FastQCFastQC Report
Wed 25 May 2016
SRR2088264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553042
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75151.3588479717634465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73961.3373306186510245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61731.1161900904452102No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG33860.6122500641904232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.45240686964100374No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT20930.37845226944789007No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20370.368326456218515No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT19080.34500092217227624No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA18760.339214743184062No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA15880.28713913229013344No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT15800.2856925875430799No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT14220.2571233287887719No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG13770.2489865145865956No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12800.23144715952857106No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11900.21517353112421841No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT10780.19492190466546847No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA9740.17611682295377204No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC9280.16779919065821403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9090.1643636468839618No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8870.1603856488295645No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7400.13380538910245515No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG6920.12512612062013373No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC6710.1213289406591181No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6710.1213289406591181No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT6610.11952075972530116No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6390.11554276167090383No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC5610.10143895038713155No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.10017322373345966No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTTA303.597084E-430.83333426
GGTATCA17750.028.1408441
GCCGTAC358.8635506E-426.4285721
TATACTG551.9003452E-523.5454545
AGCCGTC400.00192992723.12512
GTATTGG1450.021.6896551
GCCGTCA603.7217862E-521.58333413
ATTGGAC1600.020.81253
CGGGATA450.003823336220.55555524
TACCGTC555.1384576E-420.1818187
TTGGACC3100.019.0967754
GGACCCT2850.018.8245626
ACCGACG902.14811E-618.58
GTGATAC500.00703037918.53
TCTAGAC500.00703037918.53
ATTCCGT500.00703037918.529
TAGATTG609.228554E-418.55
ACACTTA609.228554E-418.56
TATCCCG500.00703037918.55
TCACGTT500.00703037918.524
Produced by FastQC (version 0.11.5)