FastQCFastQC Report
Wed 25 May 2016
SRR2088267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955770
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT373711.26434059483654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT348621.1794557763290108No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313731.061415468727269No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG213270.7215378733798637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128630.4351827104274013No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA128120.4334572717092331No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA123790.4188079586706679No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT115000.3890695148810631No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT110700.3745216982376843No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA89240.301917943547705No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT86190.29159914337042464No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT80150.2711645358062366No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG79970.27055555743511844No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA68860.23296805908443485No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA68390.2313779488931818No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA63660.21537535058546503No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT53420.18073124769518603No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA49880.16875467306319503No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.1535302137852404No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC45350.15342871739005404No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44200.14953802224124338No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40280.13627582660355847No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG39480.1335692560652554No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38960.13180998521535844No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35720.12084837453523109No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC34690.11736366496716592No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA31980.10819515726866434No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC31120.10528559393998857No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.10305267324588856No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG29660.1003461027075855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81450.030.299571
GTATTGG11250.021.5422211
ATTGGAC12250.020.3877543
TCTAACG759.273857E-619.7333342
CGTGTCT2550.018.13725535
TATTGGA14650.018.058022
ACGTTTA1750.017.9714326
GTATCAA142500.017.9547392
TATACTG4150.017.8313265
TTGGACC19100.017.5314144
TGGACCC20700.016.4444435
TATCGGC1251.6601916E-716.2799992
TCACGTT1851.8189894E-1116.00000224
TCGCTTA700.002593786215.85714329
GGACCCT20900.015.75598056
CGCACTA957.065681E-515.57894629
CGACGAG1557.221388E-915.51612924
GCCGTCA2109.094947E-1214.9761913
TAGGTCG1904.5656634E-1014.60526221
ATCGATA1152.2120572E-514.47826123
Produced by FastQC (version 0.11.5)