##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2305751
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.920344391046562	31.0	30.0	33.0	27.0	34.0
2	30.7631049493202	31.0	31.0	34.0	27.0	34.0
3	30.77277858710676	31.0	31.0	34.0	27.0	34.0
4	34.95785017549596	35.0	35.0	37.0	32.0	37.0
5	34.87219912297555	35.0	35.0	37.0	32.0	37.0
6	34.98333037695744	35.0	35.0	37.0	32.0	37.0
7	34.58877172773643	35.0	35.0	37.0	30.0	37.0
8	34.82802913237379	35.0	35.0	37.0	32.0	37.0
9	36.215912299289904	38.0	35.0	39.0	32.0	39.0
10	36.159996027324716	38.0	35.0	39.0	32.0	39.0
11	36.25492518489637	38.0	35.0	39.0	32.0	39.0
12	35.99637645175043	38.0	35.0	39.0	30.0	39.0
13	36.17554193839664	38.0	35.0	39.0	32.0	39.0
14	37.0401708597329	39.0	36.0	40.0	31.0	41.0
15	37.089654954069196	39.0	36.0	40.0	31.0	41.0
16	36.99027475213065	38.0	36.0	40.0	31.0	41.0
17	37.122945192260566	39.0	36.0	40.0	31.0	41.0
18	37.155593557153395	39.0	36.0	40.0	31.0	41.0
19	37.25051707664878	39.0	36.0	40.0	31.0	41.0
20	37.20976679615448	39.0	36.0	40.0	31.0	41.0
21	37.150555719156145	39.0	36.0	40.0	31.0	41.0
22	37.074098200542906	39.0	36.0	40.0	31.0	41.0
23	36.95320917132856	39.0	35.0	40.0	31.0	41.0
24	36.8769286015706	39.0	35.0	40.0	31.0	41.0
25	36.785659422895186	38.0	35.0	40.0	30.0	41.0
26	36.51137525257497	38.0	35.0	40.0	30.0	41.0
27	36.35319425211135	38.0	35.0	40.0	30.0	41.0
28	36.17832844916906	38.0	35.0	40.0	30.0	41.0
29	35.999665185009135	38.0	34.0	40.0	30.0	41.0
30	35.81984958479905	38.0	34.0	40.0	29.0	41.0
31	35.53097710897664	38.0	34.0	40.0	28.0	41.0
32	35.30100865184489	37.0	34.0	40.0	27.0	41.0
33	35.35130506286238	38.0	34.0	40.0	27.0	41.0
34	35.317944565566705	38.0	34.0	40.0	27.0	41.0
35	35.19691371704924	38.0	34.0	40.0	27.0	41.0
36	35.09129606796224	38.0	34.0	40.0	26.0	41.0
37	34.909499768188326	38.0	33.0	40.0	25.0	41.0
38	34.7470563820638	38.0	33.0	40.0	25.0	41.0
39	34.65511150163222	38.0	33.0	40.0	24.0	41.0
40	34.44101119331619	38.0	33.0	40.0	23.0	41.0
41	34.24723094557912	38.0	33.0	40.0	22.0	41.0
42	34.00937438604602	38.0	33.0	40.0	21.0	41.0
43	33.64360548905758	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	10.0
16	30.0
17	75.0
18	222.0
19	424.0
20	1081.0
21	2016.0
22	3678.0
23	6459.0
24	10382.0
25	16188.0
26	24351.0
27	35071.0
28	49056.0
29	65110.0
30	82421.0
31	100373.0
32	121828.0
33	148067.0
34	176194.0
35	208624.0
36	252852.0
37	316167.0
38	352519.0
39	332550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14095667745563	25.651772459385253	14.130753927895945	21.076516935263175
2	17.951721586589358	23.302386077247718	36.03368273503948	22.71220960112345
3	20.060405481771447	26.395261240264016	32.816292826068384	20.728040451896153
4	13.023218899178618	15.90358195659462	34.70500500704542	36.368194137181334
5	10.714513405827429	41.32497394558216	34.3877547922564	13.572757856334011
6	31.43843372506398	35.57222787716453	15.092089301923755	17.89724909584773
7	23.45235890605707	36.63235969538775	21.073090719683087	18.84219067887209
8	28.19441474816665	33.40742343817698	20.003721130338878	18.394440683317494
9	24.876623711753783	13.670383315457741	24.630846956154414	36.82214601663406
10	16.68965989822839	28.484775676124613	33.12564973407797	21.699914691569038
11	30.60321778023733	21.78084277096703	25.397581959196806	22.218357489598834
12	20.054420446960663	28.234054761333727	31.661961764301523	20.049563027404087
13	31.317171715419402	21.18624257346088	29.396322499697497	18.10026321142222
14	20.554127483843658	23.322292823466196	29.193178274670593	26.93040141801955
15	22.8864695277157	29.1999873360133	27.736559585141674	20.17698355112933
16	21.303210971175986	28.412391450768098	28.0528122941289	22.231585283927018
17	18.57386161818861	27.45404859414568	28.577153387334537	25.39493640033117
18	19.68226404325532	26.542545140390267	33.70489701619993	20.070293800154484
19	18.927672589104375	24.79990250465033	35.54273640128531	20.729688504959988
20	23.91793389659161	23.520449519484107	33.97214183144667	18.589474752477607
21	23.12209774602722	24.52966517199819	32.53243737073084	19.815799711243756
22	21.15007214569136	24.288572356685524	33.463717461252315	21.097638036370796
23	20.14870643013925	26.66736347506734	34.612540556200564	18.57138953859285
