FastQCFastQC Report
Wed 25 May 2016
SRR2088262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550407
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT384581.5079161874947802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT371161.4552971349278763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT316461.2408215629897503No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG198380.7778366354860224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129940.5094873092804404No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA121350.47580641050624467No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT114830.4502418633574955No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT111110.4356559560885772No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA108430.42514782934645334No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA88670.34766999933736065No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT82670.3241443424520086No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT80750.316616132248696No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG79350.31112681230878053No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA68850.26995691275941447No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA65370.2563120317659103No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA64740.25384183779294833No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT58120.22788519636277663No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48190.188950234217519No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC47120.1847548254062979No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA45760.17942234317895145No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40070.1571121785660093No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG40000.1568377125690135No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38870.15240704718893885No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38470.15083867006324875No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36630.1436241352850741No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.12499965691750375No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC30840.1209218763907094No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA29600.11605990730106999No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.11523650931008265No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG29190.1144523207472376No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC29000.11370734161253479No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC27660.10845327824147283No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC27110.10629675969364889No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA27050.10606150312479537No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG25690.10072902089744891No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA25620.10045455490045314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90450.028.7368721
TGGACCG909.489668E-820.5555575
ATTGGAC9750.019.7333343
GTAATAC1700.019.5882343
GTATTGG9800.019.2551041
CGTCTGT9650.019.17098234
AGTATCA5750.018.9826091
TTGGACC17600.017.8693184
GTATCAA156400.017.4354212
GCGCATT852.7252248E-517.41176411
ATCGGTC752.0685112E-417.26666616
GGACCCT18250.016.8273966
TATTGGA12700.016.7519682
TGGACCC18700.016.422465
ATAACGC1251.6599188E-716.2799993
TACCCCG803.3845886E-416.18755
TAGGACG803.3845886E-416.18754
GCGTCTG11750.016.05957433
TGCGTCT11750.015.587234532
ATAACAC1353.9773659E-715.0740753
Produced by FastQC (version 0.11.5)