##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088259_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1551245
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.93678625877924	31.0	30.0	31.0	27.0	34.0
2	30.632306308803575	31.0	30.0	33.0	27.0	34.0
3	30.619123993953245	31.0	30.0	34.0	27.0	34.0
4	34.747756157151194	35.0	35.0	37.0	32.0	37.0
5	34.60452152948116	35.0	35.0	37.0	32.0	37.0
6	34.726805243530194	35.0	35.0	37.0	31.0	37.0
7	34.37528694693617	35.0	35.0	37.0	30.0	37.0
8	34.588369342044615	35.0	35.0	37.0	30.0	37.0
9	35.93534419127862	37.0	35.0	39.0	30.0	39.0
10	35.827445052200005	37.0	35.0	39.0	30.0	39.0
11	35.95068799577114	37.0	35.0	39.0	30.0	39.0
12	35.677826519988784	37.0	35.0	39.0	30.0	39.0
13	35.86087561926066	37.0	35.0	39.0	30.0	39.0
14	36.67291691512301	38.0	36.0	40.0	31.0	41.0
15	36.723424733037014	38.0	36.0	40.0	31.0	41.0
16	36.60227236832351	38.0	35.0	40.0	30.0	41.0
17	36.73042427211691	38.0	36.0	40.0	31.0	41.0
18	36.73038301493317	38.0	36.0	40.0	31.0	41.0
19	36.81973382670049	39.0	36.0	40.0	31.0	41.0
20	36.79391778861495	39.0	36.0	40.0	31.0	41.0
21	36.72258347327469	38.0	35.0	40.0	30.0	41.0
22	36.63088744846881	38.0	35.0	40.0	30.0	41.0
23	36.49207636446854	38.0	35.0	40.0	30.0	41.0
24	36.41867145421903	38.0	35.0	40.0	30.0	41.0
25	36.328432968357674	38.0	35.0	40.0	30.0	41.0
26	36.040377245373875	38.0	34.0	40.0	29.0	41.0
27	35.86672511434364	38.0	34.0	40.0	29.0	41.0
28	35.67937108580527	38.0	34.0	40.0	27.0	41.0
29	35.49187716962827	38.0	34.0	40.0	27.0	41.0
30	35.31057892209161	38.0	34.0	40.0	27.0	41.0
31	35.01758265135423	37.0	33.0	40.0	26.0	41.0
32	34.80831203323782	37.0	33.0	40.0	26.0	41.0
33	34.93269148329245	37.0	33.0	40.0	26.0	41.0
34	34.90960164255163	37.0	33.0	40.0	26.0	41.0
35	34.792309403092354	37.0	33.0	40.0	25.0	41.0
36	34.69271842939059	37.0	33.0	40.0	25.0	41.0
37	34.516428417174595	37.0	33.0	40.0	24.0	41.0
38	34.371856798893795	37.0	33.0	40.0	24.0	41.0
39	34.303590986594635	37.0	33.0	40.0	24.0	41.0
40	34.11461567966376	37.0	33.0	40.0	23.0	41.0
41	33.92944763722043	37.0	33.0	40.0	22.0	41.0
42	33.69154453358431	37.0	32.0	40.0	21.0	41.0
43	33.32774964625188	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	6.0
15	6.0
16	28.0
17	80.0
18	178.0
19	448.0
20	869.0
21	1676.0
22	3229.0
23	5353.0
24	8753.0
25	13282.0
26	19702.0
27	27646.0
28	37327.0
29	49203.0
30	61967.0
31	74089.0
32	89306.0
33	105961.0
34	124716.0
35	145764.0
36	174487.0
37	211725.0
38	237081.0
39	158362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.00602419347041	25.738229615566848	14.170553329744816	21.085192861217923
2	18.90584659418725	24.05158437255237	34.65977327888245	22.382795754377934
3	21.084161431624278	26.31234911313171	32.44013679334986	20.16335266189416
4	13.826055845466062	16.56972302892193	34.71914494486686	34.885076180745145
5	10.981953205328624	41.3015996828354	34.4186121470174	13.29783496481858
6	31.896895719244867	36.2025340935829	14.647589516807468	17.25298067036477
7	23.937289080706144	36.03041428014272	21.117811822117076	18.91448481703406
8	28.08466747676866	33.66180068267746	19.9852376639409	18.26829417661298
9	24.867638574177516	13.451904760369896	23.93361461277877	37.74684205267382
10	16.655976328690826	28.208890278453758	33.496449625945615	21.638683766909807
11	31.430044899419496	21.514654358273518	24.966204564720595	22.08909617758639
12	19.983819448249633	27.806117022133837	31.69918355901228	20.510879970604257
13	31.426692753240136	20.4295904257548	29.413793436884568	18.729923384120497
14	20.776279697920057	22.591982568839867	28.802413545249138	27.829324187990935
15	23.30605416939297	28.889633810262076	27.073802010643067	20.730510009701884
16	21.704566332204134	28.0960776666484	27.64882400910237	22.5505319920451
17	19.149779693085232	26.996896041566615	28.472227146582263	25.381097118765894
18	20.3033047648824	25.669832940638003	33.214547025131424	20.81231526934817
19	19.54952312497381	24.136741778378013	34.767557671418764	21.546177425229413
20	24.137644279272454	23.21799586783519	33.371968966862106	19.27239088603025
21	23.86328400736183	23.91382405745063	31.91159359095436	20.31129834423318
22	21.8217625197825	23.681623470180405	32.93328906781327	21.563324942223826
