##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2603759
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.964915339706938	31.0	30.0	33.0	27.0	34.0
2	30.793461299605685	31.0	31.0	34.0	27.0	34.0
3	30.791254106082782	31.0	31.0	34.0	27.0	34.0
4	34.98036492624701	35.0	35.0	37.0	32.0	37.0
5	34.886857808268736	35.0	35.0	37.0	32.0	37.0
6	34.98523442453776	35.0	35.0	37.0	32.0	37.0
7	34.59849701911736	35.0	35.0	37.0	30.0	37.0
8	34.82360771484611	35.0	35.0	37.0	32.0	37.0
9	36.23870988059955	38.0	35.0	39.0	32.0	39.0
10	36.177879750007584	38.0	35.0	39.0	32.0	39.0
11	36.260468422768774	38.0	35.0	39.0	32.0	39.0
12	35.99032590957919	38.0	35.0	39.0	30.0	39.0
13	36.15463412704479	38.0	35.0	39.0	31.0	39.0
14	37.04970314072846	39.0	36.0	40.0	31.0	41.0
15	37.09353092970586	39.0	36.0	40.0	31.0	41.0
16	37.02402756937182	39.0	36.0	40.0	31.0	41.0
17	37.117232047973715	39.0	36.0	40.0	31.0	41.0
18	37.11983520748272	39.0	36.0	40.0	31.0	41.0
19	37.20582780510792	39.0	36.0	40.0	31.0	41.0
20	37.16696937005307	39.0	36.0	40.0	31.0	41.0
21	37.10369008806114	39.0	36.0	40.0	31.0	41.0
22	37.01778812862481	39.0	36.0	40.0	31.0	41.0
23	36.88429228665172	39.0	35.0	40.0	31.0	41.0
24	36.81010953778748	39.0	35.0	40.0	30.0	41.0
25	36.72418568692417	38.0	35.0	40.0	30.0	41.0
26	36.461648332276525	38.0	35.0	40.0	30.0	41.0
27	36.32022817779986	38.0	35.0	40.0	30.0	41.0
28	36.155975649052	38.0	35.0	40.0	30.0	41.0
29	35.993749805569564	38.0	34.0	40.0	29.0	41.0
30	35.82727587307428	38.0	34.0	40.0	29.0	41.0
31	35.563621287530836	38.0	34.0	40.0	27.0	41.0
32	35.33678616185292	38.0	34.0	40.0	27.0	41.0
33	35.45403049975055	38.0	34.0	40.0	27.0	41.0
34	35.44678098088187	38.0	34.0	40.0	27.0	41.0
35	35.349704024066746	38.0	34.0	40.0	27.0	41.0
36	35.25986506431663	38.0	34.0	40.0	26.0	41.0
37	35.10570486746277	38.0	33.0	40.0	26.0	41.0
38	34.98504892349868	38.0	33.0	40.0	25.0	41.0
39	34.936812124317186	38.0	33.0	40.0	25.0	41.0
40	34.77977531714725	38.0	33.0	40.0	24.0	41.0
41	34.62172919997588	38.0	33.0	40.0	24.0	41.0
42	34.41197745259834	38.0	33.0	40.0	23.0	41.0
43	34.07072121498188	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	3.0
15	8.0
16	33.0
17	72.0
18	205.0
19	485.0
20	1069.0
21	2040.0
22	3748.0
23	6717.0
24	10950.0
25	17427.0
26	26620.0
27	38630.0
28	53258.0
29	71646.0
30	91381.0
31	112430.0
32	135971.0
33	165243.0
34	198258.0
35	235085.0
36	285666.0
37	355975.0
38	409383.0
39	381451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.65247935772857	24.95269339443474	13.436612221023529	21.958215026813157
2	17.85752828890846	22.9678706823481	36.2399899529872	22.93461107575624
3	19.652049210391592	26.840003241467432	32.16453596511813	21.343411583022853
4	12.824535604101609	15.822201670738345	35.608556705900966	35.744706019259084
5	11.113202105110343	40.65433859278067	34.86724385782248	13.36521544428651
6	31.566247106587053	36.18372514506911	15.470709846802258	16.779317901541578
7	24.44139415360638	35.31501955442113	21.526454637314743	18.717131654657745
8	27.93975940169578	33.56378220872208	20.106584365142858	18.389874024439283
9	25.145683605894405	13.637667695051652	23.774051285084372	37.44259741396957
10	16.30239204165977	27.749342393055578	33.62745937700071	22.32080618828394
11	31.384855510821087	21.633184945304077	25.318741097006292	21.663218446868544
12	20.205134192527037	27.313126906138397	31.902376525630828	20.57936237570374
13	31.212642951978275	20.78003378960956	28.853400026653773	19.153923231758395
14	20.918449057689287	22.56341696754577	28.302004909056482	28.21612906570846
15	23.037731218595884	28.898911151147246	26.939129159035076	21.124228471221798
16	21.812118556287274	28.51323797632577	27.075662532515487	22.598980934871467
17	19.366001231296753	27.042825392058173	27.905923704920465	25.68524967172461
18	20.594340720473745	26.341992480870925	32.33724780211993	20.726418996535394
19	19.69594728237137	24.67202225705221	33.75535139772921	21.876679062847217
20	24.53195553044656	23.66397965403096	32.27099743102184	19.533067384500637
21	23.73433946843775	24.611187133678655	31.044040558285158	20.61043283959844
22	22.094825212318035	24.1950963971704	32.04574616928832	21.66433222122324
23	21.01576989268208	26.297979190854452	33.123226842422824	19.563024074040648
