##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2970004
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.852410973183876	31.0	30.0	33.0	27.0	34.0
2	30.729424606835547	31.0	31.0	34.0	27.0	34.0
3	30.732659619313644	31.0	31.0	34.0	27.0	34.0
4	34.94090411999445	35.0	35.0	37.0	32.0	37.0
5	34.85451602085384	35.0	35.0	37.0	32.0	37.0
6	34.96458422278219	35.0	35.0	37.0	32.0	37.0
7	34.577648716971424	35.0	35.0	37.0	30.0	37.0
8	34.800588484055915	35.0	35.0	37.0	32.0	37.0
9	36.20501251850166	38.0	35.0	39.0	32.0	39.0
10	36.143164790350454	38.0	35.0	39.0	31.0	39.0
11	36.232873087039614	38.0	35.0	39.0	32.0	39.0
12	35.971358961132715	38.0	35.0	39.0	30.0	39.0
13	36.137705201743834	38.0	35.0	39.0	31.0	39.0
14	37.02625080639622	39.0	36.0	40.0	31.0	41.0
15	37.07193458325309	39.0	36.0	40.0	31.0	41.0
16	36.99178889994761	38.0	36.0	40.0	31.0	41.0
17	37.0965207454266	39.0	36.0	40.0	31.0	41.0
18	37.10636921701116	39.0	36.0	40.0	31.0	41.0
19	37.19130243595632	39.0	36.0	40.0	31.0	41.0
20	37.15447083572951	39.0	36.0	40.0	31.0	41.0
21	37.08195578187774	39.0	36.0	40.0	31.0	41.0
22	37.00207811167931	39.0	36.0	40.0	31.0	41.0
23	36.87336515371697	39.0	35.0	40.0	31.0	41.0
24	36.802490838396174	39.0	35.0	40.0	30.0	41.0
25	36.70747783504669	38.0	35.0	40.0	30.0	41.0
26	36.4390616982334	38.0	35.0	40.0	30.0	41.0
27	36.29076358146319	38.0	35.0	40.0	30.0	41.0
28	36.13013282136994	38.0	35.0	40.0	30.0	41.0
29	35.964688936445874	38.0	34.0	40.0	29.0	41.0
30	35.80196861687728	38.0	34.0	40.0	29.0	41.0
31	35.5324299226533	38.0	34.0	40.0	27.0	41.0
32	35.289396243237384	38.0	34.0	40.0	27.0	41.0
33	35.39715939776512	38.0	34.0	40.0	27.0	41.0
34	35.37595605931844	38.0	34.0	40.0	27.0	41.0
35	35.277115788396245	38.0	34.0	40.0	27.0	41.0
36	35.192644184990996	38.0	34.0	40.0	26.0	41.0
37	35.03031612078637	38.0	33.0	40.0	25.0	41.0
38	34.89785535642376	38.0	33.0	40.0	25.0	41.0
39	34.850317373309935	38.0	33.0	40.0	25.0	41.0
40	34.68411894394755	38.0	33.0	40.0	24.0	41.0
41	34.5160444228358	38.0	33.0	40.0	23.0	41.0
42	34.32151943229706	38.0	33.0	40.0	23.0	41.0
43	33.973469396000816	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	7.0
15	10.0
16	41.0
17	100.0
18	270.0
19	606.0
20	1288.0
21	2583.0
22	4660.0
23	8030.0
24	13214.0
25	21054.0
26	31107.0
27	44440.0
28	61841.0
29	82551.0
30	105926.0
31	129703.0
32	156658.0
33	189897.0
34	226627.0
35	269615.0
36	324858.0
37	404648.0
38	460695.0
39	429572.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.57166387654697	25.008013457220933	13.532237667019977	21.888084999212122
2	18.053881408913927	23.043638998465994	35.874227778817804	23.02825181380227
3	19.890107892110585	26.473297679060366	32.052313734257595	21.584280694571454
4	13.06472987915168	15.835500558248405	35.020323204951914	36.079446357648
5	11.192846878320703	40.64105637568165	34.59126654374876	13.574830202248886
6	31.882313963213516	35.78129861104564	15.28230265009744	17.0540847756434
7	24.42134758067666	35.42954824303267	21.30000498315827	18.8490991931324
8	28.00258854870229	33.49099866532166	20.011420860039244	18.494991925936798
9	25.271413775873704	13.602001882825748	23.654715616544625	37.47186872475592
10	16.50044915764423	27.85420491016174	33.50779998949496	22.137545942699067
11	31.522920507851165	21.6415533447093	25.131380294437317	21.704145853002217
12	20.250073737274427	27.43359941602772	31.692280549117108	20.624046297580744
13	31.296860206248883	20.799130236861636	28.783732277801644	19.120277279087837
14	20.96192463040454	22.5481851202894	28.484574431549586	28.005315817756475
15	23.147712932373153	28.973428992014828	26.793398258049482	21.085459817562537
16	21.700576834239953	28.40666207856959	27.209087933888306	22.68367315330215
17	19.393879604202553	27.24100708281874	27.902757033323862	25.46235627965484
18	20.51815418430413	26.36181634772209	32.41258260931635	20.70744685865743
19	19.84094970915864	24.850606261809748	33.75396127412623	21.55448275490538
20	24.33791334961165	23.65198834749044	32.538845065528534	19.471253237369375
21	23.660843554419454	24.59542142030785	31.265143077248382	20.478591948024313
