FastQCFastQC Report
Wed 25 May 2016
SRR2088263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249401
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129981.0403385302236832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123530.9887137916489581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98240.7862967934234084No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45200.36177336179497216No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35490.2840561196925567No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT26940.21562332669815376No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT22350.17888572203800063No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA19400.15527440749607213No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA19370.15503429243293387No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT18960.1517527199033777No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT17170.13742585446946176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.13270359156107606No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG15800.12646059991948141No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14470.11581549878701873No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.11541530701512165No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT13130.10509035930017663No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12960.10372970727572653No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12620.1010084032268263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.025.2148131
ATCTGCG1150.024.13043236
TACGACG400.001931280223.1250025
TCACGTT1155.456968E-1222.52173824
GCCGGAA1350.021.92592415
CCCGTAT603.7266214E-521.5833321
ATCTACG450.003825990720.5555571
CGCATTG909.480027E-820.55555714
CGCCGGA1500.019.73333414
TAAACGT1002.875422E-718.4999984
CAATGCG752.0674472E-417.26666619
CCGGTCC752.0674472E-417.2666669
TGGCGTA650.001580038217.07692324
ATCACGT1301.3933459E-817.07692323
ACGCTAT650.001580038217.07692322
GTATCAA52750.016.7639792
TATACCG1901.8189894E-1216.5526315
CGTCGCA904.4464534E-516.44444511
ACGTTTA1352.2197128E-816.44444326
CTGTGCG1251.6579361E-716.2799999
Produced by FastQC (version 0.11.5)