##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088260_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2553013
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.026571740919454	31.0	30.0	33.0	27.0	34.0
2	30.84607638112301	31.0	31.0	34.0	27.0	34.0
3	30.858951756219025	31.0	31.0	34.0	27.0	34.0
4	35.02326740991918	35.0	35.0	37.0	32.0	37.0
5	34.924787300338856	35.0	35.0	37.0	32.0	37.0
6	35.02387923602426	36.0	35.0	37.0	32.0	37.0
7	34.66599269177243	35.0	35.0	37.0	31.0	37.0
8	34.86807548571041	35.0	35.0	37.0	32.0	37.0
9	36.30372896651917	38.0	35.0	39.0	32.0	39.0
10	36.22573132216718	38.0	35.0	39.0	32.0	39.0
11	36.31310455528428	38.0	35.0	39.0	32.0	39.0
12	36.05715129535181	38.0	35.0	39.0	30.0	39.0
13	36.2236858958415	38.0	35.0	39.0	32.0	39.0
14	37.137148929519746	39.0	36.0	40.0	31.0	41.0
15	37.1754703168374	39.0	36.0	40.0	32.0	41.0
16	37.09540139435248	39.0	36.0	40.0	31.0	41.0
17	37.18233514674622	39.0	36.0	40.0	31.0	41.0
18	37.184272073820225	39.0	36.0	40.0	31.0	41.0
19	37.26335745254725	39.0	36.0	40.0	31.0	41.0
20	37.22418883100086	39.0	36.0	40.0	31.0	41.0
21	37.16148566419364	39.0	36.0	40.0	31.0	41.0
22	37.07808812567738	39.0	36.0	40.0	31.0	41.0
23	36.95382671376918	39.0	36.0	40.0	31.0	41.0
24	36.890501145117554	39.0	35.0	40.0	31.0	41.0
25	36.804212121129034	39.0	35.0	40.0	30.0	41.0
26	36.552950180825555	38.0	35.0	40.0	30.0	41.0
27	36.40454357263359	38.0	35.0	40.0	30.0	41.0
28	36.254275634319136	38.0	35.0	40.0	30.0	41.0
29	36.08763370965992	38.0	35.0	40.0	30.0	41.0
30	35.92608694119458	38.0	34.0	40.0	29.0	41.0
31	35.680486938374386	38.0	34.0	40.0	29.0	41.0
32	35.45482298758369	38.0	34.0	40.0	27.0	41.0
33	35.593306418729554	38.0	34.0	40.0	28.0	41.0
34	35.59827584113359	38.0	34.0	40.0	28.0	41.0
35	35.51494332382953	38.0	34.0	40.0	27.0	41.0
36	35.43311177812256	38.0	34.0	40.0	27.0	41.0
37	35.28285245707718	38.0	34.0	40.0	26.0	41.0
38	35.16262941081773	38.0	34.0	40.0	26.0	41.0
39	35.135515957028026	38.0	34.0	40.0	25.0	41.0
40	34.99215946021427	38.0	33.0	40.0	25.0	41.0
41	34.83809130623307	38.0	33.0	40.0	24.0	41.0
42	34.649307308658436	38.0	33.0	40.0	24.0	41.0
43	34.31695569117744	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	2.0
15	9.0
16	23.0
17	76.0
18	204.0
19	450.0
20	917.0
21	1813.0
22	3344.0
23	5955.0
24	9839.0
25	16146.0
26	24045.0
27	35283.0
28	49379.0
29	66831.0
30	85871.0
31	107199.0
32	130671.0
33	159031.0
34	193034.0
35	229904.0
36	280652.0
37	351054.0
38	412475.0
39	388802.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.666268836077215	24.757962454558594	13.306747752557468	22.269020956806724
2	17.93093102150283	22.863064152043094	36.12394453142229	23.08206029503179
3	19.615685466544825	26.73899427852502	31.977628002677623	21.667692252252536
4	12.949718626579653	15.867095075504903	35.737812537578144	35.4453737603373
5	11.273855636457785	40.353809400892196	34.972912398017556	13.399422564632458
6	31.54680371780324	36.34501665287251	15.540853101805594	16.56732652751866
7	24.60234240875389	34.94733477659534	21.797695507230085	18.652627307420683
8	27.65708596078438	33.66195158426534	20.266915992985542	18.414046461964745
9	25.273353484686524	13.603064300886835	23.39753068237412	37.726051532052516
10	16.123850524850443	27.557713180465594	34.13253281514822	22.18590347953575
11	31.569051939806027	21.80619526810087	25.164540877778528	21.460211914314577
12	20.102404492260714	27.253288565314786	31.935990925232264	20.708316017192235
13	30.912141849649803	20.832248014404943	28.74470282760017	19.51090730834508
14	20.933735942590186	22.30768115947706	28.28783088844436	28.470752009488397
15	22.990795581534446	28.855669751779562	26.833235866797388	21.3202987998886
16	21.92452604040794	28.412624612565622	27.038209362819536	22.624639984206894
17	19.51396252192997	27.085878528624807	27.885639438577087	25.51451951086814
18	20.614583631184015	26.477342653562673	32.07124288047103	20.836830834782276
19	19.979412560766434	24.891804311219722	33.25012446078418	21.87865866722966
20	24.315426517608802	23.843043494098932	32.04930801370772	19.79222197458454
