##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2176783
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.096069750636605	31.0	30.0	33.0	27.0	34.0
2	30.87534586589476	31.0	31.0	34.0	27.0	34.0
3	30.833943025097128	31.0	31.0	34.0	27.0	34.0
4	35.00622386338004	35.0	35.0	37.0	32.0	37.0
5	34.908886646027646	35.0	35.0	37.0	32.0	37.0
6	35.01191437088585	36.0	35.0	37.0	32.0	37.0
7	34.639529985304	35.0	35.0	37.0	30.0	37.0
8	34.85229487734882	35.0	35.0	37.0	32.0	37.0
9	36.29644066496293	38.0	35.0	39.0	32.0	39.0
10	36.213047878451825	38.0	35.0	39.0	32.0	39.0
11	36.30173747222392	38.0	35.0	39.0	32.0	39.0
12	36.03905809628245	38.0	35.0	39.0	30.0	39.0
13	36.20942785753105	38.0	35.0	39.0	32.0	39.0
14	37.14142061932678	39.0	36.0	40.0	31.0	41.0
15	37.179392249939475	39.0	36.0	40.0	32.0	41.0
16	37.10712689321811	39.0	36.0	40.0	31.0	41.0
17	37.17698364972531	39.0	36.0	40.0	31.0	41.0
18	37.17371828060032	39.0	36.0	40.0	31.0	41.0
19	37.25523031004928	39.0	36.0	40.0	31.0	41.0
20	37.20917335352215	39.0	36.0	40.0	31.0	41.0
21	37.13428394102674	39.0	36.0	40.0	31.0	41.0
22	37.05049653548379	39.0	36.0	40.0	31.0	41.0
23	36.93254908734587	39.0	36.0	40.0	31.0	41.0
24	36.866920588777106	39.0	35.0	40.0	31.0	41.0
25	36.7809845997511	39.0	35.0	40.0	30.0	41.0
26	36.5273428724866	38.0	35.0	40.0	30.0	41.0
27	36.39042614720898	38.0	35.0	40.0	30.0	41.0
28	36.251808747128216	38.0	35.0	40.0	30.0	41.0
29	36.08643672796048	38.0	35.0	40.0	29.0	41.0
30	35.94057698907057	38.0	34.0	40.0	29.0	41.0
31	35.71369769058285	38.0	34.0	40.0	28.0	41.0
32	35.475354686250306	38.0	34.0	40.0	27.0	41.0
33	35.64156693616222	38.0	34.0	40.0	28.0	41.0
34	35.64787027462085	38.0	34.0	40.0	28.0	41.0
35	35.57116763591042	38.0	34.0	40.0	27.0	41.0
36	35.49741246601062	38.0	34.0	40.0	27.0	41.0
37	35.364019748408545	38.0	34.0	40.0	27.0	41.0
38	35.259381389876715	38.0	34.0	40.0	26.0	41.0
39	35.26130257356843	38.0	34.0	40.0	26.0	41.0
40	35.150990705090955	38.0	34.0	40.0	25.0	41.0
41	35.011075058928704	38.0	33.0	40.0	25.0	41.0
42	34.85265871701497	38.0	33.0	40.0	24.0	41.0
43	34.528941102535256	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	7.0
16	19.0
17	55.0
18	154.0
19	367.0
20	780.0
21	1582.0
22	2894.0
23	5083.0
24	8456.0
25	13344.0
26	20467.0
27	29972.0
28	41660.0
29	56217.0
30	72643.0
31	90316.0
32	111138.0
33	134688.0
34	162897.0
35	195135.0
36	237486.0
37	297658.0
38	357354.0
39	336405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.95694563950563	24.38791556163384	12.966060466293609	22.68907833256691
2	17.969315269367687	22.74797258155728	36.12215824912267	23.16055389995236
3	19.533504258348213	26.847738153045114	31.596213311110937	22.022544277495737
4	13.100800585083586	15.964430078698705	36.08209913436479	34.852670201852916
5	11.556641153481996	40.060217302321824	35.082964172358935	13.300177371837249
6	31.721489923432884	36.687855427022356	15.619884940299517	15.97076970924525
7	25.02270552462051	34.398743466849936	22.105694504229405	18.472856504300154
8	27.2862752051996	34.1774076699423	20.229852952728866	18.30646417212924
9	25.320714099659913	13.68684889582471	22.972524133089976	38.019912871425404
10	16.031547471658865	27.404339339291056	34.39520613676237	22.16890705228771
11	31.919258832874014	21.93732677993167	24.82672824989905	21.316686137295264
12	20.042787912254	26.911134458510567	32.12460773535993	20.921469893875503
13	30.712202364682195	20.923491225354113	28.270387999171255	20.09391841079244
14	20.947241870227764	22.145891437042646	28.047536203654662	28.859330489074935
15	23.182145395292043	28.87715495756812	26.26297614415401	21.67772350298583
16	22.07601768297529	28.48855398080562	26.685434423183203	22.749993913035887
17	19.814193697764086	27.161595804450876	27.80355230631625	25.220658191468786
18	20.897581430946495	26.63683058899302	31.337574760552613	21.12801321950787
19	20.438509488543417	24.9888941617056	32.4811889839272	22.091407365823788
20	24.16566097769047	24.4095989356771	31.35080529386714	20.07393479276529
