##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1915493
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.946589207060534	31.0	30.0	33.0	27.0	34.0
2	30.804152769026043	31.0	31.0	34.0	27.0	34.0
3	30.83047601844538	31.0	31.0	34.0	27.0	34.0
4	35.00533335282353	35.0	35.0	37.0	32.0	37.0
5	34.87413266454119	35.0	35.0	37.0	32.0	37.0
6	34.969173471268235	35.0	35.0	37.0	32.0	37.0
7	34.60444908960774	35.0	35.0	37.0	30.0	37.0
8	34.79699064418403	35.0	35.0	37.0	32.0	37.0
9	36.27883213355517	38.0	35.0	39.0	32.0	39.0
10	36.163420069924555	38.0	35.0	39.0	32.0	39.0
11	36.249526884201615	38.0	35.0	39.0	32.0	39.0
12	35.989439794350595	38.0	35.0	39.0	30.0	39.0
13	36.15718773182674	38.0	35.0	39.0	32.0	39.0
14	37.104242093288775	39.0	36.0	40.0	31.0	41.0
15	37.14863275407428	39.0	36.0	40.0	31.0	41.0
16	37.08311802757828	39.0	36.0	40.0	31.0	41.0
17	37.11964543853723	39.0	36.0	40.0	31.0	41.0
18	37.10450468887122	39.0	36.0	40.0	31.0	41.0
19	37.18068925336715	39.0	36.0	40.0	31.0	41.0
20	37.13462539408915	39.0	36.0	40.0	31.0	41.0
21	37.077548182112906	39.0	36.0	40.0	31.0	41.0
22	36.9967319118368	39.0	36.0	40.0	31.0	41.0
23	36.882618208471655	39.0	35.0	40.0	31.0	41.0
24	36.80913059979859	39.0	35.0	40.0	30.0	41.0
25	36.71896477825813	38.0	35.0	40.0	30.0	41.0
26	36.48256140847291	38.0	35.0	40.0	30.0	41.0
27	36.34262667626559	38.0	35.0	40.0	30.0	41.0
28	36.21193134091328	38.0	35.0	40.0	30.0	41.0
29	36.06108610159369	38.0	34.0	40.0	29.0	41.0
30	35.92003364146984	38.0	34.0	40.0	29.0	41.0
31	35.72489014577448	38.0	34.0	40.0	28.0	41.0
32	35.47925155560475	38.0	34.0	40.0	27.0	41.0
33	35.65643727228447	38.0	34.0	40.0	28.0	41.0
34	35.68652195544437	38.0	34.0	40.0	28.0	41.0
35	35.6378739050469	38.0	34.0	40.0	28.0	41.0
36	35.576009413764496	38.0	34.0	40.0	27.0	41.0
37	35.43795513739805	38.0	34.0	40.0	27.0	41.0
38	35.351389955484045	38.0	34.0	40.0	27.0	41.0
39	35.36497549194907	38.0	34.0	40.0	26.0	41.0
40	35.28425789078843	38.0	34.0	40.0	26.0	41.0
41	35.16515643753331	38.0	34.0	40.0	25.0	41.0
42	35.03581897715105	38.0	33.0	40.0	25.0	41.0
43	34.714088226895115	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	6.0
16	18.0
17	44.0
18	178.0
19	351.0
20	659.0
21	1362.0
22	2654.0
23	4394.0
24	7503.0
25	11896.0
26	17840.0
27	26162.0
28	36622.0
29	49536.0
30	63878.0
31	79674.0
32	97853.0
33	119462.0
34	144355.0
35	173244.0
36	210231.0
37	258521.0
38	313878.0
39	295169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.95837781709461	24.826976658228457	13.199212944135008	23.015432580541926
2	18.073571660141802	23.464977423566673	35.248105840115315	23.21334507617621
3	19.19171722371212	26.500853827187047	31.816613268751176	22.490815680349655
4	13.622759258321487	17.02919300670898	36.5910499281386	32.75699780683093
5	12.176917378450353	39.45616089434939	35.21500731143366	13.151914415766594
6	31.367642690419643	37.63986608147354	15.999014352962918	14.993476875143893
7	24.968715625690095	34.04971983713853	22.893792877342804	18.087771659828565
8	26.260758979542082	34.64434482402181	20.709551013759906	18.38534518267621
9	25.289886206840745	14.24667174455871	22.914570818060938	37.5488712305396
10	15.857066561976474	28.291776581799045	34.96880437568814	20.882352480536344
11	32.47988898941421	23.059181108988653	23.978892118112675	20.48203778348446
12	19.654417948799605	27.66123394864925	31.871116208725375	20.81323189382577
13	29.50780817262188	21.841896577016986	27.613517773231226	21.03677747712991
14	20.778410571064473	23.11592890185451	28.286242758391705	27.819417768689313
15	23.182752429792224	29.919242722369642	25.292914147950423	21.605090699887704
16	21.9786759857645	28.6754898086289	27.04985087390035	22.29598333170625
