##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	553042
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.982030297879728	31.0	30.0	33.0	27.0	34.0
2	30.826566517552013	31.0	31.0	34.0	27.0	34.0
3	30.82226847147233	31.0	31.0	34.0	27.0	34.0
4	35.00941881448425	35.0	35.0	37.0	32.0	37.0
5	34.9310721428029	35.0	35.0	37.0	32.0	37.0
6	35.03220008606941	36.0	35.0	37.0	32.0	37.0
7	34.63464076869388	35.0	35.0	37.0	30.0	37.0
8	34.86021314836848	35.0	35.0	37.0	32.0	37.0
9	36.27820491029615	38.0	35.0	39.0	32.0	39.0
10	36.2187175657545	38.0	35.0	39.0	32.0	39.0
11	36.31593983820397	38.0	35.0	39.0	32.0	39.0
12	36.04758228127339	38.0	35.0	39.0	30.0	39.0
13	36.211378159344136	38.0	35.0	39.0	32.0	39.0
14	37.09478665273162	39.0	36.0	40.0	31.0	41.0
15	37.13407480806159	39.0	36.0	40.0	31.0	41.0
16	37.06676527280026	39.0	36.0	40.0	31.0	41.0
17	37.16804329508428	39.0	36.0	40.0	31.0	41.0
18	37.19451868031723	39.0	36.0	40.0	32.0	41.0
19	37.271299467309895	39.0	36.0	40.0	31.0	41.0
20	37.23661855699929	39.0	36.0	40.0	31.0	41.0
21	37.17119495445192	39.0	36.0	40.0	31.0	41.0
22	37.100650583499984	39.0	36.0	40.0	31.0	41.0
23	36.96845628360956	39.0	35.0	40.0	31.0	41.0
24	36.88974435937958	39.0	35.0	40.0	31.0	41.0
25	36.80052871210505	39.0	35.0	40.0	30.0	41.0
26	36.544108765699534	38.0	35.0	40.0	30.0	41.0
27	36.399777955381325	38.0	35.0	40.0	30.0	41.0
28	36.24747487532593	38.0	35.0	40.0	30.0	41.0
29	36.057648424531955	38.0	35.0	40.0	30.0	41.0
30	35.87883379562493	38.0	34.0	40.0	29.0	41.0
31	35.60591781456019	38.0	34.0	40.0	28.0	41.0
32	35.355508261578684	38.0	34.0	40.0	27.0	41.0
33	35.47625135161525	38.0	34.0	40.0	28.0	41.0
34	35.45343210823048	38.0	34.0	40.0	27.0	41.0
35	35.34951775814495	38.0	34.0	40.0	27.0	41.0
36	35.24282604214508	38.0	34.0	40.0	26.0	41.0
37	35.07779698467748	38.0	33.0	40.0	26.0	41.0
38	34.93545336520554	38.0	33.0	40.0	25.0	41.0
39	34.88259300378633	38.0	33.0	40.0	25.0	41.0
40	34.70595180836175	38.0	33.0	40.0	24.0	41.0
41	34.52257694713964	38.0	33.0	40.0	23.0	41.0
42	34.30100788005251	38.0	33.0	40.0	22.0	41.0
43	33.94493004147967	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	7.0
17	12.0
18	44.0
19	99.0
20	228.0
21	445.0
22	758.0
23	1338.0
24	2273.0
25	3617.0
26	5481.0
27	8043.0
28	11117.0
29	14779.0
30	19221.0
31	23361.0
32	28907.0
33	34736.0
34	42366.0
35	50315.0
36	60889.0
37	77350.0
38	87286.0
39	80369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23499481052072	25.212551668770182	13.901837473464946	21.65061604724415
2	17.89068461346516	23.108733152274148	36.11570188159308	22.884880352667608
3	19.68259191887777	26.547712470300628	32.49915919586577	21.270536414955828
4	12.8907026952745	15.931158935487721	35.2797798358895	35.89835853334828
5	10.981263629163788	40.78388259842833	34.69682230282691	13.538031469580972
6	31.54950256942511	36.084601169531425	15.301550334332656	17.06434592671081
7	23.924584389612363	35.765818870899494	21.46943631767569	18.84016042181245
8	27.926631250429445	33.4442953699719	20.111673254472535	18.51740012512612
9	24.96790478842475	13.631876060046071	24.09401094311101	37.30620820841817
10	16.45950940434904	28.183754579218217	33.53380032619584	21.822935690236907
11	31.33776458207514	21.98440624762676	25.061206924609703	21.616622245688394
12	20.104982985017415	28.088825080192827	31.60121654413227	20.20497539065749
13	31.06870725912318	21.014317176633963	28.952412294183805	18.964563270059053
14	20.87635297138373	22.90386625247269	28.7894951920469	27.430285584096687
15	22.961547224261448	29.357806459545564	27.013319060758494	20.667327255434486
16	21.76453144607462	28.439792999446695	27.461747932345105	22.333927622133583
17	19.101080930562237	27.65630820082381	28.08159235645756	25.161018512156403
18	20.184904582292123	26.627091613295196	32.653035393333596	20.534968411079085