21	23.599825981735435	25.111644109679283	30.26369647319002	21.02483343539526
22	22.235702869785367	24.638698483036663	31.225758378304132	21.899840268873838
23	21.491577249546694	26.378605492600776	31.972915995760715	20.15690126209181
24	20.367625068736757	25.515818526697426	30.63814812960226	23.47840827496356
25	21.431442638058087	26.397991899054706	31.401522338239502	20.769043124647702
26	22.60928167851366	25.576688167814616	30.302515225449667	21.51151492822206
27	21.40672726679692	26.872177888195566	30.04387667489134	21.677218170116173
28	21.262983035056777	26.17596701187027	30.26043477921318	22.30061517385977
29	20.428632527909304	26.128787297585475	31.174995394579984	22.26758477992524
30	19.940572854528906	26.473332435984666	32.45054743628557	21.135547273200867
31	21.23468439435626	25.93983874368736	31.61380808284519	21.211668779111193
32	21.94876567852652	26.106690469376137	29.98943854302427	21.955105309073065
33	22.701206321438562	26.082250734225692	29.599505325059962	21.617037619275784
34	21.334005272918798	28.165232822931824	29.729789326726642	20.77097257742274
35	20.22231889903587	29.196295634429337	29.779403826656125	20.801981639878665
36	21.161319249553127	26.144682313303626	31.476357542299805	21.217640894843445
37	21.17726020462306	26.366615321784487	30.79186120068009	21.664263272912365
38	21.313608200725568	26.053768336118022	31.26287737454767	21.36974608860874
39	22.060811757533937	25.542095835919337	31.325998043902402	21.071094362644324
40	20.720117714995016	26.11555676427095	30.55688141629184	22.607444104442198
41	21.607849748918472	25.79692142027937	30.37404279618134	22.221186034620814
42	19.448194882080575	27.272217763552913	30.825764442298563	22.45382291206795
43	20.052848630295255	26.949907271418418	30.43367207480029	22.563572023486035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	182.5
2	259.0
3	758.5
4	1258.0
5	1258.0
6	2155.5
7	3053.0
8	3333.5
9	3614.0
10	5567.5
11	7521.0
12	7521.0
13	13922.0
14	20323.0
15	32617.0
16	44911.0
17	44384.5
18	43858.0
19	43858.0
20	47665.5
21	51473.0
22	43455.5
23	35438.0
24	39503.0
25	43568.0
26	43568.0
27	48710.5
28	53853.0
29	58120.0
30	62387.0
31	67761.0
32	73135.0
33	73135.0
34	79851.5
35	86568.0
36	94193.0
37	101818.0
38	105569.5
39	109321.0
40	109321.0
41	115125.0
42	120929.0
43	133597.5
44	146266.0
45	175285.5
46	204305.0
47	204305.0
48	206554.5
49	208804.0
50	192553.0
51	176302.0
52	166185.0
53	156068.0
54	156068.0
55	137120.5
56	118173.0
57	99678.5
58	81184.0
59	72213.5
60	63243.0
61	63243.0
62	56064.0
63	48885.0
64	43046.5
65	37208.0
66	31762.5
67	26317.0
68	26317.0
69	22219.0
70	18121.0
71	15028.0
72	11935.0
73	9605.0
74	7275.0
75	7275.0
76	5747.0
77	4219.0
78	3359.5
79	2500.0
80	1885.5
81	1271.0
82	1271.0
83	947.5
84	624.0
85	480.0
86	336.0
87	278.0
88	220.0
89	220.0
90	162.5
91	105.0
92	64.0
93	23.0
94	13.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2176783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.76743387520364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52790165291783	38.08597285119676
2	13.207160345697044	13.145729583673788
3	4.632137591356728	6.915888038360732
4	1.9667094050923304	3.9151232107869456
5	0.9578572398209128	2.3835048422336182
6	0.5842259508934958	1.7445255827562016
7	0.37184890437534174	1.295417603204672
8	0.2496913211902548	0.9941197053238591
9	0.19971616359429012	0.8945424868939369
>10	1.1297485717986226	10.834891502419158
>50	0.10745273753985238	3.6616842978183537
>100	0.055841466124167204	5.136146374666599
>500	0.004107505599542274	1.4423225712349976
>1k	0.004760972399469452	4.530319167861718
>5k	4.6676199994798547E-4	1.61115491859747