24	19.702591522487296	25.275611145271128	31.437394935552792	23.584402396688787
25	20.90320187083367	26.33988015019823	32.58807746799915	20.16884051096895
26	22.437214811355428	25.261055266635662	31.11866344004956	21.18306648195935
27	20.894291675996126	26.864160623160593	30.756072278578777	21.485475422264503
28	20.652909889125684	26.266486260825218	30.87048378901427	22.210120061034836
29	19.787507215529548	26.101916498416326	31.893005458646517	22.217570827407606
30	19.045003781071905	26.505794123035198	33.85282585677093	20.596376239121977
31	20.522444665577726	25.81878737625103	32.76697267297012	20.891795285201127
32	21.76895019853988	25.99791301729538	30.457580751521167	21.775556032643575
33	22.682590823497872	25.96184977181068	29.998168033216594	21.357391371474858
34	20.839831950652883	28.96120570298557	30.09656423655185	20.1023981098097
35	19.472078637078162	30.015604362769366	30.28463847844597	20.2276785217065
36	20.630980056141908	26.019343572120153	32.550477982025214	20.79919838971272
37	20.660821527645222	26.539783443859438	31.45310299455518	21.346292033940163
38	20.903585930955977	25.957701922489758	32.06775281429656	21.070959332257708
39	21.918042338019763	25.528207487712955	32.031881598873014	20.52186857539427
40	20.01352659750768	26.3704129299217	31.136253393651252	22.47980707891936
41	21.237871861412675	25.835301961510265	30.99741565943699	21.929410517640076
42	18.5519858020654	27.629362010846627	31.516127260625886	22.302524926462088
43	19.3131545584672	27.41593980088019	30.965269827199833	22.30563581345278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	163.0
1	279.0
2	395.0
3	1045.0
4	1695.0
5	1695.0
6	2953.0
7	4211.0
8	4735.5
9	5260.0
10	8057.5
11	10855.0
12	10855.0
13	20067.0
14	29279.0
15	48212.5
16	67146.0
17	65306.5
18	63467.0
19	63467.0
20	67829.0
21	72191.0
22	58667.0
23	45143.0
24	49711.5
25	54280.0
26	54280.0
27	59516.5
28	64753.0
29	67675.5
30	70598.0
31	75123.0
32	79648.0
33	79648.0
34	86487.0
35	93326.0
36	101446.5
37	109567.0
38	113977.0
39	118387.0
40	118387.0
41	124607.5
42	130828.0
43	150901.0
44	170974.0
45	215692.0
46	260410.0
47	260410.0
48	265876.0
49	271342.0
50	247417.5
51	223493.0
52	209561.0
53	195629.0
54	195629.0
55	167615.0
56	139601.0
57	115403.5
58	91206.0
59	79257.5
60	67309.0
61	67309.0
62	58905.5
63	50502.0
64	44291.0
65	38080.0
66	32678.5
67	27277.0
68	27277.0
69	22761.5
70	18246.0
71	15075.5
72	11905.0
73	9630.0
74	7355.0
75	7355.0
76	5716.5
77	4078.0
78	3256.5
79	2435.0
80	1858.5
81	1282.0
82	1282.0
83	963.5
84	645.0
85	508.5
86	372.0
87	296.0
88	220.0
89	220.0
90	189.5
91	159.0
92	94.5
93	30.0
94	21.5
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2603759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.59280079812012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73643457774803	29.525755995800218
2	14.055680326502271	11.411188631515252
3	5.429803147556395	6.612327526252872
4	2.542331971468418	4.12801501122038
5	1.3591794286778058	2.7586449898610446
6	0.8029344479716936	1.9556014860278101
7	0.5531607204923713	1.5718040055404003
8	0.3658072097123552	1.187931135549583
9	0.2887027603954458	1.054732827833976
>10	1.587131151834906	12.22990856829908
>50	0.16532944965723695	4.698963696070358
>100	0.09835132911463766	7.445804709887111
>500	0.006521750399088135	1.789265750049153
>1k	0.007097198963713513	5.562183635621341
>5k	8.631728469381299E-4	2.5540027358019963
>10k+	6.713566587296567E-4	5.513869294669385
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32410	1.2447388563995363	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30437	1.16896379426821	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27498	1.0560885243219515	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18775	0.7210728796328693	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10940	0.4201617738047185	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10938	0.4200849617802569	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10631	0.40829431602540783	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9847	0.37818400243647743	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9782	0.3756876116414768	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7879	0.302600970366305	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7274	