FastQCFastQC Report
Wed 25 May 2016
SRR2088257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662240
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT225781.358287611897199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200421.2057223986909231No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT167631.008458465684859No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG114150.6867239387814034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76780.4619068245259409No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA72500.4361584368081625No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT70430.4237053614399846No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT67480.4059582250457214No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA62350.37509625565501975No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA52250.31433487342381367No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT45740.27517085378766004No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG45560.27408797766868803No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT44000.26470305130426414No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38630.23239724708826645No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38540.23185580902878042No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35930.21615410530368662No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28930.17404225623255365No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA26770.1610477428048898No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC26690.16056646452979112No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26070.1568365578977765No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG24500.14739147174896525No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23990.14432332274521129No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22190.1334945615554914No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC21870.13156944845509674No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20040.12056020791221483No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA19620.11803349696794685No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC19450.11701078063336222No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16810.10112859755510635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47550.030.6193471
AACCGTG502.7028043E-422.27
GTATTGG5300.018.8490561
GTATCAA80450.018.764452
TTGGACC8700.017.8620684
TGGACCC8900.017.252815
CAATTCG650.00158033517.07692315
GGACCCT9400.016.5319146
ATTGGAC6250.016.283
TATTGGA7050.015.744682
TGGACTA1557.214112E-915.51612955
GACCCTC9450.015.4656097
CGTCTGT4000.015.26250134
GTTATAC1353.9749466E-715.0740753
AGCGGAC908.276754E-414.38888836
TTTTTAC11550.014.2554111
CGTTGAT13450.014.16728634
TAAACGT800.00630018113.8754
AGTATCA3200.013.8751
TTAAACG800.00630018113.87518
Produced by FastQC (version 0.11.5)