21	23.695766531545274	24.774139418796537	30.831922908343984	20.698171141314205
22	22.069805363309943	24.22122409874137	31.87190977875945	21.83706075918924
23	21.057080398728875	26.321957624187576	32.86085891454528	19.760103062538263
24	19.797157319606285	25.371472844047403	31.236973724771477	23.594396111574834
25	21.103065280121957	26.24001522906464	32.373591517160314	20.283327973653094
26	22.474856179737433	25.332969318996806	30.896160732436538	21.296013768829223
27	20.997033700964312	26.822346772225604	30.587231635718265	21.59338789109182
28	20.770947895682472	26.149103040211703	30.795534531159852	22.284414532945974
29	19.957203508168583	26.089056342447144	31.7271396581216	22.226600491262676
30	19.33632927055209	26.413496523519463	33.40257178478919	20.847602421139257
31	20.762918167670904	25.80413809095371	32.47617618868372	20.956767552691662
32	21.79773467663502	25.9899577479629	30.38096554933328	21.831342026068807
33	22.65397786850282	25.969628826801898	29.95425405197702	21.42213925271826
34	20.90522061579788	28.636908625220475	30.114926951018266	20.342943807963376
35	19.74913562915661	29.594287220629116	30.19820110590898	20.4583760443053
36	20.74697621986257	26.00116019777416	32.25259722531769	20.999266357045578
37	20.8490125197169	26.380985917423843	31.27786658352308	21.492134979336182
38	21.023864743344433	25.997282426685647	31.883151397975645	21.095701431994275
39	21.9174363781148	25.466850345062873	31.87578754984796	20.739925726974363
40	20.34028028842783	26.204175223549587	30.900978569243478	22.554565918779105
41	21.32464660383633	25.778168775482147	30.755581738126676	22.14160288255485
42	18.935900443906867	27.459711329319514	31.231803363320125	22.372584863453497
43	19.690342352349948	27.119760063893132	30.918878987298537	22.27101859645838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	154.0
1	265.0
2	376.0
3	937.0
4	1498.0
5	1498.0
6	2683.5
7	3869.0
8	4362.0
9	4855.0
10	7406.5
11	9958.0
12	9958.0
13	18531.5
14	27105.0
15	44129.0
16	61153.0
17	59504.5
18	57856.0
19	57856.0
20	61972.5
21	66089.0
22	53742.0
23	41395.0
24	45509.5
25	49624.0
26	49624.0
27	55014.5
28	60405.0
29	63924.0
30	67443.0
31	73247.5
32	79052.0
33	79052.0
34	87037.5
35	95023.0
36	104440.5
37	113858.0
38	119130.0
39	124402.0
40	124402.0
41	132337.0
42	140272.0
43	157931.0
44	175590.0
45	216421.0
46	257252.0
47	257252.0
48	260267.5
49	263283.0
50	238940.5
51	214598.0
52	200059.5
53	185521.0
54	185521.0
55	160659.0
56	135797.0
57	111755.5
58	87714.0
59	77461.0
60	67208.0
61	67208.0
62	58519.5
63	49831.0
64	43646.5
65	37462.0
66	32005.0
67	26548.0
68	26548.0
69	22450.0
70	18352.0
71	15382.0
72	12412.0
73	9940.5
74	7469.0
75	7469.0
76	5864.0
77	4259.0
78	3324.5
79	2390.0
80	1877.0
81	1364.0
82	1364.0
83	1010.5
84	657.0
85	538.0
86	419.0
87	341.0
88	263.0
89	263.0
90	225.0
91	187.0
92	110.0
93	33.0
94	21.0
95	9.0
96	9.0
97	8.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2553013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.404736605935234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5625529022692	32.363679693891065
2	13.630331228441179	11.832418736442861
3	4.89047424272851	6.368092391512247
4	2.2661632870576605	3.9344888232311264
5	1.218709037837062	2.6448872393295217
6	0.6903366591634722	1.7978328516247082
7	0.45664376223390307	1.3874351555754114
8	0.3257086383299697	1.1309838125592127
9	0.247374482203364	0.9663501818759965
>10	1.4614641570941207	12.256258138226864
>50	0.15407260433555378	4.673633663634208
>100	0.08373067538343096	6.6825874231808635
>500	0.0050302041231117915	1.5257624762593025
>1k	0.0059447866909502515	4.824402595025157
>5k	0.0010060408246223502	3.2026125545647126
>10k+	4.572912839192501E-4	4.408574263066887
