##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088272_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2882611
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.952019193710147	31.0	30.0	33.0	27.0	34.0
2	30.77407912479346	31.0	31.0	34.0	27.0	34.0
3	30.777646376843773	31.0	31.0	34.0	27.0	34.0
4	34.9543830228914	35.0	35.0	37.0	32.0	37.0
5	34.84779007642724	35.0	35.0	37.0	32.0	37.0
6	34.95371904152173	35.0	35.0	37.0	32.0	37.0
7	34.58258502447954	35.0	35.0	37.0	30.0	37.0
8	34.797169996229115	35.0	35.0	37.0	32.0	37.0
9	36.24099297477183	38.0	35.0	39.0	32.0	39.0
10	36.14894413432822	38.0	35.0	39.0	31.0	39.0
11	36.233927852214535	38.0	35.0	39.0	32.0	39.0
12	35.972584923876305	38.0	35.0	39.0	30.0	39.0
13	36.14502789311496	38.0	35.0	39.0	31.0	39.0
14	37.064179315211106	39.0	36.0	40.0	31.0	41.0
15	37.10222815357327	39.0	36.0	40.0	31.0	41.0
16	37.02618702280675	39.0	36.0	40.0	31.0	41.0
17	37.096285971294776	39.0	36.0	40.0	31.0	41.0
18	37.090121074262186	39.0	36.0	40.0	31.0	41.0
19	37.16622811749487	39.0	36.0	40.0	31.0	41.0
20	37.12406321907465	39.0	36.0	40.0	31.0	41.0
21	37.0577327291126	39.0	36.0	40.0	31.0	41.0
22	36.97140994744001	39.0	36.0	40.0	31.0	41.0
23	36.85482883399806	39.0	35.0	40.0	31.0	41.0
24	36.78475833194281	39.0	35.0	40.0	30.0	41.0
25	36.69227412231481	38.0	35.0	40.0	30.0	41.0
26	36.43285167509595	38.0	35.0	40.0	30.0	41.0
27	36.289674534649315	38.0	35.0	40.0	30.0	41.0
28	36.13142911062228	38.0	35.0	40.0	30.0	41.0
29	35.96724358576304	38.0	34.0	40.0	29.0	41.0
30	35.80775449757182	38.0	34.0	40.0	29.0	41.0
31	35.56585019622835	38.0	34.0	40.0	27.0	41.0
32	35.35299560016943	38.0	34.0	40.0	27.0	41.0
33	35.49081093494752	38.0	34.0	40.0	27.0	41.0
34	35.49301345204053	38.0	34.0	40.0	27.0	41.0
35	35.40657168102113	38.0	34.0	40.0	27.0	41.0
36	35.32354382884128	38.0	34.0	40.0	27.0	41.0
37	35.17474539575406	38.0	34.0	40.0	26.0	41.0
38	35.05326039482955	38.0	33.0	40.0	25.0	41.0
39	35.03492770963547	38.0	33.0	40.0	25.0	41.0
40	34.90056341282261	38.0	33.0	40.0	25.0	41.0
41	34.75038671537713	38.0	33.0	40.0	24.0	41.0
42	34.58275570307614	38.0	33.0	40.0	23.0	41.0
43	34.24752906306123	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	3.0
15	10.0
16	27.0
17	106.0
18	238.0
19	566.0
20	1221.0
21	2365.0
22	4373.0
23	7345.0
24	12407.0
25	19685.0
26	29349.0
27	42662.0
28	58740.0
29	78412.0
30	100442.0
31	124119.0
32	150091.0
33	181272.0
34	218288.0
35	259875.0
36	315301.0
37	391189.0
38	464114.0
39	420407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.66667718953407	24.81587005669513	13.236229238006795	22.281223515764008
2	18.167626502500685	23.18894224715024	35.639147980771604	23.004283269577478
3	19.910248035548328	26.688131003454853	31.64513005743751	21.756490903559307
4	13.385573010024594	16.135059499877023	35.70408910532847	34.77527838476992
5	11.534612197067172	40.34599188027798	34.80868559788331	13.310710324771534
6	31.83998812187978	36.495871277810295	15.481242526306879	16.18289807400305
7	24.736844478842272	34.70572338758161	21.935391213035683	18.622040920540442
8	27.429403412392446	33.994701331535886	20.271448350124246	18.304446905947422
9	25.32703857717881	13.63139181804274	23.118832197615287	37.92273740716316
10	16.05502788964588	27.803994364831052	34.171346740854034	21.96963100466903
11	32.00594877352511	21.77886645128323	24.799946992500896	21.415237782690763
12	20.069582749805644	27.169881749566628	31.962966907432182	20.797568593195546
13	30.754756711883775	20.991732842204517	28.448930500854953	19.804579945056755
14	20.865180907170615	22.352686505393894	28.223197649630837	28.558934937804647
15	23.07356074059247	29.0203915824924	26.444046734019956	21.462000942895173
16	21.8707623054238	28.428775162517596	26.94144301815264	22.75901951390597
17	19.749768525826063	27.08870534387054	28.043395380091173	25.11813075021222
18	20.777031656369868	26.55179627081143	31.76852513224989	20.90264694056881
19	20.220765132721688	25.171138249316332	32.88411096745277	21.72398565050921
20	24.059576543626594	24.336755809229896	31.738101325499695	19.865566321643815
21	23.450163757787646	24.96878697819442	30.625741732061663	20.955307531956272
