FastQCFastQC Report
Wed 25 May 2016
SRR2088258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621813
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT326961.2470759737631936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT297091.1331471771632835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT268231.0230706766653457No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG185030.7057330175721915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113800.4340507885192422No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA108120.4123863906388442No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA105420.4020881733365423No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT103730.3956422521362126No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT100510.38336067446457855No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA76170.29052415256160524No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT75040.28621415791286414No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG71500.27271205078317945No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT70430.26863090540782275No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA63040.24044430323596688No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA56900.2170253942596211No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA56350.21492760925359666No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT46930.1789982733322323No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA41970.16008006673244812No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.15199405907286295No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC39080.14905716006442868No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38990.14871388615435197No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37360.14249681422740676No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG36790.14032274613025414No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33700.1285370085509531No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31770.12117569025708547No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC29740.11343295650757701No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29300.11175472850275744No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG27580.1051943826657355No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA27490.10485110875565877No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27060.10321102229640328No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA26780.10214305902060902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72450.029.6204281
GTATTGG11900.019.7436981
ATTGGAC12000.019.1166673
ATATCGT500.00703722718.56
CGTCTGT7200.018.24305734
GTATCAA126900.017.4649312
ACGTTTA1401.873559E-917.17857226
TTGGACC19550.017.0332474
CAATGCG1655.4569682E-1116.81818219
TGGACCC20100.016.6592045
GGACCCT20550.016.384436
TATTGGA15850.016.2239742
CGCGAAT700.002593681215.85714335
CTAGGTT1403.4784534E-815.8571434
TATACCG1455.3536496E-815.3103455
TGCGACG1853.0559022E-1015.022
CTTGCGA1508.1132384E-814.810
CGCGCAA1252.9620624E-614.79999921
GTACTAG3150.014.682541
TATACTG3550.014.591555
Produced by FastQC (version 0.11.5)