FastQCFastQC Report
Wed 25 May 2016
SRR2088261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714894
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT339351.249956720225541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT301431.110282758737542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270930.9979395143972472No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG171110.6302640176743548No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116880.4305140458522506No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT102780.37857831650149143No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT96370.35496781826472784No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA88540.326126913242285No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86320.31794979840833565No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA73130.2693659494624836No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT70500.2596786467537959No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG69950.2576527849706103No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT68620.2527538828403614No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56280.20730091119579622No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54860.20207050441011692No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46760.17223508542138294No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43620.16066925633192308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41420.15256580919918053No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38480.14173665712178818No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35040.12906581251422708No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33870.12475625199363216No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG32790.12077819612846762No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31960.11772098652838747No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31660.11661597101028623No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28610.10538164657625676No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.10457130186298248No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.10331895094246772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.029.9196011
GTATTGG9250.021.81
ATTGGAC10350.019.4830913
TAATACG701.2199739E-418.54
TTGGACC16500.018.54
ACCGTCC609.241235E-418.58
GTATCAA131000.017.7232822
TGGACCC17300.017.6445085
ACCGACC1401.873559E-917.1785728
TATTGGA12850.016.9883272
CGTTTAG1201.0427175E-716.95833226
CGAATTA1757.2759576E-1216.91428615
GGACCCT17800.016.7331456
TGTTACG904.449547E-516.44444516
CGAACGA700.002593713815.85714216
CGTCTGT7350.015.85714234
GACCCTC17650.015.8271957
GAACCGT1201.9377676E-615.4166666
GCGCTTA855.3674943E-415.23529418
TTTTTAC18300.014.8606551
Produced by FastQC (version 0.11.5)