24	18.60834062307682	25.604889686700776	32.775958895821795	23.01081079440061
25	19.780323200553745	26.836635872650604	34.29294837126819	19.09009255552746
26	21.475085557807414	25.42421102712305	32.61106684980295	20.48963656526659
27	20.003157322711775	27.182336687699582	32.08095757087387	20.73354841871477
28	19.66435230864044	26.650319136801848	32.19523703990587	21.490091514651844
29	18.632497611407302	26.534261505253603	33.33352126920903	21.49971961413006
30	17.82876815406347	26.828178758244057	35.839125733871526	19.503927353820945
31	19.451146285960625	26.03034759607607	34.3461631373032	20.172342980660098
32	20.821459038725344	26.51244648706647	31.86701426129708	20.799080212911107
33	21.857607347887956	26.4152980959349	31.289935470048587	20.437159086128553
34	19.893865382688762	29.57955997850592	31.309148299187555	19.217426339617766
35	18.20664937367478	30.618961023978734	31.53050784755162	19.643881754794858
36	19.456784362231655	26.28969910454338	34.22216882915805	20.031347704066917
37	19.528518040326123	26.78337773679812	32.91959973128061	20.768504491595145
38	19.889571770759286	26.301061996720378	33.6223208837381	20.18704534878224
39	21.144694288325148	25.573750157757715	33.360063597500336	19.921491956416805
40	19.059126505854273	26.583095919724204	32.525498199935726	21.832279374485797
41	20.226772101584256	25.961823284474345	32.28809181910796	21.52331279483344
42	17.379023146905283	27.876687465385462	32.68470879986607	22.05958058784318
43	18.158378766831284	27.64617688553534	32.310253795834846	21.885190551798527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	215.0
1	302.5
2	390.0
3	1143.0
4	1896.0
5	1896.0
6	3355.5
7	4815.0
8	5537.0
9	6259.0
10	9581.5
11	12904.0
12	12904.0
13	23448.5
14	33993.0
15	55598.5
16	77204.0
17	73251.0
18	69298.0
19	69298.0
20	73838.5
21	78379.0
22	61371.0
23	44363.0
24	47843.5
25	51324.0
26	51324.0
27	54596.5
28	57869.0
29	59257.0
30	60645.0
31	64128.0
32	67611.0
33	67611.0
34	73045.0
35	78479.0
36	86389.5
37	94300.0
38	98471.5
39	102643.0
40	102643.0
41	108097.0
42	113551.0
43	132790.0
44	152029.0
45	196565.5
46	241102.0
47	241102.0
48	246029.0
49	250956.0
50	224373.5
51	197791.0
52	180737.0
53	163683.0
54	163683.0
55	139450.0
56	115217.0
57	91318.0
58	67419.0
59	57940.5
60	48462.0
61	48462.0
62	42251.0
63	36040.0
64	31331.0
65	26622.0
66	22350.5
67	18079.0
68	18079.0
69	15243.5
70	12408.0
71	10476.0
72	8544.0
73	6772.0
74	5000.0
75	5000.0
76	3959.5
77	2919.0
78	2246.0
79	1573.0
80	1253.5
81	934.0
82	934.0
83	678.0
84	422.0
85	320.5
86	219.0
87	162.5
88	106.0
89	106.0
90	88.0
91	70.0
92	41.0
93	12.0
94	9.0
95	6.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2305751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.7745328420871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19677599315222	27.27199485846134
2	13.814036616569005	10.436375597087594
3	5.459110216837053	6.186460145234535
4	2.738018632419012	4.137094990102335
5	1.4854618764816203	2.805631421941165
6	0.9322647986543314	2.112952035257386
7	0.62601964703326	1.6553319801654753
8	0.4145457989228632	1.2527419116768745
9	0.31386207607037875	1.0670393970365544
>10	1.723282663716308	12.193691528949252
>50	0.16689336767971252	4.398860578770539
>100	0.10909941862566169	7.879134589056848
>500	0.00967343609387518	2.506885472826118
>1k	0.009207246402604086	6.986145924895728
>5k	0.0010489268053599592	2.972100231551726
>10k+	6.992845369066395E-4	6.137559336986567
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37192	1.6130102513237554	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34093	1.478607186985932	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29309	1.2711259802120871	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15938	0.6912281508280816	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12565	0.5449417565036294	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10313	0.44727292756242976	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9864	0.42779987951864706	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9464	0.4104519525308674	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8789	0.38117732573898916	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7599	0.32956724295034456	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6953	