FastQCFastQC Report
Wed 25 May 2016
SRR2088268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438166
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197771.3751541894329307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT175231.218426801912992No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT146541.0189366178869477No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG97600.6786421039017749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64050.4453588806855398No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT62890.43729305240146127No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA62390.43381640227901375No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59570.4142080955884091No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT57860.40231795216963834No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA45700.3177658211917122No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG41230.2866845690970305No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT39680.2759069537174429No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT39160.2722912375900974No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34090.23703800534847858No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA32370.22507832892725874No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA28860.20067224506767647No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT25240.17550129818115573No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA24840.17271997808319764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23830.16569714483585346No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC22310.15512812846361268No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG22040.15325073739749095No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20650.1435856500570866No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19570.13607608579259972No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18600.12933138455505136No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18140.12613286644239954No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC17790.12369921135668623No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA16960.11792797215342318No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15870.11034887488648737No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA15870.11034887488648737No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA14540.10110098556077671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.031.053571
ATTGGAC4950.023.5454543
ATAACAC1001.2903911E-820.3499983
GTATTGG5550.020.01
GTATCAA72000.018.731252
TATTGGA6350.018.645672
CGTCTGT3600.018.534
GGACCGT801.617131E-518.56
ACCGTAG500.007035721618.4999988
TTGGACC8650.018.3930644
GTGGTAT5050.017.584161
TCACGTT953.6076617E-617.52631624
GGACCCT8400.017.1785726
TGGACCC9150.016.5792335
AGGTCGT803.3833052E-416.187522
TAGGTCG700.002592918215.85714221
ACTTCGT700.002592918215.85714225
AGGTAAC855.365315E-415.2352941
TTATACT3300.015.1363644
GGTCGGC750.00410507214.830
Produced by FastQC (version 0.11.5)