22	21.950711177493364	24.251246799667612	32.07568070615393	21.722361316685095
23	20.97956770428592	26.338550385790725	33.18200918247921	19.49987272744414
24	19.576842320751084	25.505723224615185	31.546792529572347	23.37064192506138
25	20.742665666443546	26.388314628532488	32.77200973466702	20.09700997035694
26	22.323067578360163	25.336935573150743	31.242348495153543	21.09764835333555
27	20.878860769211087	26.84794363913315	30.888375907911236	21.38481968374453
28	20.614180991002033	26.267439370452028	31.068274655522348	22.05010498302359
29	19.65360989412809	26.175621312294528	31.959519246438727	22.211249547138657
30	18.943947550238992	26.524408721335057	34.01463432372482	20.517009404701138
31	20.473945489635703	25.89077321107985	32.810460861332174	20.824820437952273
32	21.62683955981204	26.112759444095023	30.74669259704701	21.513708399045928
33	22.53108076622119	26.076227506764297	30.213730351878315	21.178961375136197
34	20.700409831097872	28.810668268460244	30.305817769942394	20.183104130499487
35	19.397078253093262	29.703899388687695	30.55120464484223	20.347817713376816
36	20.450208147867816	26.025183804466256	32.62473720574114	20.89987084192479
37	20.47131923054649	26.39363448668756	31.70908187329041	21.425964409475544
38	20.89020755527602	25.888315301932252	32.19278492554219	21.02869221724954
39	21.748724917542198	25.374410270154517	32.160529076728515	20.716335735574766
40	19.963306446725323	26.10430827702589	31.46046267951154	22.471922596737244
41	20.946739465670753	25.67525161582274	31.259116149338517	22.11889276916799
42	18.515496948825657	27.234475105084034	31.758980795985458	22.491047150104848
43	19.08967125970201	27.14592303579389	31.354233866351695	22.410171838152408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	249.0
1	376.0
2	503.0
3	1259.0
4	2015.0
5	2015.0
6	3615.5
7	5216.0
8	5873.0
9	6530.0
10	9834.5
11	13139.0
12	13139.0
13	24141.5
14	35144.0
15	57759.5
16	80375.0
17	77543.0
18	74711.0
19	74711.0
20	79332.0
21	83953.0
22	67232.0
23	50511.0
24	55341.5
25	60172.0
26	60172.0
27	65173.0
28	70174.0
29	73773.5
30	77373.0
31	83104.0
32	88835.0
33	88835.0
34	98002.5
35	107170.0
36	115668.5
37	124167.0
38	129463.0
39	134759.0
40	134759.0
41	143556.5
42	152354.0
43	174697.0
44	197040.0
45	247511.0
46	297982.0
47	297982.0
48	303079.0
49	308176.0
50	279911.5
51	251647.0
52	233236.5
53	214826.0
54	214826.0
55	187425.0
56	160024.0
57	131394.5
58	102765.0
59	90199.0
60	77633.0
61	77633.0
62	68486.0
63	59339.0
64	51995.0
65	44651.0
66	37969.5
67	31288.0
68	31288.0
69	26713.5
70	22139.0
71	18369.5
72	14600.0
73	11775.0
74	8950.0
75	8950.0
76	7099.0
77	5248.0
78	4088.0
79	2928.0
80	2307.0
81	1686.0
82	1686.0
83	1256.5
84	827.0
85	628.5
86	430.0
87	356.5
88	283.0
89	283.0
90	216.0
91	149.0
92	88.0
93	27.0
94	19.5
95	12.0
96	12.0
97	8.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2970004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.85840705733357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.70020860491744	28.578561006705307
2	14.092032137371616	11.233719063927685
3	5.629326088135538	6.731279120381206
4	2.762618802415279	4.404543390836463
5	1.568376887104384	3.125650219276012
6	0.9640372803255646	2.305499420259669
7	0.6313878678868655	1.761628025450765
8	0.42299847446645533	1.3488036303932078
9	0.3245284024914406	1.1641666651353024
>10	1.6384318908080053	12.196598259107834
>50	0.15632572078479542	4.349333954916164
>100	0.09411781674452899	7.041799602916372
>500	0.007290397014240673	1.9846860686154626
>1k	0.006861550131046862	5.615203528174659
>5k	6.861550131046861E-4	1.915151119625654
>10k+	7.719243897427719E-4	6.243376924278287
