FastQCFastQC Report
Wed 25 May 2016
SRR2088260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553013
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300321.1763355689924024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT275841.0804488657127873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT252760.9900458791239998No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG175660.6880497670791336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105970.41507818409071945No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96760.37900316214606034No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94040.3683490840038809No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT93680.3669389854262395No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT92070.3606327112317877No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA74520.29189040557176954No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT69660.2728540747736106No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT64170.2513500714645793No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG63790.24986163407706893No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA55170.21609760702354436No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52570.20591356174057868No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50330.1971396150352544No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43600.17077860551434715No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA37230.1458276945710813No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC37050.14512264528226063No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35820.14030480847531918No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35670.13971726740130191No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG33050.12945488330846727No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30310.11872246635641887No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29510.11558891396166021No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27910.10932180917214288No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC25980.10176211401978759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.030.5427071
GTATTGG8700.023.1781621
ATTGGAC9050.022.0773493
CGAACGA656.9063535E-519.92307716
TTGGACC16250.018.7846154
GGACCCT16450.018.2188456
TATTGGA11400.018.1754382
GTATCAA118450.018.0548742
ACGTTTA1502.5283953E-1017.26666626
TGGACCC17200.017.2093035
TATACGG1201.0426447E-716.9583322
CGTCTGT6450.016.92248234
TACCCCG1251.6599188E-716.2799995
CAATGCG1251.6599188E-716.27999919
AGTGCGC1504.6784407E-916.0333338
GACCCTC18300.015.9726787
TAGTGCG957.0650756E-515.5789487
GTATACG1701.4879333E-915.2352941
ACGAACG855.367337E-415.23529415
ACCCTCG19800.014.6691928
Produced by FastQC (version 0.11.5)