##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2993171
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.990200693511998	31.0	31.0	33.0	27.0	34.0
2	30.859744398165024	31.0	31.0	34.0	27.0	34.0
3	30.827875186549647	31.0	31.0	34.0	27.0	34.0
4	35.0422779052717	35.0	35.0	37.0	32.0	37.0
5	34.97409035434327	35.0	35.0	37.0	32.0	37.0
6	35.08868888546628	36.0	35.0	37.0	32.0	37.0
7	34.68914572538622	35.0	35.0	37.0	31.0	37.0
8	34.93256148746597	36.0	35.0	37.0	32.0	37.0
9	36.41751172919957	38.0	35.0	39.0	32.0	39.0
10	36.327185783906096	38.0	35.0	39.0	32.0	39.0
11	36.415541911905464	38.0	35.0	39.0	32.0	39.0
12	36.16158983232164	38.0	35.0	39.0	31.0	39.0
13	36.33709300270516	38.0	35.0	39.0	32.0	39.0
14	37.28767050061624	39.0	36.0	40.0	32.0	41.0
15	37.32856759603778	39.0	36.0	41.0	32.0	41.0
16	37.258343409046795	39.0	36.0	40.0	32.0	41.0
17	37.313842744033	39.0	36.0	40.0	32.0	41.0
18	37.31093145029135	39.0	36.0	40.0	32.0	41.0
19	37.38433353791013	39.0	36.0	40.0	32.0	41.0
20	37.34111181753398	39.0	36.0	40.0	32.0	41.0
21	37.285134394259465	39.0	36.0	40.0	31.0	41.0
22	37.19456823549339	39.0	36.0	40.0	31.0	41.0
23	37.09460501922543	39.0	36.0	40.0	31.0	41.0
24	37.02028818266648	39.0	36.0	40.0	31.0	41.0
25	36.939995409550605	39.0	36.0	40.0	31.0	41.0
26	36.721524096017234	38.0	35.0	40.0	30.0	41.0
27	36.57874875842376	38.0	35.0	40.0	30.0	41.0
28	36.444587028272025	38.0	35.0	40.0	30.0	41.0
29	36.28229559888159	38.0	35.0	40.0	30.0	41.0
30	36.139088612043885	38.0	35.0	40.0	30.0	41.0
31	35.93073867146247	38.0	34.0	40.0	29.0	41.0
32	35.70538168383965	38.0	34.0	40.0	29.0	41.0
33	35.877391903102094	38.0	34.0	40.0	29.0	41.0
34	35.88604927683718	38.0	34.0	40.0	29.0	41.0
35	35.80104110323132	38.0	34.0	40.0	29.0	41.0
36	35.736737727313276	38.0	34.0	40.0	28.0	41.0
37	35.607594754860315	38.0	34.0	40.0	27.0	41.0
38	35.5029298359499	38.0	34.0	40.0	27.0	41.0
39	35.50049729868424	38.0	34.0	40.0	27.0	41.0
40	35.37835526269632	38.0	34.0	40.0	26.0	41.0
41	35.25040400297878	38.0	34.0	40.0	26.0	41.0
42	35.10089199714951	38.0	34.0	40.0	25.0	41.0
43	34.77446126532697	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	3.0
15	14.0
16	26.0
17	85.0
18	191.0
19	473.0
20	880.0
21	1912.0
22	3466.0
23	6250.0
24	10203.0
25	16443.0
26	25055.0
27	36639.0
28	51818.0
29	71398.0
30	93243.0
31	117665.0
32	146363.0
33	180088.0
34	220573.0
35	268762.0
36	331192.0
37	415363.0
38	502897.0
39	492167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49360059949799	24.765474475063403	12.937115854723972	22.803809070714635
2	18.01457384158807	22.786068687689408	36.02213171248819	23.177225758234325
3	19.492838865537585	26.635865441700457	31.6486762700828	22.22261942267916
4	13.195804716803682	15.96307060304941	36.09406211673172	34.747062563415184
5	11.597700231627261	39.91800000735006	35.221342181920114	13.262957579102563
6	31.78826067738863	36.66576349964637	15.495238995700547	16.050736827264465
7	24.981132050257067	34.37879092106665	22.04685265225408	18.593224376422196
8	27.18595095301939	33.98459359655696	20.34153745308905	18.4879179973346
9	25.337977683199526	13.740812001719915	22.985890214758864	37.935320100321704
10	16.006101889935458	27.546104115000446	34.45349430420113	21.994299690862967
11	32.09726407211616	21.986147801111265	24.642694988024406	21.27389313874817
12	20.036743640774283	27.050843403200155	31.83810747865725	21.074305477368316
13	30.613653546690117	20.97898850416498	28.0619784168696	20.345379532275302
14	20.926435542773866	22.340086817625856	27.991484616147893	28.741993023452384
15	23.098914161603197	29.08340352088137	26.061457898663328	21.756224418852113
16	22.22238555698956	28.305566237278125	26.619628480965506	22.85241972476681
17	19.903239741398	27.215351211140295	27.812944866831863	25.06846418062984
18	20.981260342292504	26.653037865193802	31.214454503267604	21.151247289246086
19	20.423791357059116	25.200865570326584	32.31629599511688	22.05904707749741
20	24.094981542985682	24.594418427814517	31.131164908386456	20.179435120813345
21	23.57566607454101	25.205609702887006	29.995914032308878	21.222810190263104
