FastQCFastQC Report
Wed 25 May 2016
SRR2088271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603759
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324101.2447388563995363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304371.16896379426821No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT274981.0560885243219515No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG187750.7210728796328693No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109400.4201617738047185No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109380.4200849617802569No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA106310.40829431602540783No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT98470.37818400243647743No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT97820.3756876116414768No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA78790.302600970366305No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT72740.279365332966684No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT69850.2682659954319889No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67590.2595862366678329No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58650.2252512617335168No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57240.21983601400897704No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA54870.21073378911028248No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT46940.180277821411275No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA41130.15796392830519262No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC40150.1542001391065763No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40000.15362404892311463No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38930.14951460561442131No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35070.13468988489334074No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG34340.1318862460004939No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33170.12739274256949282No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC30200.11598615693695154No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29520.11337454810525859No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA28860.11083975129802721No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28390.1090346687231806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74500.029.500671
GTATTGG10500.025.0190471
ATTGGAC12050.022.8755193
TATTGGA13300.021.4210532
GACGTAT450.003827088320.55555728
TTGGACC18900.019.7724884
GGACCCT18950.018.8416886
TGGACCC19800.018.1262635
GTATCAA133600.017.1152692
CGAACGA650.001580660117.07692316
GACCCTC21100.016.3080587
AGCCGTC1606.311893E-1016.187512
TCTAACG1606.311893E-1016.18752
ACGTTTA1403.4784534E-815.85714326
CCGCGTT700.00259367515.85714311
ATAACGC1403.4784534E-815.8571433
TATACCG1902.7284841E-1115.5789485
CGTCTGT7050.015.48226934
CGGGATA2059.276846E-1114.43902524
AGTATCA5400.014.0462951
Produced by FastQC (version 0.11.5)