##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2088256_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2973373 Sequences flagged as poor quality 0 Sequence length 43 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.061325302947193 31.0 30.0 33.0 27.0 34.0 2 30.86795938484677 31.0 31.0 34.0 27.0 34.0 3 30.85025323092663 31.0 31.0 34.0 27.0 34.0 4 35.02250777147704 35.0 35.0 37.0 32.0 37.0 5 34.94061357253194 35.0 35.0 37.0 32.0 37.0 6 35.04446801662623 36.0 35.0 37.0 32.0 37.0 7 34.678437585866284 35.0 35.0 37.0 31.0 37.0 8 34.897373454322754 36.0 35.0 37.0 32.0 37.0 9 36.311615125313914 38.0 35.0 39.0 32.0 39.0 10 36.25744802283467 38.0 35.0 39.0 32.0 39.0 11 36.347672155494784 38.0 35.0 39.0 32.0 39.0 12 36.08060206371686 38.0 35.0 39.0 31.0 39.0 13 36.257914160113785 38.0 35.0 39.0 32.0 39.0 14 37.16093339113525 39.0 36.0 40.0 31.0 41.0 15 37.2032136566788 39.0 36.0 40.0 32.0 41.0 16 37.10963508446468 39.0 36.0 40.0 31.0 41.0 17 37.21713454719606 39.0 36.0 40.0 32.0 41.0 18 37.22343984424423 39.0 36.0 40.0 32.0 41.0 19 37.30823815242824 39.0 36.0 40.0 31.0 41.0 20 37.27780806511662 39.0 36.0 40.0 31.0 41.0 21 37.2072625936941 39.0 36.0 40.0 31.0 41.0 22 37.126610082219756 39.0 36.0 40.0 31.0 41.0 23 37.00847522325655 39.0 36.0 40.0 31.0 41.0 24 36.935947154965085 39.0 35.0 40.0 31.0 41.0 25 36.85578062355446 39.0 35.0 40.0 31.0 41.0 26 36.59616368346656 38.0 35.0 40.0 30.0 41.0 27 36.45032493400593 38.0 35.0 40.0 30.0 41.0 28 36.293794623143484 38.0 35.0 40.0 30.0 41.0 29 36.13071955654403 38.0 35.0 40.0 30.0 41.0 30 35.952633255228996 38.0 34.0 40.0 29.0 41.0 31 35.69163606449645 38.0 34.0 40.0 29.0 41.0 32 35.47553636896548 38.0 34.0 40.0 27.0 41.0 33 35.59378288563191 38.0 34.0 40.0 29.0 41.0 34 35.5836095908586 38.0 34.0 40.0 28.0 41.0 35 35.47815292598675 38.0 34.0 40.0 27.0 41.0 36 35.39139018212649 38.0 34.0 40.0 27.0 41.0 37 35.22828215632549 38.0 34.0 40.0 26.0 41.0 38 35.09862772010104 38.0 34.0 40.0 26.0 41.0 39 35.06230600735259 38.0 33.0 40.0 25.0 41.0 40 34.88676799042703 38.0 33.0 40.0 24.0 41.0 41 34.716203449752186 38.0 33.0 40.0 24.0 41.0 42 34.497218478811774 38.0 33.0 40.0 23.0 41.0 43 34.16157609556554 38.0 33.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 5.0 14 4.0 15 9.0 16 33.0 17 87.0 18 206.0 19 492.0 20 1057.0 21 2243.0 22 4037.0 23 6964.0 24 11494.0 25 18414.0 26 28202.0 27 40881.0 28 57620.0 29 77869.0 30 100623.0 31 123976.0 32 151837.0 33 185354.0 34 223470.0 35 267304.0 36 326237.0 37 411370.0 38 478959.0 39 454624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.65536782637093 24.96208851025418 13.4541142332294 21.928429430145492 2 17.938650818447602 22.752375837138494 36.28074916937767 23.02822417503623 3 19.799063218775444 26.52842411631504 32.30620578043858 21.366306884470937 4 12.701030109575893 15.651114071460256 35.35271222278537 36.295143596178484 5 10.974842375981755 40.657630240134694 34.89804340054208 13.46948398334148 6 31.74966611992508 35.77835676855881 15.194225547887871 17.277751563628243 7 24.4283512361214 35.38136654903371 21.21005336363786 18.98022885120703 8 28.060455247289863 33.322425407104994 19.996246686843527 18.620872658761613 9 25.044721937005548 13.48720796213593 23.796644416963495 37.67142568389502 10 16.32475979300276 27.65172751619121 33.78348427862902 22.24002841217701 11 31.229314317443524 21.435756630601006 25.424156336927794 21.91077271502768 12 20.39562476688932 27.22615023409441 31.728713484651944 20.649511514364328 13 31.55534135811417 20.40396546279259 29.01751647035202 19.023176708741218 14 21.064562031067073 22.321518356425514 28.269376226931502 28.34454338557591 15 23.13820028634147 28.69794674263875 27.021466866081045 21.142386104938733 16 21.947229627766177 28.169254244253917 27.15404357273709 22.729472555242815 17 19.295090121555553 26.806895737601703 