22	22.23377815701141	24.87629340254867	30.946611469909335	21.943316970530585
23	21.453368350822586	26.518063952911476	31.677207884213765	20.35135981205217
24	20.40748757755571	25.772533543857	30.39448798615248	23.425490892434812
25	21.389923930173047	26.50643748720003	31.34117629764554	20.76246228498138
26	22.479570996779003	25.80590951870107	30.096576506988743	21.617942977531186
27	21.321701967578864	26.940191522636027	29.868256775172554	21.869849734612558
28	21.197084964407313	26.425419730446407	30.010948255211613	22.366547049934667
29	20.423123169374552	26.387199394889233	30.87484811258695	22.31482932314926
30	19.934677971956834	26.75470262140052	32.204374557952086	21.10624484869057
31	21.075775490274363	26.098943227767474	31.453064325426112	21.372216956532053
32	21.81933474565937	26.359503015363973	29.777951209603458	22.043211029373197
33	22.614010358913674	26.210764436779588	29.521300319961675	21.653924884345063
34	21.277501352244826	28.36450039105684	29.57064598046687	20.78735227623146
35	20.245051151437725	29.255795943499386	29.681431498567907	20.817721406494986
36	21.208911886424133	26.262114660338483	31.271050000150343	21.257923453087045
37	21.15729438779141	26.451846553370988	30.62324203996364	21.767617018873963
38	21.231797314620515	26.235119877882017	31.201591890339706	21.331490917157755
39	21.960121890797417	25.676013832821447	31.185455157757442	21.178409118623694
40	20.5608366511636	26.31483466865074	30.60673780415486	22.517590876030805
41	21.42015942289966	25.839151856008225	30.51716724503879	22.223521476053325
42	19.306514729696367	27.359579522853856	30.883400914949394	22.450504832500382
43	19.909821390090976	27.018603347419845	30.601325483909875	22.47024977857931
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	297.5
2	402.0
3	1057.0
4	1712.0
5	1712.0
6	3022.5
7	4333.0
8	4672.0
9	5011.0
10	7756.5
11	10502.0
12	10502.0
13	19152.0
14	27802.0
15	44415.5
16	61029.0
17	60130.0
18	59231.0
19	59231.0
20	64781.0
21	70331.0
22	59508.0
23	48685.0
24	54551.5
25	60418.0
26	60418.0
27	67700.0
28	74982.0
29	81075.5
30	87169.0
31	95002.0
32	102835.0
33	102835.0
34	112274.5
35	121714.0
36	130627.5
37	139541.0
38	145813.0
39	152085.0
40	152085.0
41	159424.0
42	166763.0
43	183935.5
44	201108.0
45	238854.0
46	276600.0
47	276600.0
48	280443.0
49	284286.0
50	261988.0
51	239690.0
52	226390.5
53	213091.0
54	213091.0
55	187177.5
56	161264.0
57	136712.5
58	112161.0
59	100550.5
60	88940.0
61	88940.0
62	78564.0
63	68188.0
64	59144.5
65	50101.0
66	42959.0
67	35817.0
68	35817.0
69	30359.5
70	24902.0
71	21108.0
72	17314.0
73	13985.0
74	10656.0
75	10656.0
76	8544.0
77	6432.0
78	5073.0
79	3714.0
80	2881.5
81	2049.0
82	2049.0
83	1549.0
84	1049.0
85	789.0
86	529.0
87	422.0
88	315.0
89	315.0
90	245.0
91	175.0
92	106.0
93	37.0
94	23.5
95	10.0
96	10.0
97	7.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2993171.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.3311728435558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4396237881959	33.2910427950842
2	14.206994673379105	12.880394622528494
3	5.208205305798956	7.082821647656905
4	2.3530754944527454	4.266706878118916
5	1.313264170762192	2.976590255703495
6	0.7567266576493462	2.0581984147937273
7	0.5207249730689931	1.6523551630702527
8	0.3275903657789558	1.1880044394407607
9	0.23440972150212017	0.9563460841460147
>10	1.4223386183726372	12.1616513726774
>50	0.13559551050884572	4.252518271098156
>100	0.07116842663618082	5.848941573992357
>500	0.004396053186613863	1.35211979273889
>1k	0.004768600066801158	4.037309728836378
>5k	7.45093760437681E-4	2.409206452231788
>10k+	3.725468802188405E-4	3.585792507882285
