##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088261_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2714894
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.047335549748905	31.0	31.0	33.0	27.0	34.0
2	30.869693255058944	31.0	31.0	34.0	27.0	34.0
3	30.8664673464231	31.0	31.0	34.0	27.0	34.0
4	35.03254086531555	35.0	35.0	37.0	32.0	37.0
5	34.939014930233	35.0	35.0	37.0	32.0	37.0
6	35.049287375492376	36.0	35.0	37.0	32.0	37.0
7	34.689217332242066	35.0	35.0	37.0	31.0	37.0
8	34.90070588391296	36.0	35.0	37.0	32.0	37.0
9	36.33968287527985	38.0	35.0	39.0	32.0	39.0
10	36.26606121638635	38.0	35.0	39.0	32.0	39.0
11	36.353850647575925	38.0	35.0	39.0	32.0	39.0
12	36.101928104743685	38.0	35.0	39.0	31.0	39.0
13	36.27220068260492	38.0	35.0	39.0	32.0	39.0
14	37.203626366259606	39.0	36.0	40.0	32.0	41.0
15	37.248544142054904	39.0	36.0	40.0	32.0	41.0
16	37.16089615285164	39.0	36.0	40.0	31.0	41.0
17	37.24493368801876	39.0	36.0	40.0	32.0	41.0
18	37.24291224629764	39.0	36.0	40.0	32.0	41.0
19	37.327019397442406	39.0	36.0	40.0	32.0	41.0
20	37.28576843147467	39.0	36.0	40.0	31.0	41.0
21	37.23004544560487	39.0	36.0	40.0	31.0	41.0
22	37.14462148430105	39.0	36.0	40.0	31.0	41.0
23	37.0321043841859	39.0	36.0	40.0	31.0	41.0
24	36.957753783388966	39.0	36.0	40.0	31.0	41.0
25	36.87793519746996	39.0	35.0	40.0	31.0	41.0
26	36.61872655064986	38.0	35.0	40.0	30.0	41.0
27	36.470085756570974	38.0	35.0	40.0	30.0	41.0
28	36.306632229471944	38.0	35.0	40.0	30.0	41.0
29	36.1443805909181	38.0	35.0	40.0	30.0	41.0
30	35.98009425045692	38.0	35.0	40.0	29.0	41.0
31	35.73208419923577	38.0	34.0	40.0	29.0	41.0
32	35.509213619389925	38.0	34.0	40.0	27.0	41.0
33	35.63436951866261	38.0	34.0	40.0	29.0	41.0
34	35.630556846786654	38.0	34.0	40.0	28.0	41.0
35	35.5374091953498	38.0	34.0	40.0	27.0	41.0
36	35.44874459186988	38.0	34.0	40.0	27.0	41.0
37	35.2918994259076	38.0	34.0	40.0	26.0	41.0
38	35.15440934342188	38.0	34.0	40.0	26.0	41.0
39	35.11524906681439	38.0	34.0	40.0	25.0	41.0
40	34.96191085176806	38.0	33.0	40.0	25.0	41.0
41	34.80281697922644	38.0	33.0	40.0	24.0	41.0
42	34.615411504095555	38.0	33.0	40.0	23.0	41.0
43	34.27077152920151	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	4.0
15	10.0
16	41.0
17	86.0
18	230.0
19	524.0
20	1089.0
21	2088.0
22	3775.0
23	6492.0
24	10663.0
25	16928.0
26	25824.0
27	37062.0
28	51788.0
29	70509.0
30	90392.0
31	111543.0
32	136913.0
33	166968.0
34	201883.0
35	241659.0
36	295535.0
37	372239.0
38	440702.0
39	429942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.640148013145264	24.944914976422652	13.31116426645018	22.1037727439819
2	17.95572865828279	22.950877640158325	36.0284416260819	23.06495207547698
3	19.793553634138203	26.59356129557913	32.04368200010755	21.569203070175117
4	13.021944871512478	15.991895079513233	35.53888291771244	35.44727713126184
5	11.190492151811451	40.574585969102294	34.826847751698594	13.408074127387662
6	31.5400527608076	36.17305132355075	15.496221951943612	16.79067396369803
7	24.417306900379902	35.33265018818415	21.717827657359734	18.532215254076217
8	27.550062728047575	33.98644661633198	20.20811862267919	18.25537203294125
9	25.164628895271786	13.68414383765996	23.59322316083059	37.55800410623766
10	16.150059634004126	27.856667700470073	33.96220994263496	22.031062722890837
11	31.447268291137703	21.765969500098347	25.190780929200184	21.59598127956377
12	20.05861002308009	27.37561761158999	32.069318360127504	20.496454005202413
13	30.772103809577832	20.934371655025945	28.982126005656205	19.31139852974002
14	20.69830350650891	22.644530504690056	28.718100964531214	27.939065024269826
15	23.105579812692504	28.89431410581776	27.058846496400964	20.941259585088773
16	21.807849588234383	28.469693476062048	27.368545512274146	22.353911423429423
17	19.279168910462065	27.29174693376611	28.233698995246225	25.195385160525603
18	20.398733799551657	26.555732931009462	32.383842610429724	20.661690659009153
19	19.717086560285598	25.118328745063344	33.74382204240755	21.420762652243514
20	24.088785786848398	23.95946213737995	32.52178538093936	19.429966694832284
21	23.365479462549917	24.884802132237944	31.249875685754212	20.499842719457924
22	21.780334701833663	24.539668952084316	32.19381677516691	21.486179570915105
23	20.739741588437706	26.408360694745358	33.23971396304975	19.61218375376718
