FastQCFastQC Report
Wed 25 May 2016
SRR2088274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915493
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT153480.8012558646781794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141900.7408014542470267No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117070.6111742512240974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53550.27956249383318027No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG46470.24260072994263096No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT30090.15708749653483461No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT28320.14784705556219732No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25150.13129779122137225No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT24270.12670367367565424No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA22710.11855955620824508No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT21110.11020661521603055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.026.6173481
ACGTTTA1202.382876E-1020.04166626
TAAGCGC500.00703655318.514
CTGTGCG1601.8189894E-1218.59
CGATGCG650.001580453717.07692333
TAGTGCG650.001580453717.0769237
GTATCAA64500.016.8077532
CAATGCG1001.0939683E-414.819
AGCCGTC1252.9610546E-614.79999912
ATCGCTA750.00410573514.79999925
GTATATA3550.014.5915491
CGGACCA1152.2112548E-514.4782619
CGAATTA908.277563E-414.38888915
GCCGTCA1304.4473963E-614.23076913
TCACGTT1702.4392648E-814.14705924
GCGTTAT1051.6565403E-414.0952381
CGCGATA800.00630064313.87514
TACCGTG1356.571967E-613.7037047
TACATCG950.001245604113.6315795
ACGGACC1102.4577012E-413.4545458
Produced by FastQC (version 0.11.5)