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102980.3440498387830164No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95000.3173891501688343No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93500.31237774253458955No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT88550.2958400973415819No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT84200.28130701520227214No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA67180.22444424324570833No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT62100.20747227605773275No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG60830.20322928426073886No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58480.19537807896708873No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA52830.17650177687810018No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50620.16911830296364624No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45620.1524136108494971No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39240.13109842371184272No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36910.1233140371866492No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.12087515213798344No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33680.11252280608090885No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC32320.10797912982586026No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG31470.10513933216645491No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30520.10196544066476657No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30060.10042860899026483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.029.5833341
GTATTGG10900.020.8761461
ATTGGAC10900.020.706423
TTGGACC17650.017.8186974
GTATCAA116700.017.770782
GGACCCT18700.016.9171126
TGGACCC18900.016.5423285
TATTGGA14500.016.458622
GCGTAAT803.3848334E-416.187511
GACCCTC19650.016.005097
TGCGTAA957.065729E-515.57894610
CGTCTGT5600.014.53571534
GTTATAC1803.3378456E-914.3888873
ACCCTCG21550.014.3364268
TAAACGT2304.0017767E-1113.6739134
TATACTG4650.013.5268815
CTAGTAC3700.013.53
CCCTCGC22550.013.3725069
TTATACT7350.013.3401364
TTTAGCG1254.8494563E-513.32000126