FastQCFastQC Report
Wed 25 May 2016
SRR2088275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2545328
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT419471.647999786275089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT377171.4818129529868056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT336931.3237193791919941No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG205700.8081473193238751No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138770.5451949611209244No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT127740.5018606639301496No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA121320.47663798143107683No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT117210.460490750111577No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA115650.454361873990307No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA93310.3665932249203246No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT89080.34997454159149627No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT87610.34419925447722255No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG83590.3284056121647191No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72990.2867606846740381No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA71820.28216402758308556No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA62550.24574435986246174No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT60440.23745466203176957No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52700.2070460074300837No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA52390.20582808973931846No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC50480.19832414525750708No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45450.17856244853315564No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG41830.16434031291841367No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG41790.1641831622486375No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39740.15612919042260956No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39570.15546130007606093No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.1387640414123445No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC32920.12933500122577524No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG32470.1275670561907935No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA31770.12481691946971077No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31330.12308826210217308No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC31330.12308826210217308No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA30280.11896305702054902No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC30170.11853089267866461No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA29650.1164879339715746No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC28200.110791222192189No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG27290.10721604445478147No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG26980.10599812676401626No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC26630.10462305840347491No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC26370.10360157904992992No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT26020.10222651068938855No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA25880.10167648334517203No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA25850.10155862034283991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94750.029.560951
GTATTGG6250.021.0161
GCGGGTA555.145453E-420.18181823
AGTATCA5550.019.6666661
CGCGCTT500.007037172618.517
GTATCAA159750.018.2510192
ATTGGAC7300.017.993153
CGTCTGT10150.017.8620734
AACAACG4150.017.8313262
TTTTTAC20850.017.3908861
CGAACAC1401.873559E-917.17857232
TCGACCC650.001580646817.0769238
TATTGGA7800.017.0769212
TATACCG1554.0199666E-1016.7096775
TTGGACC16850.016.5786344
TGGACCC17250.016.5159425
GGACCCT16900.016.4201186
ACAACGC4200.016.2976193
CCGTGGT1101.4531672E-515.1363649
TAACAAC4700.014.9574461
Produced by FastQC (version 0.11.5)