0.279365332966684	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6985	0.2682659954319889	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6759	0.2595862366678329	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5865	0.2252512617335168	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5724	0.21983601400897704	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5487	0.21073378911028248	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4694	0.180277821411275	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4113	0.15796392830519262	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4015	0.1542001391065763	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4000	0.15362404892311463	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3893	0.14951460561442131	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3507	0.13468988489334074	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3434	0.1318862460004939	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3317	0.12739274256949282	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3020	0.11598615693695154	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2952	0.11337454810525859	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2886	0.11083975129802721	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2839	0.1090346687231806	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.840601223077866E-5	0.0	0.0	0.0
3	0.0	3.840601223077866E-5	0.0	0.0	0.0
4	0.0	3.840601223077866E-5	0.0	0.0	0.0
5	0.0	3.840601223077866E-5	0.0	0.0	0.0
6	7.681202446155731E-5	3.840601223077866E-5	0.0	0.0	0.0
7	7.681202446155731E-5	3.840601223077866E-5	0.0	0.0	0.0
8	7.681202446155731E-5	3.840601223077866E-5	0.0	0.0	0.0
9	7.681202446155731E-5	7.681202446155731E-5	0.0	1.1521803669233597E-4	0.0
10	7.681202446155731E-5	1.1521803669233597E-4	0.0	4.224661345385652E-4	0.0
11	7.681202446155731E-5	1.5362404892311463E-4	0.0	4.992781590001226E-4	0.0
12	7.681202446155731E-5	1.9203006115389327E-4	0.0	7.681202446155731E-4	0.0
13	1.1521803669233597E-4	1.9203006115389327E-4	0.0	8.065262568463517E-4	0.0
14	1.1521803669233597E-4	1.9203006115389327E-4	0.0	9.601503057694664E-4	0.0
15	1.1521803669233597E-4	1.9203006115389327E-4	0.0	0.0012673984036156956	0.0
16	1.1521803669233597E-4	1.9203006115389327E-4	0.0	0.0020739246604620475	0.0
17	1.1521803669233597E-4	1.9203006115389327E-4	0.0	0.0030724809784622924	0.0
18	1.1521803669233597E-4	2.688420856154506E-4	0.0	0.0037637891986163083	0.0
19	1.1521803669233597E-4	2.688420856154506E-4	0.0	0.004608721467693438	0.0
20	1.1521803669233597E-4	2.688420856154506E-4	0.0	0.006068149932463028	0.0
21	1.1521803669233597E-4	2.688420856154506E-4	0.0	0.008218886617386633	0.0
22	1.1521803669233597E-4	2.688420856154506E-4	0.0	0.01401819446423421	0.0
23	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.023619697521928874	0.0
24	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.038060358120701646	0.0
25	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.0433603878085491	0.0
26	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.052385800682782084	0.0
27	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.10661508995264155	0.0
28	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.1638784541887325	0.0
29	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.2348143587789807	0.0
30	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.36981149177016764	3.840601223077866E-5
31	1.1521803669233597E-4	3.0724809784622926E-4	0.0	0.5476697344109036	3.840601223077866E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7450	0.0	29.50067	1
GTATTGG	1050	0.0	25.019047	1
ATTGGAC	1205	0.0	22.875519	3
TATTGGA	1330	0.0	21.421053	2
GACGTAT	45	0.0038270883	20.555557	28
TTGGACC	1890	0.0	19.772488	4
GGACCCT	1895	0.0	18.841688	6
TGGACCC	1980	0.0	18.126263	5
GTATCAA	13360	0.0	17.115269	2
CGAACGA	65	0.0015806601	17.076923	16
GACCCTC	2110	0.0	16.308058	7
AGCCGTC	160	6.311893E-10	16.1875	12
TCTAACG	160	6.311893E-10	16.1875	2
ACGTTTA	140	3.4784534E-8	15.857143	26
CCGCGTT	70	0.002593675	15.857143	11
ATAACGC	140	3.4784534E-8	15.857143	3
TATACCG	190	2.7284841E-11	15.578948	5
CGTCTGT	705	0.0	15.482269	34
CGGGATA	205	9.276846E-11	14.439025	24
AGTATCA	540	0.0	14.046295	1
>>END_MODULE