FastQCFastQC Report
Wed 25 May 2016
SRR2088265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940998
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128711.3678031196665668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121791.2942641748441548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101341.0769417150727207No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG51680.5492041428355852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.46089364695780444No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT36070.3833164363792484No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32620.3466532341195199No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT32060.3407021056367814No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30770.32699325609618723No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA27470.29192410610862085No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT24590.2613183024831084No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT23460.2493097753661538No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG21900.2327316317356679No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19980.2123277626519929No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA19330.2054202028059571No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT17020.18087179781466062No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.1768335320585166No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16440.17470812902896712No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15880.16875700054622858No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC13950.14824686131107612No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.1409142208591304No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12920.1373010357088963No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG11710.12444234738012196No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG11440.12157305329023016No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11110.11806613829147353No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC10590.11254009041464488No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC9910.10531372011417664No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA9500.10095664390360022No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.1002127528432579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.030.875861
ATTGGAC2200.026.909093
CGTCTGT3250.022.7692334
CATAGAC502.701597E-422.23
GCGTCTG3850.020.66233633
GCCTATG450.003825300420.5555551
TTGGACC3800.020.4473694
GTATAGA555.1421433E-420.1818181
GGACCCT3650.019.7671226
GTCTTAC759.262843E-619.7333341
GTATTGG2350.019.6808511
TGCGTCT4100.018.95122132
GTATCAA49800.018.5742972
GCAAGGT500.007033956218.53
ACGATCT902.1513715E-618.532
TGCGACG500.007033956218.522
CGATCTG902.1513715E-618.533
GACCCTC4100.018.0487827
TACTAGG953.6049805E-617.5263162
CCGAATT752.0667646E-417.26666614
Produced by FastQC (version 0.11.5)