FastQCFastQC Report
Wed 25 May 2016
SRR2088256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973373
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT398701.3409013938042755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT362901.2204994126199438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327711.1021489735731103No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG234270.7878930763143407No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA142230.4783456364203213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136340.45853648365004995No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA131420.4419896191967842No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT130000.4372138981553946No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT125080.4206670337021289No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA96430.3243118169163438No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT91920.3091438578341836No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT88210.29666644581759505No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG87730.295052117578252No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA78280.2632700303661868No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA76220.2563418716723398No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA70990.23875242023116508No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT57580.19365212504452015No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49640.16694844541872142No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA49530.16657849519720533No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC48670.1636861571017158No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.15672436656954913No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG46050.15487461546196862No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG45240.1521504365580773No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT40870.13745332321239212No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38810.1305251645185451No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC36530.12285710538166587No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA35220.11845133456179228No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC32930.1107496435865934No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG32530.10940437005380757No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG30570.10281252974315702No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.1024425795216409No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30430.10234168400668198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87650.030.1192261
ACGTTTA1550.023.87096626
GTATTGG11950.023.5313821
ATTGGAC12550.022.7011953
TATTGGA14750.020.4440672
TTGGACC20250.020.0987664
CGCGATA801.6180767E-518.514
CGATACG500.007037445418.49999826
GTATCAA152350.018.0567782
GTCGTCA1653.6379788E-1217.93939424
GGACCCT22000.017.9113646
CGTCTGT8900.017.87640434
TGGACCC22900.017.5305675
CAATGCG1603.45608E-1117.3437519
TTGCGTG1401.873559E-917.1785727
TATACCG2050.017.1463435
ACGTGCG1107.817853E-716.8181829
GACCCTC23400.016.5235047
TGCGTCT9000.016.2388932
TCACGTT2400.016.187524
Produced by FastQC (version 0.11.5)