##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	940998
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.954859627756914	31.0	30.0	33.0	27.0	34.0
2	30.780076046920396	31.0	31.0	34.0	27.0	34.0
3	30.752541450672584	31.0	31.0	34.0	27.0	34.0
4	34.95016673786767	35.0	35.0	37.0	32.0	37.0
5	34.84890403592781	35.0	35.0	37.0	32.0	37.0
6	34.965113634672974	35.0	35.0	37.0	32.0	37.0
7	34.56899695854826	35.0	35.0	37.0	30.0	37.0
8	34.79525354995441	35.0	35.0	37.0	32.0	37.0
9	36.19853921049779	38.0	35.0	39.0	32.0	39.0
10	36.12936478079656	38.0	35.0	39.0	31.0	39.0
11	36.23154565684518	38.0	35.0	39.0	32.0	39.0
12	35.9576853510847	37.0	35.0	39.0	30.0	39.0
13	36.13554013929892	38.0	35.0	39.0	31.0	39.0
14	37.00542934203899	39.0	36.0	40.0	31.0	41.0
15	37.05611807889071	38.0	36.0	40.0	31.0	41.0
16	36.969740637068305	38.0	36.0	40.0	31.0	41.0
17	37.08009368776554	39.0	36.0	40.0	31.0	41.0
18	37.097193617839785	39.0	36.0	40.0	31.0	41.0
19	37.19344568213747	39.0	36.0	40.0	31.0	41.0
20	37.15303964514271	39.0	36.0	40.0	31.0	41.0
21	37.09258361866869	39.0	36.0	40.0	31.0	41.0
22	37.02429760743381	39.0	36.0	40.0	31.0	41.0
23	36.893928573705786	39.0	35.0	40.0	31.0	41.0
24	36.82907190025909	39.0	35.0	40.0	31.0	41.0
25	36.73156372277093	38.0	35.0	40.0	30.0	41.0
26	36.46376825455527	38.0	35.0	40.0	30.0	41.0
27	36.3111271224806	38.0	35.0	40.0	30.0	41.0
28	36.140213900560894	38.0	35.0	40.0	30.0	41.0
29	35.981706656124665	38.0	34.0	40.0	29.0	41.0
30	35.7968348498084	38.0	34.0	40.0	29.0	41.0
31	35.528661059853476	38.0	34.0	40.0	28.0	41.0
32	35.25892616137335	37.0	34.0	40.0	27.0	41.0
33	35.376414190040784	38.0	34.0	40.0	27.0	41.0
34	35.35529618553918	38.0	34.0	40.0	27.0	41.0
35	35.26185071594201	38.0	34.0	40.0	27.0	41.0
36	35.165353167594404	38.0	34.0	40.0	26.0	41.0
37	35.002168973791655	38.0	33.0	40.0	25.0	41.0
38	34.87296784902837	38.0	33.0	40.0	25.0	41.0
39	34.807055912977496	38.0	33.0	40.0	24.0	41.0
40	34.63711718834684	38.0	33.0	40.0	24.0	41.0
41	34.44477246497867	38.0	33.0	40.0	23.0	41.0
42	34.233912293118586	38.0	33.0	40.0	22.0	41.0
43	33.88261186527495	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	4.0
16	9.0
17	31.0
18	83.0
19	166.0
20	416.0
21	780.0
22	1343.0
23	2503.0
24	4084.0
25	6379.0
26	9777.0
27	13842.0
28	19340.0
29	26252.0
30	33403.0
31	41286.0
32	50137.0
33	60668.0
34	72869.0
35	86437.0
36	104496.0
37	128942.0
38	145707.0
39	132040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.651516793872034	25.558927861695775	14.161454115736696	21.628101228695492
2	17.998125394527936	23.619391327080397	35.67754660477493	22.704936673616736
3	19.840531010692903	26.207282055859842	32.72568060718514	21.22650632626212
4	13.356351448143355	16.409599170242657	35.159798426776675	35.07425095483731
5	11.126166049237087	40.74982093479476	34.66107260589289	13.46294041007526
6	31.489865015653596	36.273509614260604	15.19833198370241	17.038293386383394
7	23.83894546003286	35.931851077260525	21.459237957997786	18.76996550470883
8	27.438953111483766	33.87456721480811	20.232561599493305	18.453918074214823
9	24.94585535782223	13.939349499148776	24.267426710790033	36.847368432238966
10	16.336060225420248	28.611325422583256	33.884981689652896	21.167632662343596
11	31.138642165020542	22.11503106276528	25.047768433089125	21.698558339125057
12	19.938937170961044	28.262440515282712	31.630035345452384	20.168586968303863
13	30.873179326629813	21.530438959487693	28.840868949774602	18.75551276410789
14	20.566568685587004	23.42746743351208	28.903249528691877	27.10271435220904
15	22.856796720078044	29.666375486451617	26.8830539491051	20.593773844365238
16	21.449036023455946	28.741825168597597	27.600483741729526	22.20865506621693
17	18.93404661859005	27.979124291443764	28.400166631597518	24.68666245836867
18	19.942019005353888	26.913553482579132	32.90974050954412	20.234687002522854
