##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088257_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1662240
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.87898257772644	31.0	30.0	31.0	27.0	34.0
2	30.648194604870536	31.0	30.0	34.0	27.0	34.0
3	30.682831600731543	31.0	30.0	34.0	27.0	34.0
4	34.82321866878429	35.0	35.0	37.0	32.0	37.0
5	34.688410819135626	35.0	35.0	37.0	32.0	37.0
6	34.79772535855231	35.0	35.0	37.0	32.0	37.0
7	34.44451282606603	35.0	35.0	37.0	30.0	37.0
8	34.65184570218501	35.0	35.0	37.0	30.0	37.0
9	36.027715612667244	37.0	35.0	39.0	30.0	39.0
10	35.92683908460872	37.0	35.0	39.0	30.0	39.0
11	36.04398522475695	37.0	35.0	39.0	30.0	39.0
12	35.76942499278083	37.0	35.0	39.0	30.0	39.0
13	35.94282113292906	37.0	35.0	39.0	30.0	39.0
14	36.7878976561748	38.0	36.0	40.0	31.0	41.0
15	36.83298019539898	38.0	36.0	40.0	31.0	41.0
16	36.72502225912022	38.0	36.0	40.0	31.0	41.0
17	36.83707587352007	38.0	36.0	40.0	31.0	41.0
18	36.84053506112234	38.0	36.0	40.0	31.0	41.0
19	36.91824225141977	39.0	36.0	40.0	31.0	41.0
20	36.898952618153814	39.0	36.0	40.0	31.0	41.0
21	36.83584259794013	39.0	36.0	40.0	31.0	41.0
22	36.74324225141977	39.0	35.0	40.0	30.0	41.0
23	36.60827557994032	38.0	35.0	40.0	30.0	41.0
24	36.53971628645683	38.0	35.0	40.0	30.0	41.0
25	36.45223613918568	38.0	35.0	40.0	30.0	41.0
26	36.17710920204062	38.0	35.0	40.0	30.0	41.0
27	36.01443052748099	38.0	34.0	40.0	29.0	41.0
28	35.859820483203386	38.0	34.0	40.0	29.0	41.0
29	35.669047790932716	38.0	34.0	40.0	27.0	41.0
30	35.50489279526422	38.0	34.0	40.0	27.0	41.0
31	35.2400038502262	38.0	34.0	40.0	27.0	41.0
32	35.028571686399076	37.0	33.0	40.0	26.0	41.0
33	35.17597579170276	37.0	33.0	40.0	27.0	41.0
34	35.157116300895176	38.0	33.0	40.0	27.0	41.0
35	35.061267927615745	38.0	33.0	40.0	26.0	41.0
36	34.9742540186736	38.0	33.0	40.0	25.0	41.0
37	34.819277601309075	38.0	33.0	40.0	25.0	41.0
38	34.69701306670517	37.0	33.0	40.0	25.0	41.0
39	34.65841755703148	38.0	33.0	40.0	24.0	41.0
40	34.501337953604775	38.0	33.0	40.0	24.0	41.0
41	34.337632953123496	38.0	33.0	40.0	23.0	41.0
42	34.12178024833959	37.0	33.0	40.0	22.0	41.0
43	33.777119429203964	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	10.0
16	17.0
17	80.0
18	173.0
19	358.0
20	792.0
21	1661.0
22	2991.0
23	5139.0
24	8390.0
25	12879.0
26	19379.0
27	27587.0
28	37330.0
29	49643.0
30	62867.0
31	76803.0
32	91978.0
33	110132.0
34	130610.0
35	154448.0
36	186232.0
37	228740.0
38	262842.0
39	191156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.88884878236596	25.61098277023775	13.786035710848013	21.714132736548272
2	18.610910578496487	23.754812782750985	35.196602175377805	22.437674463374723
3	20.54691259986524	26.404791125228606	32.47286793724131	20.57542833766484
4	13.46670757532005	16.731037635961112	35.33111945326788	34.47113533545096
5	11.04226826451054	41.01856530946193	34.86012850129946	13.079037924728079
6	31.743129752622966	36.664861873135045	14.923837712965637	16.66817066127635
7	24.180623736644527	35.669337761093466	21.436194532678794	18.713843969583213
8	27.670733468091253	34.119260756569446	20.03338868033497	18.17661709500433
9	24.81013572047358	13.614820964481664	23.79403696217153	37.781006352873234
10	16.037275002406393	28.380979882568102	34.13700789296371	21.444737222061796
11	31.303421888535954	21.70763547983444	25.174042256232553	21.814900375397052
12	19.90440610260853	27.782630667051688	32.04314659736259	20.269816632977186
13	31.278876696505918	20.643408894022524	29.10295745500048	18.974756954471076
14	20.694484550967367	22.7559798825681	28.746029454230438	27.803506112234093
15	23.144912888632206	29.164380594859946	26.831745115025505	20.858961401482336
16	21.60458176917894	28.62619116373087	27.56028010395611	22.208946963134085
17	19.193076812012706	27.436651747040138	28.235633843488305	25.134637597458852
18	20.32522379439792	26.243141784579844	32.72253104244874	20.70910337857349
19	19.561615651169507	24.835462989700645	34.30882423717394	21.294097121955915
20	24.079254499951873	23.747352969486958	32.86691452497834	19.30647800558283