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30032	1.1763355689924024	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27584	1.0804488657127873	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25276	0.9900458791239998	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17566	0.6880497670791336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10597	0.41507818409071945	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9676	0.37900316214606034	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9404	0.3683490840038809	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9368	0.3669389854262395	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9207	0.3606327112317877	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7452	0.29189040557176954	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6966	0.2728540747736106	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6417	0.2513500714645793	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6379	0.24986163407706893	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5517	0.21609760702354436	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5257	0.20591356174057868	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5033	0.1971396150352544	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4360	0.17077860551434715	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3723	0.1458276945710813	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3705	0.14512264528226063	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3582	0.14030480847531918	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3567	0.13971726740130191	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3305	0.12945488330846727	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3031	0.11872246635641887	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2951	0.11558891396166021	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2791	0.10932180917214288	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2598	0.10176211401978759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.833880986896659E-5	0.0
5	0.0	0.0	0.0	7.833880986896659E-5	0.0
6	3.9169404934483294E-5	0.0	0.0	7.833880986896659E-5	0.0
7	3.9169404934483294E-5	0.0	0.0	7.833880986896659E-5	0.0
8	3.9169404934483294E-5	0.0	0.0	7.833880986896659E-5	0.0
9	3.9169404934483294E-5	0.0	0.0	1.1750821480344989E-4	0.0
10	3.9169404934483294E-5	0.0	0.0	1.9584702467241648E-4	0.0
11	3.9169404934483294E-5	0.0	0.0	2.741858345413831E-4	0.0
12	3.9169404934483294E-5	0.0	0.0	6.267104789517327E-4	0.0
13	3.9169404934483294E-5	0.0	0.0	7.050492888206993E-4	0.0
14	3.9169404934483294E-5	0.0	0.0	9.792351233620824E-4	0.0
15	3.9169404934483294E-5	0.0	0.0	0.0012534209579034654	0.0
16	3.9169404934483294E-5	0.0	0.0	0.002154317271396581	0.0
17	3.9169404934483294E-5	0.0	0.0	0.0032510606095621135	0.0
18	3.9169404934483294E-5	0.0	0.0	0.00344690763423453	0.0
19	3.9169404934483294E-5	0.0	0.0	0.0041911263279897125	0.0
20	3.9169404934483294E-5	0.0	0.0	0.005248700261220761	0.0
21	3.9169404934483294E-5	0.0	0.0	0.007559695152355276	0.0
22	3.9169404934483294E-5	0.0	0.0	0.012651717793838104	0.0
23	3.9169404934483294E-5	0.0	0.0	0.02318828772121411	0.0
24	3.9169404934483294E-5	0.0	0.0	0.03920857433941778	0.0
25	3.9169404934483294E-5	0.0	0.0	0.04508398507959027	0.0
26	3.9169404934483294E-5	0.0	0.0	0.05307454368622486	0.0
27	3.9169404934483294E-5	0.0	0.0	0.10595324034777731	0.0
28	3.9169404934483294E-5	0.0	0.0	0.16086874606592289	0.0
29	3.9169404934483294E-5	0.0	0.0	0.2276525814792169	0.0
30	3.9169404934483294E-5	0.0	0.0	0.3473934523639323	0.0
31	3.9169404934483294E-5	0.0	0.0	0.511082395585138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6790	0.0	30.542707	1
GTATTGG	870	0.0	23.178162	1
ATTGGAC	905	0.0	22.077349	3
CGAACGA	65	6.9063535E-5	19.923077	16
TTGGACC	1625	0.0	18.784615	4
GGACCCT	1645	0.0	18.218845	6
TATTGGA	1140	0.0	18.175438	2
GTATCAA	11845	0.0	18.054874	2
ACGTTTA	150	2.5283953E-10	17.266666	26
TGGACCC	1720	0.0	17.209303	5
TATACGG	120	1.0426447E-7	16.958332	2
CGTCTGT	645	0.0	16.922482	34
TACCCCG	125	1.6599188E-7	16.279999	5
CAATGCG	125	1.6599188E-7	16.279999	19
AGTGCGC	150	4.6784407E-9	16.033333	8
GACCCTC	1830	0.0	15.972678	7
TAGTGCG	95	7.0650756E-5	15.578948	7
GTATACG	170	1.4879333E-9	15.235294	1
ACGAACG	85	5.367337E-4	15.235294	15
ACCCTCG	1980	0.0	14.669192	8
>>END_MODULE