19	19.367947647072576	25.602179813347103	34.34555652615627	20.684316013424045
20	23.535862988019105	24.477310259958042	33.09231262978242	18.89451412224043
21	23.00376833957139	25.29877853087892	31.700492455881946	19.996960673667743
22	21.305783859264313	25.16530322062321	32.43322515031913	21.095687769793347
23	20.444889362145297	27.296338568200994	33.251930397301585	19.00684167235212
24	19.110136259588227	26.304519244461734	31.96606156442415	22.61928293152589
25	20.25966048812006	27.101439110391308	33.19454451550376	19.44435588598488
26	21.445529108457194	26.19027883162345	31.64193760241786	20.722254457501503
27	20.36061713202366	27.492406997676934	31.316007047836447	20.830968822462957
28	20.035961819259978	27.200057810962402	31.390077343416245	21.373903026361376
29	19.11364317458698	27.007496296485222	32.32823024065938	21.55063028826841
30	18.58771219492496	27.1623319071879	34.38147583735566	19.86848006053148
31	19.95625920565187	26.54341454498309	33.148954620519916	20.35137162884512
32	20.844146321246164	27.053617542226444	31.2784936843649	20.82374245216249
33	21.87964267724267	26.900694794250356	30.661807995341118	20.55785453316585
34	20.09727969666248	29.517809814686107	30.792307741355458	19.592602747295956
35	18.881549163760177	30.286674360625632	30.95521988357042	19.876556592043766
36	19.848501272053714	26.755104686726224	33.05033591994882	20.346058121271245
37	19.80578067115977	27.103564513420856	32.2550101062914	20.83564470912797
38	20.199405312232333	26.718122674012058	32.69071772734905	20.39175428640656
39	21.2558368880699	26.12311609588968	32.652779283271585	19.968267732768826
40	19.517363480049905	26.931194327724395	31.86871810567079	21.682724086554913
41	20.20705676313871	26.31790928354789	31.79974877736191	21.67528517595149
42	17.86294976184859	27.97359824356694	32.28816639355237	21.875285601032097
43	18.50482147677253	27.73023959668352	31.833861495986177	21.931077430557767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	180.5
2	200.0
3	458.5
4	717.0
5	717.0
6	1247.5
7	1778.0
8	2011.0
9	2244.0
10	3387.5
11	4531.0
12	4531.0
13	8163.5
14	11796.0
15	19482.5
16	27169.0
17	26052.5
18	24936.0
19	24936.0
20	27068.0
21	29200.0
22	24128.5
23	19057.0
24	20341.5
25	21626.0
26	21626.0
27	24076.5
28	26527.0
29	27429.5
30	28332.0
31	30485.5
32	32639.0
33	32639.0
34	34885.5
35	37132.0
36	39389.0
37	41646.0
38	43147.0
39	44648.0
40	44648.0
41	46310.0
42	47972.0
43	54220.5
44	60469.0
45	76120.5
46	91772.0
47	91772.0
48	93611.0
49	95450.0
50	85908.0
51	76366.0
52	70116.5
53	63867.0
54	63867.0
55	55445.0
56	47023.0
57	38531.5
58	30040.0
59	26148.0
60	22256.0
61	22256.0
62	19444.0
63	16632.0
64	14208.0
65	11784.0
66	10045.0
67	8306.0
68	8306.0
69	6911.5
70	5517.0
71	4742.5
72	3968.0
73	3129.5
74	2291.0
75	2291.0
76	1843.5
77	1396.0
78	1088.0
79	780.0
80	598.0
81	416.0
82	416.0
83	296.0
84	176.0
85	131.0
86	86.0
87	72.0
88	58.0
89	58.0
90	40.5
91	23.0
92	15.5
93	8.0
94	4.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	940998.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.47549071241427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89609172044156	37.275757800152206
2	12.737284288713907	12.348922124778626
3	4.386734979592402	6.37947392283163
4	1.9880231849814773	3.8548159775853534
5	1.0447268142008308	2.5321822489401264
6	0.646269991911778	1.8796952994378866
7	0.42062507826604006	1.4273004952425765
8	0.29846121213825855	1.1574442973619248
9	0.1981770548845433	0.8646056985122363
>10	1.2144985763176595	10.867109437508624
>50	0.09476313402085718	3.2265385533321043
>100	0.05925324935594048	5.633962999879321
>500	0.009320735843211322	3.234867909780893
>1k	0.004882290203586883	4.976339200886083
>5k	2.2192228198122192E-4	0.5559626657049436
>10k+	6.657668459436658E-4	3.7850213680655442