23	20.89740821082421	26.070285480372217	33.93139059271746	19.10091571608611
24	19.416146385645078	24.829733536610917	32.34672795077502	23.407392126968983
25	20.627882765133812	25.908222105470124	33.504120883548374	19.95977424584769
26	22.072270982339994	24.863448391453318	32.094543415127845	20.969737211078844
27	20.869495147446084	26.37868292887326	31.64129457306873	21.110527350611928
28	20.671654058514292	25.83389471037779	31.89083607038218	21.603615160725738
29	19.66368948812083	25.670799905882046	32.67987970952364	21.985630896473477
30	18.90984338386264	26.006723631663597	34.71901601616766	20.364416968306102
31	20.45872831177538	25.355633700672687	33.457642087484565	20.727995900067363
32	21.400939245573717	25.8277705971655	31.237232029756747	21.53405812750404
33	22.447195639631392	25.789478773501283	30.658793420768482	21.104532166098842
34	20.811541697152933	28.790938891019795	30.74072760911397	19.656791802713304
35	19.371601520069365	29.961547015461775	30.85418486441536	19.812666600053504
36	20.595972912080295	25.94316178295498	33.10953459962804	20.351330705336682
37	20.461758136206722	26.547901846581297	32.148564540095215	20.841775477116766
38	20.960518809085606	25.929366412139927	32.56751834816551	20.54259643060896
39	21.980473748505233	25.63669826494203	32.20703370518519	20.175794281367544
40	20.125447624327556	26.64801498151485	31.237425422805554	21.98911197135204
41	21.29173663734613	26.148158414692713	31.021792173383318	21.53831277457784
42	18.74958501074943	28.006020970252926	31.26901295411105	21.97538106488659
43	19.53630793330518	27.67441635589478	30.83987377880348	21.949401931996558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	87.0
1	137.5
2	188.0
3	491.0
4	794.0
5	794.0
6	1562.5
7	2331.0
8	2685.5
9	3040.0
10	4835.5
11	6631.0
12	6631.0
13	12982.5
14	19334.0
15	32456.0
16	45578.0
17	42915.0
18	40252.0
19	40252.0
20	44310.0
21	48368.0
22	38416.5
23	28465.0
24	30165.5
25	31866.0
26	31866.0
27	34330.0
28	36794.0
29	38241.5
30	39689.0
31	42443.0
32	45197.0
33	45197.0
34	49432.5
35	53668.0
36	58951.5
37	64235.0
38	67347.5
39	70460.0
40	70460.0
41	74290.0
42	78120.0
43	89544.5
44	100969.0
45	128149.5
46	155330.0
47	155330.0
48	159690.0
49	164050.0
50	149183.0
51	134316.0
52	125741.0
53	117166.0
54	117166.0
55	99550.0
56	81934.0
57	67102.0
58	52270.0
59	45582.5
60	38895.0
61	38895.0
62	34071.5
63	29248.0
64	25277.5
65	21307.0
66	17927.0
67	14547.0
68	14547.0
69	12326.5
70	10106.0
71	8493.5
72	6881.0
73	5486.5
74	4092.0
75	4092.0
76	3124.0
77	2156.0
78	1680.5
79	1205.0
80	975.0
81	745.0
82	745.0
83	561.5
84	378.0
85	301.0
86	224.0
87	191.5
88	159.0
89	159.0
90	141.0
91	123.0
92	77.5
93	32.0
94	22.0
95	12.0
96	12.0
97	7.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1551245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.917903150859054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21783381323347	35.1338108389281
2	12.135550337166267	10.902069494544186
3	3.8703658979675497	5.2154616168988195
4	1.7446273764623654	3.134600141210954
5	0.9048268908818071	2.032146332646096
6	0.5650185524911269	1.522766917154101
7	0.4026542810947888	1.2660470201046148
8	0.26765582551206074	0.961803075849118
9	0.2044054269979556	0.8263316856063744
>10	1.4455265179843866	12.566472253837556
>50	0.15054603412190587	4.644059678809953
>100	0.07560656064384272	6.222396195813378
>500	0.006385182636562026	1.969009073693185
>1k	0.007546124934118758	6.196443591033464
>5k	8.707067231675489E-4	2.638512572746474
>10k+	5.80471148778366E-4	4.76806951112355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23526	1.5165882887616076	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21646	1.3953953115078532	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17159	1.1061437748389196	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10817	0.6973108696563083	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7833	0.504949250440775	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7061	0.455182772547212	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7042	0.45395794990475397	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6869	0.4428056174234244	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6553	0.42243488294885717	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5120	