FastQCFastQC Report
Wed 25 May 2016
SRR2088266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305751
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371921.6130102513237554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340931.478607186985932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT293091.2711259802120871No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG159380.6912281508280816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125650.5449417565036294No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT103130.44727292756242976No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA98640.42779987951864706No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT94640.4104519525308674No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87890.38117732573898916No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA75990.32956724295034456No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT69530.30155034086508037No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67000.29057777704530974No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT66960.290404297775432No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57970.2514148318703971No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56470.24490935924997972No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT48890.21203503760813722No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA47890.20769805586119233No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.19568461642215487No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA41800.18128583702229772No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC40510.17569113056873878No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36640.15890701120806192No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.14767422848347458No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33620.14580932633228827No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG32370.14038809914860712No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT32300.14008451042632097No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31630.13717873265586789No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27650.11991754530302709No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC27620.11978743585061874No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA27040.11727198643739069No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG26150.11341207268260971No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC25200.10929194002301203No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC24730.10725355860194792No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC23880.10356712411704473No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA23330.10118178415622502No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA23080.1000975387194888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84000.029.1595231
GTATTGG7550.023.523181
ATTGGAC8200.021.8841463
CGCGATA603.7283808E-521.58333214
TAGGTCG957.1540853E-921.42105321
TTGGACC15400.020.3019474
TGCGCGA656.905982E-519.92307712
TATTGGA9950.019.7085422
GGACCCT17200.018.1773246
ACCGACC2350.018.1063848
TGGACCC17500.018.0771435
GTATCAA146050.017.3663122
CGAATTA1603.45608E-1117.3437515
CTGTGCG1401.87174E-917.1785729
GTAATAC1554.0199666E-1016.7096793
CGACCGC2000.016.6510
CGTCTGT7950.016.52201334
CCGACAC904.4490804E-516.44444533
GACCCTC18950.016.4010567
CCGTCGA803.3844108E-416.18759
Produced by FastQC (version 0.11.5)