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12871	1.3678031196665668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12179	1.2942641748441548	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10134	1.0769417150727207	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5168	0.5492041428355852	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4337	0.46089364695780444	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3607	0.3833164363792484	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3262	0.3466532341195199	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3206	0.3407021056367814	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3077	0.32699325609618723	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2747	0.29192410610862085	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2459	0.2613183024831084	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2346	0.2493097753661538	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2190	0.2327316317356679	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1998	0.2123277626519929	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1933	0.2054202028059571	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1702	0.18087179781466062	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1664	0.1768335320585166	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1644	0.17470812902896712	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1588	0.16875700054622858	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1395	0.14824686131107612	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1326	0.1409142208591304	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1292	0.1373010357088963	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1171	0.12444234738012196	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1144	0.12157305329023016	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1111	0.11806613829147353	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1059	0.11254009041464488	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	991	0.10531372011417664	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	950	0.10095664390360022	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943	0.1002127528432579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.1254030295494783E-4	0.0
10	0.0	0.0	0.0	2.1254030295494783E-4	0.0
11	0.0	0.0	0.0	4.2508060590989565E-4	0.0
12	0.0	0.0	0.0	7.438910603423174E-4	0.0
13	0.0	0.0	0.0	8.501612118197913E-4	0.0
14	0.0	0.0	0.0	9.564313632972652E-4	0.0
15	0.0	0.0	0.0	0.001275241817729687	0.0
16	0.0	0.0	0.0	0.0024442134839819	0.0
17	0.0	0.0	0.0	0.003825725453189061	0.0
18	0.0	0.0	0.0	0.004569616513531378	0.0
19	0.0	0.0	0.0	0.005100967270918748	0.0
20	0.0	0.0	0.0	0.005526047876828644	0.0
21	0.0	0.0	0.0	0.007863991209333069	0.0
22	0.0	0.0	0.0	0.013708849540594136	0.0
23	0.0	0.0	0.0	0.021572840749927207	0.0
24	0.0	0.0	0.0	0.03411271862426913	0.0
25	0.0	0.0	0.0	0.0415516292276923	0.0
26	0.0	0.0	0.0	0.05143475331509738	0.0
27	0.0	0.0	0.0	0.11891629950329331	0.0
28	0.0	0.0	0.0	0.1919238935683179	0.0
29	0.0	0.0	0.0	0.2692885638439189	0.0
30	0.0	0.0	0.0	0.4286937910601298	0.0
31	0.0	0.0	0.0	0.6428281462872397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2900	0.0	30.87586	1
ATTGGAC	220	0.0	26.90909	3
CGTCTGT	325	0.0	22.76923	34
CATAGAC	50	2.701597E-4	22.2	3
GCGTCTG	385	0.0	20.662336	33
GCCTATG	45	0.0038253004	20.555555	1
TTGGACC	380	0.0	20.447369	4
GTATAGA	55	5.1421433E-4	20.181818	1
GGACCCT	365	0.0	19.767122	6
GTCTTAC	75	9.262843E-6	19.733334	1
GTATTGG	235	0.0	19.680851	1
TGCGTCT	410	0.0	18.951221	32
GTATCAA	4980	0.0	18.574297	2
GCAAGGT	50	0.0070339562	18.5	3
ACGATCT	90	2.1513715E-6	18.5	32
TGCGACG	50	0.0070339562	18.5	22
CGATCTG	90	2.1513715E-6	18.5	33
GACCCTC	410	0.0	18.048782	7
TACTAGG	95	3.6049805E-6	17.526316	2
CCGAATT	75	2.0667646E-4	17.266666	14
>>END_MODULE