0.3300574699676711	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4953	0.31929192358395997	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4442	0.2863506409367959	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4316	0.27822813288681025	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3735	0.24077434576743198	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3491	0.22504504446428514	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3266	0.21054056580359645	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2901	0.18701107819847929	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2777	0.17901749884769977	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2581	0.16638248632549985	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2542	0.16386837669098048	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2486	0.16025837311320906	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2391	0.1541342599009183	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2292	0.14775228929021528	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2160	0.13924299514261126	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2009	0.1295088783525491	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1969	0.12693030436842664	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1966	0.12673691131961745	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1735	0.11184564656131044	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1732	0.11165225351250124	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1725	0.11120100306527983	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1648	0.10623724814584415	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1637	0.10552814030021047	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1563	0.10075777842958399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0
7	0.0	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0
8	0.0	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0
9	0.0	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0
10	0.0	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0
11	0.0	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0
12	0.0	0.0	6.446434960306077E-5	2.578573984122431E-4	0.0
13	0.0	0.0	6.446434960306077E-5	3.223217480153038E-4	0.0
14	0.0	0.0	6.446434960306077E-5	4.5125044722142536E-4	0.0
15	0.0	0.0	6.446434960306077E-5	5.80179146427547E-4	0.0
16	0.0	0.0	6.446434960306077E-5	0.0012248226424581546	0.0
17	0.0	0.0	6.446434960306077E-5	0.0017405374392826408	0.0
18	0.0	0.0	6.446434960306077E-5	0.0021273235369010054	0.0
19	0.0	0.0	6.446434960306077E-5	0.0027075026833285522	0.0
20	0.0	0.0	6.446434960306077E-5	0.003867860976183646	0.0
21	0.0	0.0	6.446434960306077E-5	0.006059648862687712	0.0
22	0.0	6.446434960306077E-5	6.446434960306077E-5	0.010314295936489722	0.0
23	0.0	6.446434960306077E-5	6.446434960306077E-5	0.01953269792972741	0.0
24	0.0	6.446434960306077E-5	6.446434960306077E-5	0.03113628085827835	0.0
25	0.0	6.446434960306077E-5	6.446434960306077E-5	0.036615750574538516	0.0
26	0.0	6.446434960306077E-5	6.446434960306077E-5	0.04112825504675277	0.0
27	0.0	6.446434960306077E-5	6.446434960306077E-5	0.0757456107835964	0.0
28	0.0	6.446434960306077E-5	6.446434960306077E-5	0.1176474380255859	0.0
29	0.0	6.446434960306077E-5	6.446434960306077E-5	0.16734945156954575	0.0
30	0.0	6.446434960306077E-5	6.446434960306077E-5	0.2698477674384124	0.0
31	0.0	6.446434960306077E-5	6.446434960306077E-5	0.3969714648556482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5095	0.0	29.629047	1
ACGAACG	45	0.0038264028	20.555555	15
GTATTGG	645	0.0	18.643412	1
ATTGGAC	685	0.0	18.635036	3
CGAACGA	50	0.0070359646	18.5	16
GACGGTT	60	9.238799E-4	18.5	32
TTCGCGG	60	9.238799E-4	18.5	32
GCGTCCG	50	0.0070359646	18.5	11
GTGCGAA	60	9.238799E-4	18.5	13
ATCGACC	50	0.0070359646	18.5	7
ACGGTTT	60	9.238799E-4	18.5	33
GTATCAA	8730	0.0	17.652348	2
CCGATAA	65	0.0015802702	17.076923	9
ACGGTGT	120	1.04186256E-7	16.958334	29
AAGACCG	100	5.8804562E-6	16.650002	5
TATACTG	145	2.9813236E-9	16.586206	5
ACCGACC	180	1.0913936E-11	16.444445	8
CTGACCG	90	4.4475702E-5	16.444445	9
CGGTTTA	80	3.383519E-4	16.1875	15
CCCGTAT	80	3.383519E-4	16.1875	1
>>END_MODULE