17	20.3199907282355	28.382510403326975	28.17911629016655	23.11838257827097
18	20.69112233769583	27.41346483646769	30.643808147563057	21.251604678273424
19	20.755492189217083	26.746012645308543	31.690170624481528	20.80832454099284
20	22.7923568501686	26.137135452857308	30.708177999084306	20.362329697889788
21	22.510288474037754	26.64144426526226	29.96544492723283	20.882822333467153
22	21.96901789774225	26.590230295803746	30.407367711602184	21.03338409485182
23	21.414434821740407	27.309992779926628	30.581891972458266	20.693680425874696
24	20.5963686633154	27.254915575259215	30.054090513512712	22.094625247912678
25	21.527512760422514	27.44468395342609	30.452943445890952	20.574859840260444
26	21.707100991755123	27.11495160775842	29.868759635247947	21.309187765238505
27	21.302975265375544	27.704147182996753	29.759440520012344	21.23343703161536
28	21.04001424176439	27.694906742024116	29.910628751971423	21.354450264240068
29	20.53252087060616	27.504929540332434	30.399745652946784	21.56280393611462
30	20.399343667661537	27.85235967972736	31.01024122771527	20.738055424895833
31	21.141606886582203	27.598221450039233	30.32895447803777	20.9312171853408
32	21.28204070701381	27.889843502429922	29.71078463873269	21.117331151823578
33	21.770739960939558	27.63090233167127	29.405641263110855	21.192716444278314
34	21.16181056260712	28.341111139534313	29.70629493294938	20.790783364909192
35	20.614170868805054	28.751971424588866	29.81644934228421	20.817408364321874
36	20.908507627018214	27.565958215456803	30.475183151282724	21.050351006242256
37	20.866795127938342	27.264417045637863	30.291157420048	21.5776304063758
38	20.930695126528782	27.371282484457005	30.438221387392172	21.259801001622037
39	21.256668648749958	26.944708229160845	30.793377997204896	21.005245124884297
40	20.50965469463997	27.029386168469422	30.617600795199984	21.843358341690625
41	20.739517189569472	26.688011911293856	30.660775058953494	21.911695840183178
42	19.539930451325063	27.569403803616094	30.69319491117952	22.197470833879322
43	19.854470885563142	27.081487637908364	30.706559616767066	22.357481859761428
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	275.0
1	329.5
2	384.0
3	842.5
4	1301.0
5	1301.0
6	2118.0
7	2935.0
8	3123.0
9	3311.0
10	4987.5
11	6664.0
12	6664.0
13	11638.5
14	16613.0
15	25530.0
16	34447.0
17	34059.0
18	33671.0
19	33671.0
20	38129.0
21	42587.0
22	38397.0
23	34207.0
24	39578.5
25	44950.0
26	44950.0
27	51120.5
28	57291.0
29	62132.0
30	66973.0
31	72306.5
32	77640.0
33	77640.0
34	85531.5
35	93423.0
36	99378.5
37	105334.0
38	109044.5
39	112755.0
40	112755.0
41	116181.0
42	119607.0
43	125175.5
44	130744.0
45	143064.0
46	155384.0
47	155384.0
48	155949.5
49	156515.0
50	144322.5
51	132130.0
52	123650.5
53	115171.0
54	115171.0
55	105029.0
56	94887.0
57	84547.5
58	74208.0
59	66724.5
60	59241.0
61	59241.0
62	52146.5
63	45052.0
64	39130.0
65	33208.0
66	28000.5
67	22793.0
68	22793.0
69	19409.0
70	16025.0
71	13307.5
72	10590.0
73	8561.0
74	6532.0
75	6532.0
76	5140.5
77	3749.0
78	2964.0
79	2179.0
80	1730.0
81	1281.0
82	1281.0
83	986.0
84	691.0
85	521.0
86	351.0
87	288.0
88	225.0
89	225.0
90	174.0
91	123.0
92	76.0
93	29.0
94	22.5
95	16.0
96	16.0
97	8.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1915493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.15282176929935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.389521311579	36.806915820738176
2	14.234704552224308	14.278212006926797
3	5.38581572587338	8.103415685460517
4	2.4619623855392274	4.938974428986717
5	1.3102052151101025	3.285524431731174
6	0.7756715850381114	2.3341271253555798