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29211	0.9759215226928232	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26542	0.8867518761874948	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	24067	0.8040636502224564	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16046	0.5360869793272753	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10298	0.3440498387830164	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9500	0.3173891501688343	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9350	0.31237774253458955	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8855	0.2958400973415819	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8420	0.28130701520227214	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6718	0.22444424324570833	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6210	0.20747227605773275	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6083	0.20322928426073886	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5848	0.19537807896708873	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5283	0.17650177687810018	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5062	0.16911830296364624	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4562	0.1524136108494971	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3924	0.13109842371184272	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3691	0.1233140371866492	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3618	0.12087515213798344	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3368	0.11252280608090885	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3232	0.10797912982586026	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3147	0.10513933216645491	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3052	0.10196544066476657	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3006	0.10042860899026483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.340938422829835E-5	0.0
3	0.0	0.0	0.0	6.68187684565967E-5	0.0
4	0.0	0.0	0.0	6.68187684565967E-5	0.0
5	3.340938422829835E-5	0.0	0.0	1.0022815268489504E-4	0.0
6	3.340938422829835E-5	0.0	0.0	1.0022815268489504E-4	0.0
7	3.340938422829835E-5	0.0	0.0	1.336375369131934E-4	0.0
8	3.340938422829835E-5	0.0	0.0	1.336375369131934E-4	0.0
9	3.340938422829835E-5	0.0	0.0	1.6704692114149174E-4	0.0
10	3.340938422829835E-5	0.0	0.0	2.3386568959808844E-4	0.0
11	3.340938422829835E-5	0.0	0.0	3.006844580546852E-4	0.0
12	3.340938422829835E-5	0.0	0.0	4.3432199496787853E-4	0.0
13	3.340938422829835E-5	0.0	0.0	5.011407634244753E-4	0.0
14	3.340938422829835E-5	0.0	0.0	6.68187684565967E-4	0.0
15	3.340938422829835E-5	0.0	0.0	9.354627583923538E-4	0.0
16	6.68187684565967E-5	0.0	0.0	0.0017038785956432158	0.0
17	6.68187684565967E-5	0.0	0.0	0.0026393413540355694	0.0
18	6.68187684565967E-5	3.340938422829835E-5	0.0	0.0029400258120902546	0.0
19	1.0022815268489504E-4	3.340938422829835E-5	0.0	0.0035748041124279233	0.0
20	1.0022815268489504E-4	3.340938422829835E-5	0.0	0.004810951328874963	0.0
21	1.336375369131934E-4	3.340938422829835E-5	0.0	0.006514829924518178	0.0
22	1.336375369131934E-4	3.340938422829835E-5	0.0	0.010590774800370576	0.0
23	1.336375369131934E-4	1.6704692114149174E-4	0.0	0.017706973640998127	0.0
24	1.336375369131934E-4	1.6704692114149174E-4	0.0	0.029233211199761057	0.0
25	1.336375369131934E-4	2.004563053697901E-4	0.0	0.03364324991789644	0.0
26	1.6704692114149174E-4	2.004563053697901E-4	0.0	0.04095990506389378	0.0
27	1.6704692114149174E-4	2.004563053697901E-4	0.0	0.09678698610938032	0.0
28	1.6704692114149174E-4	2.004563053697901E-4	0.0	0.15702410587300225	0.0
29	1.6704692114149174E-4	2.004563053697901E-4	0.0	0.22297423033966318	0.0
30	1.6704692114149174E-4	2.3386568959808844E-4	0.0	0.3476914616639009	0.0
31	2.3386568959808844E-4	2.3386568959808844E-4	0.0	0.5169434021644603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6660	0.0	29.583334	1
GTATTGG	1090	0.0	20.876146	1
ATTGGAC	1090	0.0	20.70642	3
TTGGACC	1765	0.0	17.818697	4
GTATCAA	11670	0.0	17.77078	2
GGACCCT	1870	0.0	16.917112	6
TGGACCC	1890	0.0	16.542328	5
TATTGGA	1450	0.0	16.45862	2
GCGTAAT	80	3.3848334E-4	16.1875	11
GACCCTC	1965	0.0	16.00509	7
TGCGTAA	95	7.065729E-5	15.578946	10
CGTCTGT	560	0.0	14.535715	34
GTTATAC	180	3.3378456E-9	14.388887	3
ACCCTCG	2155	0.0	14.336426	8
TAAACGT	230	4.0017767E-11	13.673913	4
TATACTG	465	0.0	13.526881	5
CTAGTAC	370	0.0	13.5	3
CCCTCGC	2255	0.0	13.372506	9
TTATACT	735	0.0	13.340136	4
TTTAGCG	125	4.8494563E-5	13.320001	26
>>END_MODULE