24	19.5021610420149	25.62483102471036	31.640019831345167	23.23298810192958
25	20.72784425469282	26.511237639480584	32.709011843556326	20.05190626227028
26	22.002332319420205	25.45101208371303	31.350174260947206	21.19648133591956
27	20.8575362426673	26.86845232263212	30.911925106468246	21.362086328232337
28	20.45626827419413	26.35712480855606	31.238015185859926	21.94859173138988
29	19.4978146476437	26.27712168504553	32.218790125876	22.00627354143477
30	19.04033822314978	26.481475888193057	33.97749599063536	20.5006898980218
31	20.363446970673625	25.945801198868168	32.75380180589003	20.93695002456818
32	21.47008317820143	26.284414787465	30.81678327035973	21.42871876397384
33	22.427468623084362	26.05505039975778	30.441667335814955	21.075813641342904
34	20.663090345332083	28.70907667113338	30.37488756467103	20.2529454188635
35	19.468936908770658	29.53872232212381	30.652467462818066	20.339873306287465
36	20.379064523329458	26.094609955305803	32.60985511773203	20.9164704036327
37	20.513986918089618	26.37027449322147	31.736008846017562	21.37972974267135
38	20.79064596997157	25.981124861596804	32.25551347492757	20.972715693504057
39	21.6693911438163	25.49528637213829	32.19123840562468	20.64408407842074
40	20.108409389095854	26.133469667692367	31.438022994636256	22.320097948575523
41	20.977283090978872	25.72188822105025	31.23366879148873	22.067159896482146
42	18.630340632083612	27.358305701806408	31.61268911419746	22.39866455191252
43	19.356519996729155	26.988493841748518	31.348295734566435	22.306690426955896
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	244.0
1	343.5
2	443.0
3	1106.5
4	1770.0
5	1770.0
6	3115.0
7	4460.0
8	5030.0
9	5600.0
10	8706.5
11	11813.0
12	11813.0
13	21340.5
14	30868.0
15	50151.0
16	69434.0
17	67695.0
18	65956.0
19	65956.0
20	71602.0
21	77248.0
22	63938.5
23	50629.0
24	54889.5
25	59150.0
26	59150.0
27	65030.0
28	70910.0
29	74722.0
30	78534.0
31	83537.0
32	88540.0
33	88540.0
34	95574.5
35	102609.0
36	110231.0
37	117853.0
38	122635.0
39	127417.0
40	127417.0
41	133369.0
42	139321.0
43	158185.0
44	177049.0
45	221629.0
46	266209.0
47	266209.0
48	268054.5
49	269900.0
50	245335.0
51	220770.0
52	205549.5
53	190329.0
54	190329.0
55	165859.5
56	141390.0
57	118741.0
58	96092.0
59	84827.0
60	73562.0
61	73562.0
62	64309.5
63	55057.0
64	48195.0
65	41333.0
66	34990.5
67	28648.0
68	28648.0
69	24167.0
70	19686.0
71	16734.5
72	13783.0
73	10990.5
74	8198.0
75	8198.0
76	6426.5
77	4655.0
78	3510.0
79	2365.0
80	1893.5
81	1422.0
82	1422.0
83	1061.5
84	701.0
85	574.0
86	447.0
87	350.5
88	254.0
89	254.0
90	220.0
91	186.0
92	114.0
93	42.0
94	27.0
95	12.0
96	12.0
97	6.5
98	1.0
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2714894.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.030655479797225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43288937336622	31.598653635113855
2	13.995705147832691	12.044887328264261
3	5.162228985224434	6.664022909130477
4	2.4334508686679497	4.188519438266553
5	1.3433796709779295	2.890325390020732
6	0.791730148837802	2.0441200360564826
7	0.5288863534058971	1.5930828522962803
8	0.35914306590338074	1.236332922947719
9	0.2694812817579692	1.0436360574223207
>10	1.4473405371493115	11.591445996784786
>50	0.1419499532014283	4.232906374200471
>100	0.07997700213130317	6.516381981150947
>500	0.006226925194630072	1.8219393294100792
>1k	0.0063134102667768395	5.213350794351024
>5k	7.783656493286514E-4	2.4732034645626357
>10k+	5.189104328857676E-4	4.84719149002143
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33935	1.249956720225541	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30143	1.110282758737542	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27093	0.9979395143972472	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17111	0.6302640176743548	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11688	0.4305140458522506	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10278	0.37857831650149143	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9637	0.35496781826472784	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8854	0.326126913242285	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8632	0.31794979840833565	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7313	0.2693659494624836	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7050	