21	23.6246270093368	24.433776109346425	31.67196072769275	20.269636153624027
22	21.688985946674364	24.250168447396284	32.54860910578496	21.512236500144382
23	20.97079844065839	26.363160554432575	33.45623977283665	19.209801232072383
24	19.58423573009914	25.269455674270862	31.88239965347964	23.26390894215035
25	20.834476369236693	26.189659736259507	33.061230628549424	19.91463326595437
26	21.919518240446624	25.355905284435458	31.611139185677157	21.113437289440753
27	20.876588218307827	26.709801232072383	31.335246414476853	21.07836413514294
28	20.647138800654538	26.240675233419964	31.50062566175763	21.61156030416787
29	19.65516411589181	26.13052266820676	32.44038165367215	21.773931562229283
30	19.16546347097892	26.349985561651746	34.256304745403796	20.22824622196554
31	20.58138415631918	25.855833092694198	32.88995572239869	20.67282702858793
32	21.54658773702955	26.259986524208294	30.921888535951485	21.271537202810663
33	22.46931850996246	26.103210126094904	30.442415054384448	20.985056309558185
34	20.822263933006063	28.855640581384158	30.603522956973723	19.71857252863606
35	19.393408894022524	30.009084127442488	30.701763884878236	19.895743093656755
36	20.67571469823852	26.327305322937722	32.76801183944556	20.22896813937819
37	20.613329001828856	26.894251131003948	31.837941572817403	20.654478294349794
38	21.005871594956204	26.451715757050724	32.20750312830879	20.33490951968428
39	21.956937626335545	26.027288478198095	32.007291365867744	20.008482529598616
40	20.189082202329388	26.95711810568871	30.987402541149294	21.86639715083261
41	21.336690249302144	26.406415439407066	30.934341611319667	21.322552699971123
42	18.835126094908077	28.179203965732984	31.19669843103282	21.788971508326114
43	19.531475599191452	27.979653960920203	30.830626143035904	21.65824429685244
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	138.5
2	186.0
3	468.0
4	750.0
5	750.0
6	1556.0
7	2362.0
8	2648.5
9	2935.0
10	4713.5
11	6492.0
12	6492.0
13	12546.0
14	18600.0
15	31120.0
16	43640.0
17	41737.5
18	39835.0
19	39835.0
20	44266.0
21	48697.0
22	40146.0
23	31595.0
24	34320.5
25	37046.0
26	37046.0
27	40701.5
28	44357.0
29	46435.5
30	48514.0
31	52220.5
32	55927.0
33	55927.0
34	60080.5
35	64234.0
36	69404.5
37	74575.0
38	76787.5
39	79000.0
40	79000.0
41	82717.0
42	86434.0
43	97436.5
44	108439.0
45	134823.0
46	161207.0
47	161207.0
48	164380.0
49	167553.0
50	153221.5
51	138890.0
52	129667.0
53	120444.0
54	120444.0
55	103627.5
56	86811.0
57	71430.5
58	56050.0
59	48790.0
60	41530.0
61	41530.0
62	36115.0
63	30700.0
64	26615.5
65	22531.0
66	18765.5
67	15000.0
68	15000.0
69	12760.0
70	10520.0
71	8783.0
72	7046.0
73	5735.5
74	4425.0
75	4425.0
76	3514.5
77	2604.0
78	1997.0
79	1390.0
80	1122.5
81	855.0
82	855.0
83	656.0
84	457.0
85	361.0
86	265.0
87	200.0
88	135.0
89	135.0
90	108.5
91	82.0
92	54.5
93	27.0
94	16.0
95	5.0
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1662240.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.47646031770063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06137075219992	36.58601110544436
2	12.964183769439384	12.30987112562335
3	4.238492883282601	6.036859175400688
4	1.8961013488857446	3.600807217948508
5	0.9901322344481706	2.3503986869027416
6	0.5762388888301436	1.641466964343613
7	0.37046639691058253	1.2311903234376764
8	0.28654632200046193	1.08833640685104
9	0.1958669571822369	0.8369162838190116
>10	1.2400235442094607	11.108635904029548
>50	0.1057959827026749	3.443593378014325
>100	0.06135883713413367	5.48418718652542
>500	0.005624491829319917	1.8286765866802022
>1k	0.006519297347620813	5.7179825648425355
>5k	7.669761585436251E-4	2.438417515472715