0.30155034086508037	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6700	0.29057777704530974	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6696	0.290404297775432	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5797	0.2514148318703971	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5647	0.24490935924997972	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4889	0.21203503760813722	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4789	0.20769805586119233	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4512	0.19568461642215487	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4180	0.18128583702229772	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4051	0.17569113056873878	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3664	0.15890701120806192	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3405	0.14767422848347458	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3362	0.14580932633228827	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3237	0.14038809914860712	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3230	0.14008451042632097	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3163	0.13717873265586789	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2765	0.11991754530302709	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2762	0.11978743585061874	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2704	0.11727198643739069	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2615	0.11341207268260971	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2520	0.10929194002301203	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2473	0.10725355860194792	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2388	0.10356712411704473	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2333	0.10118178415622502	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2308	0.1000975387194888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.336981746944922E-5	0.0
3	0.0	0.0	0.0	4.336981746944922E-5	0.0
4	0.0	0.0	0.0	4.336981746944922E-5	0.0
5	0.0	0.0	0.0	8.673963493889844E-5	0.0
6	0.0	0.0	0.0	8.673963493889844E-5	0.0
7	0.0	0.0	0.0	8.673963493889844E-5	0.0
8	4.336981746944922E-5	0.0	0.0	1.7347926987779688E-4	0.0
9	4.336981746944922E-5	0.0	0.0	2.602189048166953E-4	0.0
10	4.336981746944922E-5	0.0	0.0	3.4695853975559376E-4	0.0
11	4.336981746944922E-5	0.0	0.0	5.204378096333906E-4	0.0
12	4.336981746944922E-5	0.0	0.0	9.541359843278827E-4	0.0
13	4.336981746944922E-5	0.0	0.0	9.541359843278827E-4	0.0
14	4.336981746944922E-5	0.0	0.0	0.0012577247066140273	0.0
15	4.336981746944922E-5	0.0	0.0	0.0019516417861252147	0.0
16	4.336981746944922E-5	0.0	0.0	0.002862407952983648	0.0
17	4.336981746944922E-5	0.0	0.0	0.0047273101041699645	0.0
18	4.336981746944922E-5	0.0	0.0	0.005507966818620051	0.0
19	4.336981746944922E-5	0.0	0.0	0.006548842437886832	0.0
20	4.336981746944922E-5	0.0	0.0	0.007980046414378656	0.0
21	4.336981746944922E-5	0.0	0.0	0.010885824184831753	0.0
22	4.336981746944922E-5	0.0	0.0	0.016784119360676846	0.0
23	4.336981746944922E-5	0.0	0.0	0.026802547196119617	0.0
24	4.336981746944922E-5	0.0	0.0	0.04120132659597676	0.0
25	4.336981746944922E-5	0.0	0.0	0.049441591915172105	0.0
26	4.336981746944922E-5	0.0	0.0	0.061324921901801194	0.0
27	4.336981746944922E-5	0.0	0.0	0.14650324341179946	0.0
28	4.336981746944922E-5	0.0	0.0	0.22886252678628352	0.0
29	4.336981746944922E-5	0.0	0.0	0.32414601576666346	0.0
30	4.336981746944922E-5	0.0	0.0	0.5100724232581922	0.0
31	4.336981746944922E-5	0.0	0.0	0.7506014309437576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8400	0.0	29.159523	1
GTATTGG	755	0.0	23.52318	1
ATTGGAC	820	0.0	21.884146	3
CGCGATA	60	3.7283808E-5	21.583332	14
TAGGTCG	95	7.1540853E-9	21.421053	21
TTGGACC	1540	0.0	20.301947	4
TGCGCGA	65	6.905982E-5	19.923077	12
TATTGGA	995	0.0	19.708542	2
GGACCCT	1720	0.0	18.177324	6
ACCGACC	235	0.0	18.106384	8
TGGACCC	1750	0.0	18.077143	5
GTATCAA	14605	0.0	17.366312	2
CGAATTA	160	3.45608E-11	17.34375	15
CTGTGCG	140	1.87174E-9	17.178572	9
GTAATAC	155	4.0199666E-10	16.709679	3
CGACCGC	200	0.0	16.65	10
CGTCTGT	795	0.0	16.522013	34
CCGACAC	90	4.4490804E-5	16.444445	33
GACCCTC	1895	0.0	16.401056	7
CCGTCGA	80	3.3844108E-4	16.1875	9
>>END_MODULE