0.2596786467537959	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6995	0.2576527849706103	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6862	0.2527538828403614	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5628	0.20730091119579622	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5486	0.20207050441011692	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4676	0.17223508542138294	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4362	0.16066925633192308	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4142	0.15256580919918053	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3848	0.14173665712178818	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3504	0.12906581251422708	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3387	0.12475625199363216	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3279	0.12077819612846762	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3196	0.11772098652838747	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3166	0.11661597101028623	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2861	0.10538164657625676	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2839	0.10457130186298248	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2805	0.10331895094246772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.6833850603375304E-5	0.0	0.0	7.366770120675061E-5	0.0
3	3.6833850603375304E-5	0.0	0.0	7.366770120675061E-5	0.0
4	3.6833850603375304E-5	0.0	0.0	1.1050155181012592E-4	0.0
5	3.6833850603375304E-5	3.6833850603375304E-5	0.0	1.1050155181012592E-4	0.0
6	3.6833850603375304E-5	3.6833850603375304E-5	0.0	1.1050155181012592E-4	0.0
7	3.6833850603375304E-5	3.6833850603375304E-5	0.0	1.4733540241350121E-4	0.0
8	3.6833850603375304E-5	3.6833850603375304E-5	0.0	2.2100310362025184E-4	0.0
9	3.6833850603375304E-5	3.6833850603375304E-5	0.0	2.5783695422362715E-4	0.0
10	3.6833850603375304E-5	3.6833850603375304E-5	0.0	5.156739084472543E-4	0.0
11	3.6833850603375304E-5	7.366770120675061E-5	0.0	6.630093108607555E-4	0.0
12	3.6833850603375304E-5	7.366770120675061E-5	0.0	9.208462650843826E-4	0.0
13	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.0010313478168945086	0.0
14	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.0012891847711181358	0.0
15	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.0016575232771518888	0.0
16	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.0024678679904261455	0.0
17	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.004199058968784785	0.0
18	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.00478840057843879	0.0
19	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.0055619114411096715	0.0
20	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.007072099315848059	0.0
21	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.009981973513514708	0.0
22	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.016759402024535765	0.0
23	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.027330717147704478	0.0
24	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.044605793080687496	0.0
25	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.05197256320136256	0.0
26	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.06184403516306714	0.0
27	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.13002349262991483	0.0
28	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.19470373428944188	0.0
29	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.2697342879685174	0.0
30	3.6833850603375304E-5	7.366770120675061E-5	0.0	0.4137546438277148	0.0
31	1.8416925301687653E-4	7.366770120675061E-5	0.0	0.6100422336931018	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7525	0.0	29.919601	1
GTATTGG	925	0.0	21.8	1
ATTGGAC	1035	0.0	19.483091	3
TAATACG	70	1.2199739E-4	18.5	4
TTGGACC	1650	0.0	18.5	4
ACCGTCC	60	9.241235E-4	18.5	8
GTATCAA	13100	0.0	17.723282	2
TGGACCC	1730	0.0	17.644508	5
ACCGACC	140	1.873559E-9	17.178572	8
TATTGGA	1285	0.0	16.988327	2
CGTTTAG	120	1.0427175E-7	16.958332	26
CGAATTA	175	7.2759576E-12	16.914286	15
GGACCCT	1780	0.0	16.733145	6
TGTTACG	90	4.449547E-5	16.444445	16
CGAACGA	70	0.0025937138	15.857142	16
CGTCTGT	735	0.0	15.857142	34
GACCCTC	1765	0.0	15.827195	7
GAACCGT	120	1.9377676E-6	15.416666	6
GCGCTTA	85	5.3674943E-4	15.235294	18
TTTTTAC	1830	0.0	14.860655	1
>>END_MODULE