22	22.018579683488337	24.700349787050698	31.513166362023874	21.76790416743709
23	21.209001145142373	26.433361976347136	32.30709936234892	20.05053751616156
24	20.27675603818899	25.61396594961998	30.928071807122087	23.181206205068946
25	21.130253093462837	26.45844340426093	31.784101288727477	20.62720221354876
26	22.366805649461547	25.606507433712007	30.60274175044777	21.423945166378676
27	21.146280229972064	26.834803586054452	30.40139651170415	21.617519672269342
28	20.950589587009834	26.37966066180973	30.562396383001385	22.10735336817906
29	20.21375759684536	26.244956395434553	31.36611218093596	22.17517382678412
30	19.698703709935195	26.565603197934095	32.8565318039791	20.87916128815161
31	20.8323981279472	26.002606664582906	32.05860936491257	21.10638584255732
32	21.700499998092006	26.255606462335706	30.2550708368212	21.78882270275108
33	22.51264565354118	26.105395421026284	29.982644206936005	21.39931471849653
34	20.997699654930894	28.415141689253247	29.987292770339113	20.599865885476742
35	20.006584308462017	29.310545196698413	30.090116217554154	20.59275427728542
36	20.853004446316202	26.038095323996195	31.92341942773409	21.185480801953506
37	20.9028550851988	26.358395218779084	31.110822792253273	21.62792690376884
38	21.111346622905415	25.9136248352622	31.668927926799697	21.30610061503269
39	21.755901160441002	25.46760558396537	31.755203875930537	21.02128937966309
40	20.366605136801326	26.11781471728235	31.091118433947557	22.424461711968767
41	21.184509460346888	25.603975007380463	30.87738858971953	22.334126942553123
42	19.067401047175633	27.117013013549172	31.306617507530497	22.50896843174469
43	19.63601748553655	26.873136888744263	30.920717363529103	22.570128262190075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	236.0
1	345.5
2	455.0
3	1208.0
4	1961.0
5	1961.0
6	3499.5
7	5038.0
8	5487.0
9	5936.0
10	8856.0
11	11776.0
12	11776.0
13	21123.0
14	30470.0
15	49286.5
16	68103.0
17	66004.0
18	63905.0
19	63905.0
20	68943.5
21	73982.0
22	62098.0
23	50214.0
24	55565.5
25	60917.0
26	60917.0
27	67606.0
28	74295.0
29	78838.5
30	83382.0
31	89465.5
32	95549.0
33	95549.0
34	103063.0
35	110577.0
36	119686.0
37	128795.0
38	134207.5
39	139620.0
40	139620.0
41	145753.0
42	151886.0
43	169623.5
44	187361.0
45	227531.0
46	267701.0
47	267701.0
48	272552.0
49	277403.0
50	253823.0
51	230243.0
52	217636.0
53	205029.0
54	205029.0
55	179014.0
56	152999.0
57	129906.0
58	106813.0
59	94584.0
60	82355.0
61	82355.0
62	72936.5
63	63518.0
64	55857.5
65	48197.0
66	41685.5
67	35174.0
68	35174.0
69	30107.0
70	25040.0
71	21519.5
72	17999.0
73	14659.5
74	11320.0
75	11320.0
76	8881.5
77	6443.0
78	5106.0
79	3769.0
80	2906.5
81	2044.0
82	2044.0
83	1554.0
84	1064.0
85	794.0
86	524.0
87	407.0
88	290.0
89	290.0
90	231.5
91	173.0
92	103.0
93	33.0
94	24.5
95	16.0
96	16.0
97	9.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2882611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.451921872169784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58427317109124	32.70962362021748
2	13.922521881789415	12.377657099057547
3	5.17290289321715	6.898364257848294
4	2.4549023552726084	4.365005108015343
5	1.3114056183612657	2.9147250045059745
6	0.7644793542544112	2.038954591692234
7	0.5311741976087256	1.6528199752830885
8	0.3554917985051096	1.2641834922677007
9	0.2539735228361539	1.01606500752412
>10	1.4271628625521173	11.857604459521905
>50	0.13254496192383683	4.104679826192975
>100	0.07781990925306077	6.452469675176513
>500	0.004728113889606339	1.4376234736464322
>1k	0.0055949347693592544	4.7941332776974175
>5k	6.304151852799159E-4	2.104224548264394
>10k+	3.940094907999475E-4	4.011866583088697
