FastQCFastQC Report
Wed 25 May 2016
SRR2088272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882611
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317471.1013279280485642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT299821.0400987160598498No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261570.907406514441248No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG154610.5363540207124721No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111830.3879468995296278No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96390.3343843480788771No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86910.30149749654046276No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT85150.2953919207274239No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT84480.29306763902586924No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA67470.23405863642371447No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT63920.2217434124826416No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT58590.20325323118519978No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG57800.2005126602236653No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49970.17334978600997497No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48060.16672384862196113No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46020.15964693120230236No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT40450.1403241713849007No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39310.1363694234150914No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36090.12519899493896333No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35390.12277064092241374No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34370.11923218221258436No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30180.10469674888495188No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29800.1033784995616821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79600.028.5634421
GTATTGG10800.020.8981481
ATTGGAC11000.020.5181833
GGTCCGT609.241425E-418.56
GTATCAA130850.017.9980892
TATTGGA13200.017.659092
TTGGACC17800.017.5646084
GAACCGT953.6102047E-617.5263166
GGACCCT17600.016.9232966
TGGACCC18850.015.8010625
ATAACGC1508.11433E-814.83
CGGTATA2101.364242E-1014.09523826
ACGCCTA1458.922616E-714.0344836
ACGGACC1458.922616E-714.0344838
TTGGACG800.006301660613.8754
GTCTAAC2304.0017767E-1113.6739131
GTACGAC950.001245870513.63157911
CGTCTGT7900.013.5822834
TCTAACG1501.3073404E-613.5666672
TATACAG4100.013.5365865
Produced by FastQC (version 0.11.5)