##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1249401
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98020011189362	31.0	30.0	33.0	27.0	34.0
2	30.83876673702038	31.0	31.0	34.0	27.0	34.0
3	30.866533642921688	31.0	31.0	34.0	27.0	34.0
4	35.02876418379687	35.0	35.0	37.0	32.0	37.0
5	34.90974234853342	35.0	35.0	37.0	32.0	37.0
6	35.00599487274302	36.0	35.0	37.0	32.0	37.0
7	34.65470093268694	35.0	35.0	37.0	31.0	37.0
8	34.84575248459062	35.0	35.0	37.0	32.0	37.0
9	36.30185264778882	38.0	35.0	39.0	32.0	39.0
10	36.209074588542826	38.0	35.0	39.0	32.0	39.0
11	36.30276348426166	38.0	35.0	39.0	32.0	39.0
12	36.05041055673879	38.0	35.0	39.0	30.0	39.0
13	36.21777395728033	38.0	35.0	39.0	32.0	39.0
14	37.14200244757288	39.0	36.0	40.0	31.0	41.0
15	37.194521214566024	39.0	36.0	40.0	32.0	41.0
16	37.11539609781007	39.0	36.0	40.0	31.0	41.0
17	37.18589147919683	39.0	36.0	40.0	31.0	41.0
18	37.191648638027345	39.0	36.0	40.0	31.0	41.0
19	37.266075503381224	39.0	36.0	40.0	31.0	41.0
20	37.22454360129374	39.0	36.0	40.0	31.0	41.0
21	37.16481337857101	39.0	36.0	40.0	31.0	41.0
22	37.083600861532844	39.0	36.0	40.0	31.0	41.0
23	36.97519771474491	39.0	36.0	40.0	31.0	41.0
24	36.89867144335565	39.0	35.0	40.0	31.0	41.0
25	36.80572370279838	39.0	35.0	40.0	30.0	41.0
26	36.565915986940944	38.0	35.0	40.0	30.0	41.0
27	36.411020160861085	38.0	35.0	40.0	30.0	41.0
28	36.27549601769168	38.0	35.0	40.0	30.0	41.0
29	36.10944284501133	38.0	35.0	40.0	30.0	41.0
30	35.95195617740021	38.0	34.0	40.0	29.0	41.0
31	35.729359909268524	38.0	34.0	40.0	29.0	41.0
32	35.46392551310588	38.0	34.0	40.0	27.0	41.0
33	35.62088872987936	38.0	34.0	40.0	28.0	41.0
34	35.63016437476839	38.0	34.0	40.0	28.0	41.0
35	35.55506278608709	38.0	34.0	40.0	27.0	41.0
36	35.469800328317326	38.0	34.0	40.0	27.0	41.0
37	35.321495660720615	38.0	34.0	40.0	27.0	41.0
38	35.19707203692009	38.0	34.0	40.0	26.0	41.0
39	35.17722732733526	38.0	34.0	40.0	26.0	41.0
40	35.05803741152761	38.0	34.0	40.0	25.0	41.0
41	34.907661351319554	38.0	33.0	40.0	24.0	41.0
42	34.75466883730684	38.0	33.0	40.0	24.0	41.0
43	34.40146998441653	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	7.0
16	16.0
17	36.0
18	96.0
19	211.0
20	428.0
21	961.0
22	1713.0
23	2954.0
24	4799.0
25	7743.0
26	11558.0
27	17342.0
28	24097.0
29	32149.0
30	41505.0
31	52000.0
32	63628.0
33	77821.0
34	94075.0
35	112715.0
36	137855.0
37	171408.0
38	201569.0
39	192714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.695422846628105	25.383763899660718	13.616124847026695	22.304688406684484
2	17.835346698137748	23.729611229701273	35.494689054995156	22.940353017165826
3	19.31365510352561	26.65413266037085	32.2581781189546	21.77403411714894
4	13.47085523382805	17.144695738197743	36.304837278023626	33.07961174995058
5	11.62060859563903	40.25032795715707	34.91793267333706	13.211130773866836
6	30.78435186141199	37.60169873403335	15.91858818745943	15.695361217095233
7	24.117076903252038	35.01117735618909	22.75946633626834	18.112279404290536
8	26.453876697713547	34.50197334562722	20.700319593149036	18.343830363510193
9	25.072654816187917	14.194161842354857	23.81821368799929	36.91496965345794
10	16.07410271001864	28.593221871921028	34.572807289253014	20.759868128807323
11	31.450431046557508	23.294442696940372	24.397131105225625	20.857995151276494
12	19.53376057806901	28.51334359425036	31.692386991846494	20.26050883583413
13	29.42986279024909	22.1039522139009	28.228567129368393	20.23761786648162
14	20.534560161229262	23.54264163387095	28.913375289438697	27.00942291546109
15	22.942353976025313	30.085456950970908	26.069452481629195	20.902736591374588
16	21.403856728144127	28.74297363296492	27.860470737577447	21.99269890131351