>10k+	3.7340959995838836E-4	3.408657262971187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21941	1.0079553175488782	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19979	0.9178223093436508	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18658	0.857136425633607	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12791	0.5876102487018687	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7789	0.35782161106550353	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6944	0.31900285880586166	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6880	0.3160627402915219	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6585	0.3025106315144872	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6481	0.29773293892868513	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4922	0.22611348949344054	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4895	0.22487312699520345	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4641	0.21320453164141764	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4356	0.20011181638224848	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3855	0.17709620113718272	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3786	0.17392638586391018	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3721	0.1709403279977839	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2807	0.12895176046486947	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2660	0.12219867575224541	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2598	0.11935043594147879	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2515	0.11553746974319443	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2379	0.10928971790022249	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2352	0.10804935540198542	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2319	0.10653335679302897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.593935178655842E-5	0.0
2	0.0	0.0	0.0	4.593935178655842E-5	0.0
3	0.0	0.0	0.0	4.593935178655842E-5	0.0
4	0.0	0.0	0.0	9.187870357311684E-5	0.0
5	4.593935178655842E-5	0.0	0.0	9.187870357311684E-5	0.0
6	4.593935178655842E-5	0.0	0.0	9.187870357311684E-5	0.0
7	9.187870357311684E-5	0.0	0.0	9.187870357311684E-5	0.0
8	9.187870357311684E-5	0.0	0.0	9.187870357311684E-5	0.0
9	9.187870357311684E-5	0.0	0.0	9.187870357311684E-5	0.0
10	9.187870357311684E-5	0.0	0.0	1.837574071462337E-4	0.0
11	9.187870357311684E-5	0.0	0.0	4.5939351786558423E-4	0.0
12	9.187870357311684E-5	0.0	0.0	7.350296285849348E-4	0.0
13	9.187870357311684E-5	0.0	0.0	7.809689803714931E-4	0.0
14	9.187870357311684E-5	0.0	0.0	8.269083321580516E-4	0.0
15	9.187870357311684E-5	0.0	0.0	0.001515998608956428	0.0
16	9.187870357311684E-5	0.0	0.0	0.002388846292901038	0.0
17	9.187870357311684E-5	0.0	0.0	0.0037670268464977904	0.0
18	9.187870357311684E-5	0.0	0.0	0.0042723597161499335	0.0
19	9.187870357311684E-5	0.0	0.0	0.004915510641161751	0.0
20	9.187870357311684E-5	0.0	0.0	0.005650540269746686	0.0
21	9.187870357311684E-5	0.0	0.0	0.0074881143412090225	0.0
22	9.187870357311684E-5	0.0	0.0	0.013827744887754085	0.0
23	9.187870357311684E-5	0.0	0.0	0.02590979440761895	0.0
24	9.187870357311684E-5	0.0	0.0	0.042310142995420304	0.0
25	9.187870357311684E-5	0.0	0.0	0.04929292446697719	0.0
26	9.187870357311684E-5	0.0	0.0	0.05609194853138783	0.0
27	9.187870357311684E-5	0.0	0.0	0.1061658419787365	0.0
28	9.187870357311684E-5	0.0	0.0	0.15862858171898622	0.0
29	9.187870357311684E-5	0.0	0.0	0.22445967282912443	0.0
30	9.187870357311684E-5	0.0	0.0	0.34381010877060325	0.0
31	9.187870357311684E-5	0.0	0.0	0.5038168710431862	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4880	0.0	28.925203	1
GTATTGG	795	0.0	20.710691	1
ATTGGAC	835	0.0	18.610779	3
TGTACCG	60	9.240432E-4	18.5	5
GTATCAA	8530	0.0	17.06858	2
ACGTTTA	110	7.815488E-7	16.818182	26
TTGGACC	1335	0.0	16.490637	4
TATTGGA	1005	0.0	16.383083	2
GCGTTAT	80	3.384304E-4	16.1875	1
CCGTCGA	80	3.384304E-4	16.1875	9
ACCGTCC	80	3.384304E-4	16.1875	8
GGACCCT	1365	0.0	15.450549	6
TGGACCC	1450	0.0	15.310345	5
CGATCGA	75	0.0041059726	14.8	1
ACGACGC	75	0.0041059726	14.8	27
TACGTTA	100	1.0940768E-4	14.8	19
CGTCTGT	455	0.0	14.637363	34
ACGGTGT	105	1.6567044E-4	14.095238	29
TGCGTTA	160	1.7854654E-7	13.875	37
AACAACG	215	1.9826984E-10	13.767442	2
>>END_MODULE