7	0.5051296120740867	1.7733572783322893
8	0.32668813669903646	1.310746551520902
9	0.2314443438907912	1.044682823580055
>10	1.2025393425695405	11.212070025479862
>50	0.11367400397748414	3.9637494949179377
>100	0.05634878012541753	5.155640672516181
>500	0.003986836689712633	1.4244411833449855
>1k	0.001888501589863879	1.9161104389016896
>5k	1.0491675499243772E-4	0.2817732088512577
>10k+	3.1475026497731313E-4	2.1702588233557654
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15348	0.8012558646781794	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14190	0.7408014542470267	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11707	0.6111742512240974	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5355	0.27956249383318027	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4647	0.24260072994263096	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3009	0.15708749653483461	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2832	0.14784705556219732	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2515	0.13129779122137225	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2427	0.12670367367565424	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2271	0.11855955620824508	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2111	0.11020661521603055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0441176240268172E-4	0.0
3	0.0	0.0	0.0	1.5661764360402257E-4	0.0
4	0.0	0.0	0.0	2.0882352480536344E-4	0.0
5	0.0	0.0	0.0	2.0882352480536344E-4	0.0
6	0.0	0.0	0.0	2.0882352480536344E-4	0.0
7	0.0	0.0	0.0	2.610294060067043E-4	0.0
8	0.0	0.0	0.0	2.610294060067043E-4	0.0
9	0.0	0.0	0.0	3.1323528720804514E-4	0.0
10	0.0	0.0	0.0	3.1323528720804514E-4	0.0
11	0.0	0.0	0.0	4.698529308120677E-4	0.0
12	0.0	1.0441176240268172E-4	0.0	5.220588120134086E-4	0.0
13	0.0	1.0441176240268172E-4	0.0	6.786764556174312E-4	0.0
14	0.0	1.0441176240268172E-4	0.0	7.30882336818772E-4	0.0
15	0.0	1.0441176240268172E-4	0.0	7.30882336818772E-4	0.0
16	0.0	1.0441176240268172E-4	0.0	9.397058616241354E-4	0.0
17	0.0	1.0441176240268172E-4	0.0	0.0010963235052281579	0.0
18	0.0	1.0441176240268172E-4	0.0	0.0010963235052281579	0.0
19	0.0	1.0441176240268172E-4	0.0	0.0011485293864294988	0.0
20	0.0	1.0441176240268172E-4	0.0	0.0015139705548388848	0.0
21	0.0	1.0441176240268172E-4	0.0	0.001774999960845589	0.0
22	0.0	1.0441176240268172E-4	0.0	0.002819117584872406	0.0
23	0.0	1.0441176240268172E-4	0.0	0.00548161752614079	0.0
24	0.0	1.0441176240268172E-4	0.0	0.010232352715462807	0.0
25	0.0	1.0441176240268172E-4	0.0	0.013625734993549963	0.0
26	0.0	1.0441176240268172E-4	0.0	0.02129999953014707	0.0
27	0.0	1.0441176240268172E-4	0.0	0.06661470441291094	0.0
28	0.0	1.0441176240268172E-4	0.0	0.1144874974745405	0.0
29	0.0	1.0441176240268172E-4	0.0	0.17066102564718325	0.0
30	0.0	1.0441176240268172E-4	0.0	0.2587845531150466	0.0
31	0.0	1.0441176240268172E-4	0.0	0.41028602036133777	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3920	0.0	26.617348	1
ACGTTTA	120	2.382876E-10	20.041666	26
TAAGCGC	50	0.007036553	18.5	14
CTGTGCG	160	1.8189894E-12	18.5	9
CGATGCG	65	0.0015804537	17.076923	33
TAGTGCG	65	0.0015804537	17.076923	7
GTATCAA	6450	0.0	16.807753	2
CAATGCG	100	1.0939683E-4	14.8	19
AGCCGTC	125	2.9610546E-6	14.799999	12
ATCGCTA	75	0.004105735	14.799999	25
GTATATA	355	0.0	14.591549	1
CGGACCA	115	2.2112548E-5	14.478261	9
CGAATTA	90	8.277563E-4	14.388889	15
GCCGTCA	130	4.4473963E-6	14.230769	13
TCACGTT	170	2.4392648E-8	14.147059	24
GCGTTAT	105	1.6565403E-4	14.095238	1
CGCGATA	80	0.006300643	13.875	14
TACCGTG	135	6.571967E-6	13.703704	7
TACATCG	95	0.0012456041	13.631579	5
ACGGACC	110	2.4577012E-4	13.454545	8
>>END_MODULE