##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2955770
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.007358150329694	31.0	30.0	33.0	27.0	34.0
2	30.825615998538453	31.0	31.0	34.0	27.0	34.0
3	30.795245232206835	31.0	31.0	34.0	27.0	34.0
4	34.98399435680043	35.0	35.0	37.0	32.0	37.0
5	34.90107789171688	35.0	35.0	37.0	32.0	37.0
6	35.00982248280482	36.0	35.0	37.0	32.0	37.0
7	34.62490552377215	35.0	35.0	37.0	30.0	37.0
8	34.84879100877267	35.0	35.0	37.0	32.0	37.0
9	36.257718293371944	38.0	35.0	39.0	32.0	39.0
10	36.20001623942323	38.0	35.0	39.0	32.0	39.0
11	36.2942289149697	38.0	35.0	39.0	32.0	39.0
12	36.02601826258471	38.0	35.0	39.0	30.0	39.0
13	36.19376203155185	38.0	35.0	39.0	32.0	39.0
14	37.09457163446412	39.0	36.0	40.0	31.0	41.0
15	37.13658505228756	39.0	36.0	40.0	31.0	41.0
16	37.06397250124333	39.0	36.0	40.0	31.0	41.0
17	37.160590303034404	39.0	36.0	40.0	31.0	41.0
18	37.16409362027492	39.0	36.0	40.0	31.0	41.0
19	37.254279595503036	39.0	36.0	40.0	31.0	41.0
20	37.216570978120764	39.0	36.0	40.0	31.0	41.0
21	37.14416175818822	39.0	36.0	40.0	31.0	41.0
22	37.069420827736934	39.0	36.0	40.0	31.0	41.0
23	36.94241635851233	39.0	35.0	40.0	31.0	41.0
24	36.86621996975408	39.0	35.0	40.0	31.0	41.0
25	36.78741444699689	38.0	35.0	40.0	30.0	41.0
26	36.521666435480434	38.0	35.0	40.0	30.0	41.0
27	36.38768916390653	38.0	35.0	40.0	30.0	41.0
28	36.235586666080245	38.0	35.0	40.0	30.0	41.0
29	36.071884145248106	38.0	35.0	40.0	30.0	41.0
30	35.90680431833329	38.0	34.0	40.0	29.0	41.0
31	35.643750697787716	38.0	34.0	40.0	28.0	41.0
32	35.415821934724285	38.0	34.0	40.0	27.0	41.0
33	35.53381792223346	38.0	34.0	40.0	28.0	41.0
34	35.52828535373185	38.0	34.0	40.0	28.0	41.0
35	35.432612821701284	38.0	34.0	40.0	27.0	41.0
36	35.35225677234697	38.0	34.0	40.0	27.0	41.0
37	35.195724633513436	38.0	34.0	40.0	26.0	41.0
38	35.070551159257995	38.0	33.0	40.0	25.0	41.0
39	35.03658843550073	38.0	33.0	40.0	25.0	41.0
40	34.87510158097552	38.0	33.0	40.0	24.0	41.0
41	34.71474539629268	38.0	33.0	40.0	24.0	41.0
42	34.50809467583743	38.0	33.0	40.0	23.0	41.0
43	34.17205567415597	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	6.0
15	7.0
16	32.0
17	85.0
18	214.0
19	544.0
20	1101.0
21	2273.0
22	4229.0
23	7165.0
24	12065.0
25	19007.0
26	28727.0
27	42078.0
28	58919.0
29	79135.0
30	101847.0
31	125483.0
32	152902.0
33	184969.0
34	223190.0
35	266220.0
36	323951.0
37	407336.0
38	471305.0
39	442977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.52912439059873	24.99873129506017	13.452332218000723	22.019812096340377
2	17.828450792856007	22.908142379143168	36.35465547048654	22.908751357514284
3	19.616208297668628	26.791563619632107	32.21454984657128	21.37767823612798
4	12.79497389851037	15.855428534696541	35.652165087269985	35.6974324795231
5	11.031440200015563	40.63188272429857	34.99500976057001	13.341667315115856
6	31.560371747463435	36.18576546889643	15.373388321824768	16.880474461815364
7	24.41952519986332	35.2620129441736	21.559052294326015	18.75940956163707
8	27.772052629264117	33.609076484300196	20.08762522117756	18.531245665258123
9	25.007561481441382	13.649099896135356	23.7323607723199	37.61097785010335
10	16.207012047622108	27.73656272308062	34.0107653843161	22.045659844981174
11	31.314242989136503	21.760251981717115	25.27513981128437	21.65036521786201
12	20.19707216732019	27.300026727384065	31.85386549021067	20.649035615085072
13	31.22472316858213	20.81741813469925	28.842974927007177	19.114883769711447
14	20.825368685655516	22.517990236046785	28.374433734695188	28.28220734360251
15	22.913420191692857	28.959391292285936	26.91474641125663	21.212442104764577
16	21.80480889920393	28.402751228952184	27.125994241771178	22.666445630072705
17	19.276364534452952	26.982951988821863	27.964760451591292	25.77592302513389
18	20.507380479536636	26.351712075026136	32.50060052033818	20.640306925099043
19	19.695307821650534	24.587298741106377	33.872459629808816	21.844933807434273
