FastQCFastQC Report
Wed 25 May 2016
SRR2088259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551245
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235261.5165882887616076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT216461.3953953115078532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT171591.1061437748389196No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG108170.6973108696563083No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78330.504949250440775No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA70610.455182772547212No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70420.45395794990475397No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT68690.4428056174234244No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT65530.42243488294885717No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA51200.3300574699676711No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG49530.31929192358395997No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT44420.2863506409367959No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43160.27822813288681025No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37350.24077434576743198No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34910.22504504446428514No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32660.21054056580359645No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.18701107819847929No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT27770.17901749884769977No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25810.16638248632549985No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25420.16386837669098048No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC24860.16025837311320906No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC23910.1541342599009183No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG22920.14775228929021528No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT21600.13924299514261126No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20090.1295088783525491No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC19690.12693030436842664No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.12673691131961745No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA17350.11184564656131044No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.11165225351250124No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA17250.11120100306527983No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC16480.10623724814584415No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA16370.10552814030021047No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG15630.10075777842958399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50950.029.6290471
ACGAACG450.003826402820.55555515
GTATTGG6450.018.6434121
ATTGGAC6850.018.6350363
CGAACGA500.007035964618.516
GACGGTT609.238799E-418.532
TTCGCGG609.238799E-418.532
GCGTCCG500.007035964618.511
GTGCGAA609.238799E-418.513
ATCGACC500.007035964618.57
ACGGTTT609.238799E-418.533
GTATCAA87300.017.6523482
CCGATAA650.001580270217.0769239
ACGGTGT1201.04186256E-716.95833429
AAGACCG1005.8804562E-616.6500025
TATACTG1452.9813236E-916.5862065
ACCGACC1801.0913936E-1116.4444458
CTGACCG904.4475702E-516.4444459
CGGTTTA803.383519E-416.187515
CCCGTAT803.383519E-416.18751
Produced by FastQC (version 0.11.5)