19	19.496530100788007	25.292111629858134	34.304808676375394	20.90654959297847
20	23.969608094864405	24.010111347781905	32.84108621045056	19.179194346903127
21	23.340722766082866	24.97115951410562	31.530155033433267	20.15796268637825
22	21.53742392078721	24.71656763862419	32.34238990890385	21.403618531684753
23	20.495550066721876	26.786392353564466	33.53597014331642	19.182087436397236
24	19.064555675699133	26.162389113304236	31.79903153829185	22.974023672704785
25	20.38814411925315	26.860889408037725	33.10309162776064	19.647874844948486
26	21.75422481475186	25.859699624983275	31.653834609306347	20.732240950958516
27	20.45757826711172	27.434263582151086	31.13145113752663	20.976707013210568
28	20.068819366341074	26.86685640511932	31.405752185186657	21.658572043352947
29	19.21644287413976	26.821290245587136	32.37981925423385	21.582447626039254
30	18.579420731156045	27.00897942651734	34.405162718202234	20.00643712412439
31	20.02867774961034	26.349897476141056	33.193139038264725	20.428285735983888
32	21.17289464452971	26.766140727105718	30.962928674494883	21.098035953869687
33	21.995436151323048	26.80501661718278	30.428249572365207	20.77129765912896
34	20.20913420680527	29.47298758502971	30.579051862245542	19.738826345919477
35	18.886992307998305	30.35375251789195	30.739979965355253	20.01927520875449
36	19.894329906227735	26.78259517360345	32.863869290216655	20.459205629952155
37	19.94260833716065	27.08709284285823	31.906799121947337	21.063499698033784
38	20.34981068345623	26.72563747418822	32.47275975423205	20.451792088123504
39	21.28699086145356	26.131830855522725	32.290133479916534	20.291044803107177
40	19.492732920826988	26.99559888760709	31.646240249384316	21.865427942181608
41	20.587948112439925	26.465801873998718	31.413888999388835	21.532361014172523
42	18.04618817377342	28.00872266482473	31.96520336610963	21.97988579529222
43	18.646504243800653	27.766968873973408	31.535398758141337	22.05112812408461
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	74.0
2	94.0
3	265.0
4	436.0
5	436.0
6	768.0
7	1100.0
8	1174.0
9	1248.0
10	1910.5
11	2573.0
12	2573.0
13	4715.5
14	6858.0
15	11529.0
16	16200.0
17	15486.5
18	14773.0
19	14773.0
20	15720.0
21	16667.0
22	13479.0
23	10291.0
24	11178.0
25	12065.0
26	12065.0
27	13015.0
28	13965.0
29	14449.0
30	14933.0
31	16063.5
32	17194.0
33	17194.0
34	18623.5
35	20053.0
36	21988.5
37	23924.0
38	24903.5
39	25883.0
40	25883.0
41	27254.5
42	28626.0
43	32859.0
44	37092.0
45	46606.0
46	56120.0
47	56120.0
48	57188.5
49	58257.0
50	52543.0
51	46829.0
52	42973.0
53	39117.0
54	39117.0
55	33630.0
56	28143.0
57	22821.0
58	17499.0
59	15138.5
60	12778.0
61	12778.0
62	11266.0
63	9754.0
64	8409.5
65	7065.0
66	6016.0
67	4967.0
68	4967.0
69	4198.5
70	3430.0
71	2813.0
72	2196.0
73	1724.5
74	1253.0
75	1253.0
76	970.5
77	688.0
78	565.5
79	443.0
80	342.5
81	242.0
82	242.0
83	184.0
84	126.0
85	86.0
86	46.0
87	37.0
88	28.0
89	28.0
90	28.0
91	28.0
92	15.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	553042.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.60777546461378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.77571065065133	45.20197421134608
2	9.870764688447725	10.780410035415835
3	2.933366277732339	4.805538211496323
4	1.2520697092938542	2.734909662046521
5	0.6924389496993288	1.890627534406697
6	0.4866213030344042	1.5943984111440317
7	0.3152559000192879	1.2050796381503601
8	0.24677014648649487	1.078045500056349
9	0.19374491684754364	0.9521981024958835
>10	1.103614900089217	10.873235091103137
>50	0.06934369389891556	2.6318513436260482
>100	0.04823909103964727	5.723778147713441
>500	0.006699873755506565	2.4055637010588944
>1k	0.004354917941079266	4.265439453809144