20	24.430825131860733	23.644904711800983	32.450934950960324	19.473335205377957
21	23.677349726128895	24.609357290993547	31.134695866051825	20.578597116825733
22	21.89943737164935	24.208751019192967	32.12448871190925	21.767322897248434
23	21.070110326581567	26.34609594115916	33.12436353302185	19.459430199237424
24	19.553551189706912	25.253284254187573	31.582802450799623	23.610362105305892
25	20.862787023347554	26.292776501554588	32.717227659797615	20.127208815300243
26	22.28718743339299	25.286981057389447	31.302469407294886	21.12336210192268
27	20.792619182142047	26.822283195241848	30.90622748048732	21.478870142128788
28	20.652757149575237	26.0948923630729	31.14542065180985	22.106929835542008
29	19.730459406516747	26.07709666178356	32.04041586456321	22.15202806713648
30	19.057673634958064	26.387607966790377	33.96285908578814	20.59185931246342
31	20.578258795508447	25.72311106750525	32.8758665254739	20.8227636115124
32	21.670968986084844	26.020698498191674	30.547234730713146	21.761097785010335
33	22.66052500702017	25.885742124725535	30.166014270393166	21.287718597861133
34	20.819245069812602	28.81966458824604	30.256109237186923	20.10498110475443
35	19.40255838580133	30.040869215128374	30.348606285333435	20.207966113736862
36	20.67613515259983	26.02590864647791	32.60186009060245	20.696096110319814
37	20.601467637874396	26.66043027705133	31.520754321209026	21.217347763865256
38	20.892017985161225	26.157414142507708	32.1551067911238	20.795461081207268
39	21.95945557333622	25.4562770445603	32.17094022877288	20.413327153330606
40	20.02100975380358	26.48311607466075	31.117779800187428	22.378094371348244
41	21.311401090071282	25.81499913728064	31.010667271134086	21.86293250151399
42	18.620190339573107	27.704151540884443	31.46865283834669	22.207005281195762
43	19.380432171650703	27.481231624923453	30.99442784790427	22.143908355521575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	330.5
2	433.0
3	1172.5
4	1912.0
5	1912.0
6	3346.5
7	4781.0
8	5299.5
9	5818.0
10	8997.0
11	12176.0
12	12176.0
13	22760.0
14	33344.0
15	54598.5
16	75853.0
17	73647.0
18	71441.0
19	71441.0
20	76692.5
21	81944.0
22	66316.5
23	50689.0
24	55874.5
25	61060.0
26	61060.0
27	66839.5
28	72619.0
29	76467.5
30	80316.0
31	86204.0
32	92092.0
33	92092.0
34	99908.5
35	107725.0
36	117323.0
37	126921.0
38	132954.0
39	138987.0
40	138987.0
41	146816.0
42	154645.0
43	175561.0
44	196477.0
45	245951.0
46	295425.0
47	295425.0
48	300803.5
49	306182.0
50	278660.0
51	251138.0
52	235244.5
53	219351.0
54	219351.0
55	188586.5
56	157822.0
57	128878.5
58	99935.0
59	87146.0
60	74357.0
61	74357.0
62	65735.5
63	57114.0
64	49553.0
65	41992.0
66	35508.5
67	29025.0
68	29025.0
69	24485.5
70	19946.0
71	17059.0
72	14172.0
73	11435.0
74	8698.0
75	8698.0
76	6917.5
77	5137.0
78	3982.5
79	2828.0
80	2243.5
81	1659.0
82	1659.0
83	1222.0
84	785.0
85	575.5
86	366.0
87	290.5
88	215.0
89	215.0
90	165.0
91	115.0
92	68.0
93	21.0
94	17.0
95	13.0
96	13.0
97	12.5
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2955770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.72594420647387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.74009782324701	27.782030256700413
2	14.155425644254532	10.96364447436581
3	5.563569446472486	6.463634399188085
4	2.7428840891967843	4.2488310481223825
5	1.5116135449312667	2.9269330901379207
6	0.8899536145021609	2.0678576412936263
7	0.5869439993453405	1.5910972399680603
8	0.4421227276458008	1.3697296066580262
9	0.30819375853942776	1.0741584868183223
>10	1.7565983404374979	13.004803699285445
>50	0.1808607622108388	4.8717598475777315
>100	0.10588033595626097	7.586286335913803
>500	0.007086441680731337	1.8637855392890579
>1k	0.007263602722747848	5.796663922081743
>5k	7.086441680729607E-4	2.0235033017662216
>10k+	7.972246890820807E-4	6.365281110833301