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31747	1.1013279280485642	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29982	1.0400987160598498	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26157	0.907406514441248	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15461	0.5363540207124721	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11183	0.3879468995296278	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9639	0.3343843480788771	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8691	0.30149749654046276	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8515	0.2953919207274239	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8448	0.29306763902586924	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6747	0.23405863642371447	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6392	0.2217434124826416	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5859	0.20325323118519978	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5780	0.2005126602236653	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4997	0.17334978600997497	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4806	0.16672384862196113	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4602	0.15964693120230236	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4045	0.1403241713849007	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3931	0.1363694234150914	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3609	0.12519899493896333	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3539	0.12277064092241374	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3437	0.11923218221258436	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3018	0.10469674888495188	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2980	0.1033784995616821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.469077166499399E-5	0.0	3.469077166499399E-5	0.0
5	0.0	6.938154332998798E-5	0.0	6.938154332998798E-5	0.0
6	3.469077166499399E-5	6.938154332998798E-5	0.0	6.938154332998798E-5	0.0
7	3.469077166499399E-5	6.938154332998798E-5	0.0	6.938154332998798E-5	0.0
8	6.938154332998798E-5	6.938154332998798E-5	0.0	1.0407231499498198E-4	0.0
9	6.938154332998798E-5	6.938154332998798E-5	0.0	1.7345385832496997E-4	0.0
10	6.938154332998798E-5	6.938154332998798E-5	0.0	2.0814462998996396E-4	0.0
11	6.938154332998798E-5	6.938154332998798E-5	0.0	4.509800316449219E-4	0.0
12	6.938154332998798E-5	6.938154332998798E-5	0.0	5.897431183048979E-4	0.0
13	6.938154332998798E-5	6.938154332998798E-5	0.0	6.591246616348858E-4	0.0
14	6.938154332998798E-5	6.938154332998798E-5	0.0	8.325785199598558E-4	0.0
15	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.0012488677799397838	0.0
16	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.0017692293549146938	0.0
17	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.0029140248198594953	0.0
18	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.0034343863948344054	0.0
19	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.004336346458124249	0.0
20	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.005203615749749099	0.0
21	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.007631969766298679	0.0
22	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.013494710177682664	0.0
23	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.022930600070561028	0.0
24	1.0407231499498198E-4	6.938154332998798E-5	0.0	0.03750072416985851	0.0
25	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.04412666155787236	0.0
26	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.05293811756078083	0.0
27	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.11819145906263454	0.0
28	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.18039201265796875	0.0
29	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.25251412694949127	0.0
30	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.38784282721463287	0.0
31	1.3876308665997596E-4	6.938154332998798E-5	0.0	0.5739241264256606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7960	0.0	28.563442	1
GTATTGG	1080	0.0	20.898148	1
ATTGGAC	1100	0.0	20.518183	3
GGTCCGT	60	9.241425E-4	18.5	6
GTATCAA	13085	0.0	17.998089	2
TATTGGA	1320	0.0	17.65909	2
TTGGACC	1780	0.0	17.564608	4
GAACCGT	95	3.6102047E-6	17.526316	6
GGACCCT	1760	0.0	16.923296	6
TGGACCC	1885	0.0	15.801062	5
ATAACGC	150	8.11433E-8	14.8	3
CGGTATA	210	1.364242E-10	14.095238	26
ACGCCTA	145	8.922616E-7	14.034483	6
ACGGACC	145	8.922616E-7	14.034483	8
TTGGACG	80	0.0063016606	13.875	4
GTCTAAC	230	4.0017767E-11	13.673913	1
GTACGAC	95	0.0012458705	13.631579	11
CGTCTGT	790	0.0	13.58228	34
TCTAACG	150	1.3073404E-6	13.566667	2
TATACAG	410	0.0	13.536586	5
>>END_MODULE