FastQCFastQC Report
Wed 25 May 2016
SRR2088270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176783
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT219411.0079553175488782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199790.9178223093436508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186580.857136425633607No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG127910.5876102487018687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77890.35782161106550353No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69440.31900285880586166No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68800.3160627402915219No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT65850.3025106315144872No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT64810.29773293892868513No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA49220.22611348949344054No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT48950.22487312699520345No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG46410.21320453164141764No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43560.20011181638224848No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA38550.17709620113718272No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37860.17392638586391018No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37210.1709403279977839No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28070.12895176046486947No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26600.12219867575224541No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25980.11935043594147879No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.11553746974319443No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC23790.10928971790022249No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG23520.10804935540198542No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23190.10653335679302897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.028.9252031
GTATTGG7950.020.7106911
ATTGGAC8350.018.6107793
TGTACCG609.240432E-418.55
GTATCAA85300.017.068582
ACGTTTA1107.815488E-716.81818226
TTGGACC13350.016.4906374
TATTGGA10050.016.3830832
GCGTTAT803.384304E-416.18751
CCGTCGA803.384304E-416.18759
ACCGTCC803.384304E-416.18758
GGACCCT13650.015.4505496
TGGACCC14500.015.3103455
CGATCGA750.004105972614.81
ACGACGC750.004105972614.827
TACGTTA1001.0940768E-414.819
CGTCTGT4550.014.63736334
ACGGTGT1051.6567044E-414.09523829
TGCGTTA1601.7854654E-713.87537
AACAACG2151.9826984E-1013.7674422
Produced by FastQC (version 0.11.5)