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37371	1.26434059483654	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34862	1.1794557763290108	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31373	1.061415468727269	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	21327	0.7215378733798637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12863	0.4351827104274013	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12812	0.4334572717092331	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12379	0.4188079586706679	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11500	0.3890695148810631	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11070	0.3745216982376843	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8924	0.301917943547705	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8619	0.29159914337042464	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8015	0.2711645358062366	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7997	0.27055555743511844	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6886	0.23296805908443485	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6839	0.2313779488931818	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6366	0.21537535058546503	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5342	0.18073124769518603	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4988	0.16875467306319503	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4538	0.1535302137852404	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4535	0.15342871739005404	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4420	0.14953802224124338	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4028	0.13627582660355847	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3948	0.1335692560652554	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3896	0.13180998521535844	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3572	0.12084837453523109	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3469	0.11736366496716592	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	3198	0.10819515726866434	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3112	0.10528559393998857	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3046	0.10305267324588856	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2966	0.1003461027075855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.38321317287881E-5	0.0
9	0.0	0.0	0.0	6.76642634575762E-5	0.0
10	0.0	0.0	0.0	1.014963951863643E-4	0.0
11	0.0	0.0	0.0	2.706570538303048E-4	0.0
12	0.0	0.0	0.0	5.751462393893976E-4	0.0
13	0.0	0.0	0.0	6.089783711181858E-4	0.0
14	0.0	0.0	0.0	7.104747663045501E-4	0.0
15	0.0	0.0	0.0	0.001387117400880312	0.0
16	0.0	0.0	0.0	0.0020637600354560743	0.0
17	0.0	0.0	0.0	0.0029433954604045644	0.0
18	0.0	0.0	0.0	0.003450877436336386	0.0
19	0.0	0.0	0.0	0.004195184334369724	0.0
20	0.0	0.0	0.0	0.005108651891047003	0.0
21	0.0	0.0	0.0	0.006732594214028832	0.0
22	0.0	0.0	0.0	0.011705917578160682	0.0
23	0.0	0.0	0.0	0.020772928881475892	0.0
24	0.0	0.0	0.0	0.03352764254322901	0.0
25	0.0	0.0	0.0	0.039245272805394193	0.0
26	0.0	0.0	0.0	0.04658684539054121	0.0
27	0.0	0.0	0.0	0.10024460631239913	0.0
28	0.0	0.0	0.0	0.15559397382069648	0.0
29	0.0	0.0	0.0	0.22332590154173024	0.0
30	3.38321317287881E-5	0.0	0.0	0.3533089516437341	0.0
31	3.38321317287881E-5	0.0	0.0	0.5271046123345186	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8145	0.0	30.29957	1
GTATTGG	1125	0.0	21.542221	1
ATTGGAC	1225	0.0	20.387754	3
TCTAACG	75	9.273857E-6	19.733334	2
CGTGTCT	255	0.0	18.137255	35
TATTGGA	1465	0.0	18.05802	2
ACGTTTA	175	0.0	17.97143	26
GTATCAA	14250	0.0	17.954739	2
TATACTG	415	0.0	17.831326	5
TTGGACC	1910	0.0	17.531414	4
TGGACCC	2070	0.0	16.444443	5
TATCGGC	125	1.6601916E-7	16.279999	2
TCACGTT	185	1.8189894E-11	16.000002	24
TCGCTTA	70	0.0025937862	15.857143	29
GGACCCT	2090	0.0	15.7559805	6
CGCACTA	95	7.065681E-5	15.578946	29
CGACGAG	155	7.221388E-9	15.516129	24
GCCGTCA	210	9.094947E-12	14.97619	13
TAGGTCG	190	4.5656634E-10	14.605262	21
ATCGATA	115	2.2120572E-5	14.478261	23
>>END_MODULE