FastQCFastQC Report
Wed 25 May 2016
SRR2088273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970004
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT387481.3046447075492154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT361911.2185505474066702No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320591.079426155655009No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG193570.6517499639731126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.4396290375366498No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA110990.3737032003997301No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT107420.3616830145683305No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107020.36033621503540064No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT106730.3593597853740264No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA88030.2963969072095526No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT80840.27218818560513725No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT79310.2670366773916803No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG74420.250572053101612No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA63030.21222193640143247No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA62350.2099323771954516No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57020.19198627341916039No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT55210.18589200553265248No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA46920.15797958521267985No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC46130.15531965613514326No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.15481460631029453No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38480.1295621150678585No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38320.1290233952546865No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG38260.12882137532474702No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34490.11612778972688252No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34440.11595943978526628No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32960.11097628151342558No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC30990.1043432938137457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.029.5111481
GTATTGG10000.022.1999991
ATTGGAC10850.020.6313363
TATTGGA12000.018.8083342
GTATGCG500.007037443618.49999833
TTGGACC18350.018.4495934
ATCACGT2050.018.0487823
GCGCATA1258.594725E-917.7626
GTATCAA152700.017.4944342
TGGACCC19650.016.8524175
GGACCCT19600.016.6122446
CGTCTGT10150.016.4039434
ACGTTTA2200.015.97727326
TCACGTT2200.015.97727324
GACCCTC21050.015.2921617
TACGACG1353.9780025E-715.0740755
TATTCGC750.004106442414.810
TGCGACG1252.9623825E-614.79999922
TAGGTCG1152.2120654E-514.47826121
CTAGGCG1551.2121018E-714.32258122
Produced by FastQC (version 0.11.5)