FastQCFastQC Report
Wed 25 May 2016
SRR2088269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2420443
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310511.2828643351650917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291221.2031681803702876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257771.0649703380744764No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG155170.6410809921985355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107280.44322464937203643No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA94220.38926758448763304No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA93810.38757367969417167No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT91010.37600554939736236No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT88520.36571817638341414No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA69300.2863112248460302No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT64480.2663975148350943No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG61440.25383783051284414No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT59580.24615328681567797No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51890.21438224325051242No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48650.20099626390706166No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48160.19897184110512No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT42090.17389378721167986No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37860.1564176475132858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37370.15439322471134417No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35730.14761760553749873No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC33790.13960254383185228No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG31050.12828230204140315No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30560.12625787923946152No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30450.12580341697780117No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28960.11964751906985623No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26570.10977329356650829No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26250.10845122153258721No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC25110.10374133991174342No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.10097325159072121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75000.029.7973351
ATTGGAC9250.022.03
GTATTGG8850.021.9491521
TTGGACC15650.019.2683724
TATTGGA11850.018.5780582
GTATCAA125950.018.375152
GGACCCT17050.017.9032276
TGGACCC17000.017.7382355
ACGTGCG852.72513E-517.4117649
CGCGATA752.0684575E-417.26666614
TCCGTTA752.0684575E-417.26666614
TACGTGC752.0684575E-417.2666668
CGTCTGT7250.017.09655234
GTAATAC1851.8189894E-1217.03
AGTATCA4600.016.489131
TAGGTCG1352.2226232E-816.44444521
CATTGCG1606.311893E-1016.187529
TGCGACG1151.2435066E-616.08695622
GACCCTC19100.016.0785337
CGGGTCA700.002593604415.85714334
Produced by FastQC (version 0.11.5)