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38748	1.3046447075492154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36191	1.2185505474066702	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32059	1.079426155655009	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19357	0.6517499639731126	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13057	0.4396290375366498	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11099	0.3737032003997301	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10742	0.3616830145683305	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10702	0.36033621503540064	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10673	0.3593597853740264	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8803	0.2963969072095526	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8084	0.27218818560513725	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7931	0.2670366773916803	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7442	0.250572053101612	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6303	0.21222193640143247	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6235	0.2099323771954516	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5702	0.19198627341916039	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5521	0.18589200553265248	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4692	0.15797958521267985	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4613	0.15531965613514326	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4598	0.15481460631029453	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3848	0.1295621150678585	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3832	0.1290233952546865	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3826	0.12882137532474702	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3449	0.11612778972688252	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3444	0.11595943978526628	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3296	0.11097628151342558	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3099	0.1043432938137457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.73399766464961E-5	0.0
3	0.0	0.0	0.0	6.73399766464961E-5	0.0
4	0.0	0.0	0.0	6.73399766464961E-5	0.0
5	0.0	0.0	0.0	6.73399766464961E-5	0.0
6	3.366998832324805E-5	0.0	0.0	6.73399766464961E-5	0.0
7	3.366998832324805E-5	0.0	0.0	6.73399766464961E-5	0.0
8	6.73399766464961E-5	0.0	0.0	6.73399766464961E-5	0.0
9	6.73399766464961E-5	0.0	0.0	1.0100996496974415E-4	0.0
10	6.73399766464961E-5	0.0	0.0	1.346799532929922E-4	0.0
11	6.73399766464961E-5	0.0	0.0	2.693599065859844E-4	0.0
12	6.73399766464961E-5	0.0	0.0	4.3770984820222465E-4	0.0
13	6.73399766464961E-5	0.0	0.0	4.713798365254727E-4	0.0
14	6.73399766464961E-5	0.0	0.0	7.07069754788209E-4	0.0
15	6.73399766464961E-5	0.0	0.0	0.0012121195796369298	0.0
16	6.73399766464961E-5	0.0	0.0	0.002188549241011123	0.0
17	6.73399766464961E-5	0.0	0.0	0.0031313089140620684	0.0
18	6.73399766464961E-5	0.0	0.0	0.0036363587389107895	0.0
19	6.73399766464961E-5	0.0	0.0	0.004444438458668743	0.0
20	6.73399766464961E-5	0.0	0.0	0.005555548073335928	0.0
21	6.73399766464961E-5	0.0	0.0	0.007979787232609788	0.0
22	6.73399766464961E-5	0.0	0.0	0.012895605527804003	0.0
23	6.73399766464961E-5	0.0	0.0	0.023030272013101667	0.0
24	6.73399766464961E-5	0.0	0.0	0.03774405691036106	0.0
25	6.73399766464961E-5	0.0	0.0	0.04521879431812213	0.0
26	6.73399766464961E-5	0.0	0.0	0.05404033125881312	0.0
27	1.0100996496974415E-4	0.0	0.0	0.12134663791698597	0.0
28	1.0100996496974415E-4	0.0	0.0	0.19201994340748363	0.0
29	1.0100996496974415E-4	0.0	0.0	0.27225552558178373	0.0
30	3.0302989490923245E-4	0.0	0.0	0.42774353165854323	0.0
31	3.0302989490923245E-4	0.0	0.0	0.6267331626489392	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8745	0.0	29.511148	1
GTATTGG	1000	0.0	22.199999	1
ATTGGAC	1085	0.0	20.631336	3
TATTGGA	1200	0.0	18.808334	2
GTATGCG	50	0.0070374436	18.499998	33
TTGGACC	1835	0.0	18.449593	4
ATCACGT	205	0.0	18.04878	23
GCGCATA	125	8.594725E-9	17.76	26
GTATCAA	15270	0.0	17.494434	2
TGGACCC	1965	0.0	16.852417	5
GGACCCT	1960	0.0	16.612244	6
CGTCTGT	1015	0.0	16.40394	34
ACGTTTA	220	0.0	15.977273	26
TCACGTT	220	0.0	15.977273	24
GACCCTC	2105	0.0	15.292161	7
TACGACG	135	3.9780025E-7	15.074075	5
TATTCGC	75	0.0041064424	14.8	10
TGCGACG	125	2.9623825E-6	14.799999	22
TAGGTCG	115	2.2120654E-5	14.478261	21
CTAGGCG	155	1.2121018E-7	14.322581	22
>>END_MODULE