17	19.43467309534729	28.73561010436201	28.672699957819788	23.157016842470913
18	19.99790299511526	27.688708429079217	31.5698482712916	20.743540304513925
19	19.904098043782582	26.962360363085992	33.14692400598367	19.986617587147762
20	22.405216579785034	26.141086808798775	32.00085480962478	19.45284180179142
21	21.995980473843066	26.680705394024816	31.054321230733766	20.26899290139835
22	21.36639877829456	26.59578470002825	31.52486671613037	20.512949805546818
23	20.64373247660279	27.75425984131596	31.74881403168398	19.853193650397273
24	19.639571282558602	27.548081040434575	31.10170393652638	21.710643740480435
25	20.563614083868988	27.914256511720414	31.74937430016464	19.772755104245952
26	21.07746031898486	27.431785311521278	30.85998810630054	20.630766263193323
27	20.390090931574413	28.130520145253605	30.709516000067232	20.769872923104753
28	20.16438277222445	28.274989374908454	30.752016366242703	20.80861148662439
29	19.556331394004005	27.944350932967076	31.540154041816837	20.95916363121208
30	19.288603098604852	28.333817565377327	32.51614173511947	19.861437600898352
31	20.357835474759504	27.84734444745922	31.667415025280114	20.127405052501157
32	20.62180196750283	28.27586979680663	30.756258399024812	20.34606983666573
33	21.117559534528947	28.21007826950675	30.229606027208238	20.44275616875607
34	20.23153495154878	29.155971541562714	30.55712297332882	20.05537053355968
35	19.563534845898154	29.467961046933688	30.776908294454703	20.19159581271345
36	20.105954773527472	27.662055657070866	31.643163403903152	20.588826165498507
37	19.99958380055723	27.48172924465404	31.48756884298956	21.031118111799174
38	20.050728309005674	27.5476008103083	31.672217326542878	20.729453554143145
39	20.627244575600628	27.02871215886653	31.903127978927504	20.44091528660534
40	19.62196284459513	27.211439721914743	31.732966437516858	21.43363099597327
41	19.875924543041027	26.742975233732004	31.69830983007057	21.682790393156402
42	18.53136022782117	27.809486305837755	31.624034237206473	22.0351192291346
43	18.793886030185664	27.373677466241826	31.74921422345588	22.083222280116633
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	203.0
1	244.0
2	285.0
3	665.0
4	1045.0
5	1045.0
6	1696.5
7	2348.0
8	2630.0
9	2912.0
10	4310.0
11	5708.0
12	5708.0
13	9899.0
14	14090.0
15	21847.0
16	29604.0
17	28820.0
18	28036.0
19	28036.0
20	30934.0
21	33832.0
22	29308.0
23	24784.0
24	27964.0
25	31144.0
26	31144.0
27	34407.5
28	37671.0
29	40392.5
30	43114.0
31	46199.5
32	49285.0
33	49285.0
34	53897.5
35	58510.0
36	62652.5
37	66795.0
38	69438.0
39	72081.0
40	72081.0
41	74336.5
42	76592.0
43	82350.5
44	88109.0
45	98265.0
46	108421.0
47	108421.0
48	108790.0
49	109159.0
50	98177.5
51	87196.0
52	79938.5
53	72681.0
54	72681.0
55	64525.5
56	56370.0
57	49128.0
58	41886.0
59	37005.0
60	32124.0
61	32124.0
62	28086.0
63	24048.0
64	20711.5
65	17375.0
66	14697.0
67	12019.0
68	12019.0
69	10253.5
70	8488.0
71	7055.0
72	5622.0
73	4512.5
74	3403.0
75	3403.0
76	2685.5
77	1968.0
78	1549.0
79	1130.0
80	889.5
81	649.0
82	649.0
83	486.0
84	323.0
85	259.0
86	195.0
87	151.0
88	107.0
89	107.0
90	86.5
91	66.0
92	41.5
93	17.0
94	11.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1249401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.480040628353045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.34328414283551	37.27988760461049
2	13.378813840409217	13.239685047652403
3	4.817263963778854	7.150752499358361
4	2.2169934683662755	4.387877075502265
5	1.139570168569383	2.8192989119836103
6	0.6603449933266469	1.96043382591192
7	0.43024058788066616	1.4901825237811301
8	0.29102393334612897	1.1519900836631654
9	0.22900167958307274	1.0197911168758371