>10k+	5.113174390290834E-4	4.2966495746643085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22578	1.358287611897199	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20042	1.2057223986909231	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16763	1.008458465684859	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11415	0.6867239387814034	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7678	0.4619068245259409	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7250	0.4361584368081625	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7043	0.4237053614399846	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6748	0.4059582250457214	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6235	0.37509625565501975	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5225	0.31433487342381367	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4574	0.27517085378766004	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4556	0.27408797766868803	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4400	0.26470305130426414	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3863	0.23239724708826645	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3854	0.23185580902878042	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3593	0.21615410530368662	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2893	0.17404225623255365	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2677	0.1610477428048898	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2669	0.16056646452979112	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2607	0.1568365578977765	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2450	0.14739147174896525	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2399	0.14432332274521129	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2219	0.1334945615554914	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2187	0.13156944845509674	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2004	0.12056020791221483	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1962	0.11803349696794685	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1945	0.11701078063336222	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1681	0.10112859755510635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.015978438733275E-5	0.0
5	0.0	0.0	0.0	6.015978438733275E-5	0.0
6	0.0	0.0	0.0	6.015978438733275E-5	0.0
7	0.0	0.0	0.0	1.203195687746655E-4	0.0
8	0.0	0.0	0.0	1.8047935316199828E-4	0.0
9	0.0	0.0	0.0	2.40639137549331E-4	0.0
10	0.0	0.0	0.0	2.40639137549331E-4	0.0
11	0.0	0.0	0.0	3.007989219366638E-4	0.0
12	0.0	0.0	0.0	3.6095870632399656E-4	0.0
13	6.015978438733275E-5	0.0	0.0	4.2111849071132927E-4	0.0
14	6.015978438733275E-5	0.0	0.0	7.219174126479931E-4	0.0
15	6.015978438733275E-5	0.0	0.0	9.62556550197324E-4	0.0
16	6.015978438733275E-5	0.0	0.0	0.0014438348252959862	0.0
17	6.015978438733275E-5	0.0	0.0	0.002165752237943979	0.0
18	6.015978438733275E-5	0.0	0.0	0.002466551159880643	0.0
19	6.015978438733275E-5	0.0	0.0	0.002947829434979305	0.0
20	6.015978438733275E-5	0.0	0.0	0.0036095870632399654	0.0
21	6.015978438733275E-5	0.0	0.0	0.005113581672923285	0.0
22	6.015978438733275E-5	0.0	0.0	0.008302050245451921	0.0
23	6.015978438733275E-5	0.0	0.0	0.015521224371931852	0.0
24	6.015978438733275E-5	0.0	0.0	0.026349985561651745	0.0
25	6.015978438733275E-5	0.0	0.0	0.031162768312638367	0.0
26	6.015978438733275E-5	0.0	0.0	0.03675762826066031	0.0
27	6.015978438733275E-5	0.0	0.0	0.06924391182982	0.0
28	6.015978438733275E-5	0.0	0.0	0.10479834440273367	0.0
29	6.015978438733275E-5	0.0	0.0	0.15286601212821252	0.0
30	6.015978438733275E-5	0.0	0.0	0.2517686976609876	0.0
31	6.015978438733275E-5	0.0	0.0	0.3701029935508711	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4755	0.0	30.619347	1
AACCGTG	50	2.7028043E-4	22.2	7
GTATTGG	530	0.0	18.849056	1
GTATCAA	8045	0.0	18.76445	2
TTGGACC	870	0.0	17.862068	4
TGGACCC	890	0.0	17.25281	5
CAATTCG	65	0.001580335	17.076923	15
GGACCCT	940	0.0	16.531914	6
ATTGGAC	625	0.0	16.28	3
TATTGGA	705	0.0	15.74468	2
TGGACTA	155	7.214112E-9	15.5161295	5
GACCCTC	945	0.0	15.465609	7
CGTCTGT	400	0.0	15.262501	34
GTTATAC	135	3.9749466E-7	15.074075	3
AGCGGAC	90	8.276754E-4	14.388888	36
TTTTTAC	1155	0.0	14.255411	1
CGTTGAT	1345	0.0	14.167286	34
TAAACGT	80	0.006300181	13.875	4
AGTATCA	320	0.0	13.875	1
TTAAACG	80	0.006300181	13.875	18
>>END_MODULE