>5k	0.0010049810633259847	3.8569509561312345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7515	1.3588479717634465	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7396	1.3373306186510245	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6173	1.1161900904452102	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3386	0.6122500641904232	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2502	0.45240686964100374	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2093	0.37845226944789007	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2037	0.368326456218515	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1908	0.34500092217227624	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1876	0.339214743184062	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1588	0.28713913229013344	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1580	0.2856925875430799	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1422	0.2571233287887719	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1377	0.2489865145865956	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1280	0.23144715952857106	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1190	0.21517353112421841	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1078	0.19492190466546847	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	974	0.17611682295377204	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	928	0.16779919065821403	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	909	0.1643636468839618	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	887	0.1603856488295645	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	740	0.13380538910245515	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	692	0.12512612062013373	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	671	0.1213289406591181	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	671	0.1213289406591181	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	661	0.11952075972530116	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	639	0.11554276167090383	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	561	0.10143895038713155	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	554	0.10017322373345966	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.8081809338169614E-4	0.0
10	0.0	0.0	0.0	1.8081809338169614E-4	0.0
11	0.0	0.0	0.0	1.8081809338169614E-4	0.0
12	0.0	0.0	0.0	3.6163618676339227E-4	0.0
13	0.0	0.0	0.0	3.6163618676339227E-4	0.0
14	0.0	0.0	0.0	9.040904669084807E-4	0.0
15	0.0	0.0	0.0	0.0018081809338169614	0.0
16	0.0	0.0	0.0	0.0030739075874888343	0.0
17	0.0	0.0	0.0	0.005605360894832581	0.0
18	0.0	0.0	0.0	0.005786178988214276	0.0
19	0.0	0.0	0.0	0.006871087548504453	0.0
20	0.0	0.0	0.0	0.007955996108794631	0.0
21	0.0	0.0	0.0	0.011210721789665161	0.0
22	0.0	0.0	0.0	0.018805081711696398	0.0
23	0.0	0.0	0.0	0.028026804474162902	0.0
24	0.0	0.0	0.0	0.045746977625569125	0.0
25	0.0	0.0	0.0	0.05279888326745528	0.0
26	0.0	0.0	0.0	0.06491369552402891	0.0
27	0.0	0.0	0.0	0.13452866147598194	0.0
28	0.0	0.0	0.0	0.2017929922139729	0.0
29	0.0	0.0	0.0	0.2764708647806134	0.0
30	0.0	0.0	0.0	0.427273154660948	0.0
31	0.0	0.0	0.0	0.6310551459021195	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTA	30	3.597084E-4	30.833334	26
GGTATCA	1775	0.0	28.140844	1
GCCGTAC	35	8.8635506E-4	26.428572	1
TATACTG	55	1.9003452E-5	23.545454	5
AGCCGTC	40	0.001929927	23.125	12
GTATTGG	145	0.0	21.689655	1
GCCGTCA	60	3.7217862E-5	21.583334	13
ATTGGAC	160	0.0	20.8125	3
CGGGATA	45	0.0038233362	20.555555	24
TACCGTC	55	5.1384576E-4	20.181818	7
TTGGACC	310	0.0	19.096775	4
GGACCCT	285	0.0	18.824562	6
ACCGACG	90	2.14811E-6	18.5	8
GTGATAC	50	0.007030379	18.5	3
TCTAGAC	50	0.007030379	18.5	3
ATTCCGT	50	0.007030379	18.5	29
TAGATTG	60	9.228554E-4	18.5	5
ACACTTA	60	9.228554E-4	18.5	6
TATCCCG	50	0.007030379	18.5	5
TCACGTT	50	0.007030379	18.5	24
>>END_MODULE