>10	1.295161433212286	11.98318724939875
>50	0.12349741375411323	4.205436837351889
>100	0.06596535048587707	6.0160670365064375
>500	0.0057289973300028215	1.88977980708171
>1k	0.00261897020800129	2.5567466228069864
>5k	1.6368563800008064E-4	0.7956626590995894
>10k+	3.273712760001613E-4	2.0532210984154817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12998	1.0403385302236832	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12353	0.9887137916489581	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9824	0.7862967934234084	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4520	0.36177336179497216	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3549	0.2840561196925567	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2694	0.21562332669815376	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2235	0.17888572203800063	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1940	0.15527440749607213	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1937	0.15503429243293387	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1896	0.1517527199033777	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1717	0.13742585446946176	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1658	0.13270359156107606	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1580	0.12646059991948141	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1447	0.11581549878701873	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1442	0.11541530701512165	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1313	0.10509035930017663	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1296	0.10372970727572653	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1262	0.1010084032268263	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6007670875883723E-4	0.0
10	0.0	0.0	0.0	3.2015341751767445E-4	0.0
11	0.0	0.0	0.0	4.001917718970931E-4	0.0
12	0.0	0.0	0.0	4.802301262765117E-4	0.0
13	0.0	0.0	0.0	4.802301262765117E-4	0.0
14	0.0	0.0	0.0	5.602684806559304E-4	0.0
15	0.0	0.0	0.0	7.203451894147676E-4	0.0
16	0.0	0.0	0.0	0.0011205369613118607	0.0
17	0.0	0.0	0.0	0.002080997213864884	0.0
18	8.003835437941861E-5	0.0	0.0	0.0022410739226237215	0.0
19	8.003835437941861E-5	0.0	0.0	0.002961419112038489	0.0
20	8.003835437941861E-5	0.0	0.0	0.003761802655832675	0.0
21	8.003835437941861E-5	0.0	0.0	0.004722262908385699	0.0
22	8.003835437941861E-5	0.0	0.0	0.007123413539768257	0.0
23	8.003835437941861E-5	0.0	0.0	0.012405944928809885	0.0
24	8.003835437941861E-5	0.0	0.0	0.022890969352513724	0.0
25	8.003835437941861E-5	0.0	0.0	0.0300944212466614	0.0
26	8.003835437941861E-5	0.0	0.0	0.041539905922918265	0.0
27	8.003835437941861E-5	0.0	0.0	0.10509035930017664	0.0
28	8.003835437941861E-5	0.0	0.0	0.17096192495443815	0.0
29	8.003835437941861E-5	0.0	0.0	0.23963483301197935	0.0
30	8.003835437941861E-5	0.0	0.0	0.36857662191722274	0.0
31	8.003835437941861E-5	0.0	0.0	0.5688325845745281	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3375	0.0	25.214813	1
ATCTGCG	115	0.0	24.130432	36
TACGACG	40	0.0019312802	23.125002	5
TCACGTT	115	5.456968E-12	22.521738	24
GCCGGAA	135	0.0	21.925924	15
CCCGTAT	60	3.7266214E-5	21.583332	1
ATCTACG	45	0.0038259907	20.555557	1
CGCATTG	90	9.480027E-8	20.555557	14
CGCCGGA	150	0.0	19.733334	14
TAAACGT	100	2.875422E-7	18.499998	4
CAATGCG	75	2.0674472E-4	17.266666	19
CCGGTCC	75	2.0674472E-4	17.266666	9
TGGCGTA	65	0.0015800382	17.076923	24
ATCACGT	130	1.3933459E-8	17.076923	23
ACGCTAT	65	0.0015800382	17.076923	22
GTATCAA	5275	0.0	16.763979	2
TATACCG	190	1.8189894E-12	16.552631	5
CGTCGCA	90	4.4464534E-5	16.444445	11
ACGTTTA	135	2.2197128E-8	16.444443	26
CTGTGCG	125	1.6579361E-7	16.279999	9
>>END_MODULE