27.744517758115112 26.15349638272763 18 20.605621965357187 26.034069724854568 32.59274904292196 20.767559266866282 19 19.588696070086062 24.38668138844336 34.0640410738915 21.960581467579075 20 24.729726139303747 23.10016267720195 32.558276408644325 19.611834774849974 21 23.98387286088896 24.160271852875507 31.27602894086951 20.57982634536602 22 22.051017480820605 23.74000840123321 32.30721473558817 21.901759382358016 23 21.113227301115604 26.052230917547174 33.431426195098965 19.403115586238258 24 19.495401350587365 24.92398363743802 31.606091802138515 23.9745232098361 25 20.905416172138512 25.958936198048477 32.98842762075259 20.14722000906042 26 22.451269988662705 24.83499379324424 31.439782361647868 21.27395385644519 27 20.94436184091266 26.492438049313023 30.957770854850704 21.605429254923617 28 20.708333599585387 25.807424766418475 31.152970044457927 22.33127158953821 29 19.672035765442143 25.864565259723555 32.13434708662519 22.329051888209115 30 18.969365767429785 26.1450547913094 34.27091051139564 20.614668929865175 31 20.647930817963303 25.496969266889824 33.00594980851713 20.849150106629743 32 21.92308196785267 25.66586835893109 30.539558945345906 21.871490727870334 33 22.974480497401437 25.628402491043 30.040731519388924 21.35638549216664 34 20.828836476284675 28.799683053555675 30.230347823835086 20.14113264632456 35 19.39484215401162 30.16826345029702 30.263744239286495 20.173150156404866 36 20.69736962029318 25.949317492289058 32.753273807221625 20.60003908019613 37 20.74996981542511 26.404860742328662 31.579589913542634 21.265579528703597 38 21.008733179456463 25.979384355746827 32.291239612386335 20.720642852410375 39 22.142159762666843 25.279539432153314 32.048485003395136 20.529815801784707 40 20.119002896710235 26.392719648695266 30.98645881293736 22.501818641657135 41 21.594297116439815 25.72472407599047 30.81483554199221 21.866143265577513 42 18.684403201347425 27.725885719686026 31.333539384396104 22.256171694570444 43 19.465267223452962 27.52584354536077 30.84698757942579 22.161901651760477 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 162.0 1 276.5 2 391.0 3 1056.5 4 1722.0 5 1722.0 6 3237.5 7 4753.0 8 5260.5 9 5768.0 10 9040.0 11 12312.0 12 12312.0 13 23046.0 14 33780.0 15 55972.5 16 78165.0 17 75135.0 18 72105.0 19 72105.0 20 77276.5 21 82448.0 22 65277.5 23 48107.0 24 52588.5 25 57070.0 26 57070.0 27 62591.0 28 68112.0 29 72004.0 30 75896.0 31 81483.5 32 87071.0 33 87071.0 34 94517.0 35 101963.0 36 111826.5 37 121690.0 38 127633.5 39 133577.0 40 133577.0 41 141679.5 42 149782.0 43 173471.0 44 197160.0 45 252371.0 46 307582.0 47 307582.0 48 315611.5 49 323641.0 50 294639.0 51 265637.0 52 248163.0 53 230689.0 54 230689.0 55 197356.0 56 164023.0 57 131586.5 58 99150.0 59 85830.5 60 72511.0 61 72511.0 62 63699.0 63 54887.0 64 48234.0 65 41581.0 66 35389.0 67 29197.0 68 29197.0 69 24847.0 70 20497.0 71 17044.0 72 13591.0 73 10873.5 74 8156.0 75 8156.0 76 6392.0 77 4628.0 78 3646.5 79 2665.0 80 2019.5 81 1374.0 82 1374.0 83 1019.0 84 664.0 85 525.0 86 386.0 87 321.5 88 257.0 89 257.0 90 207.5 91 158.0 92 100.0 93 42.0 94 29.5 95 17.0 96 17.0 97 10.5 98 4.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 2973373.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.87259141170125 #Duplication Level Percentage of deduplicated Percentage of total 1 71.42391406340002 27.05008714347612 2 14.415734654549773 10.919224569425316 3 5.51146228313838 6.26200077390906 4 2.761011932673099 4.182667072358395 5 1.5290284767576248 2.8954135378548735 6 0.9445524418572833 2.1463589218431354 7 0.6112385282809232 1.6204430925671105 8 0.43993090103808796 1.3329058611517668 9 0.2930980461591827 0.9990344291179213 >10 1.7548496892482037 12.747620178747187 >50 0.18846760055711306 4.967059140282033 >100 0.10984902829525466 7.595485086300603 >500 0.007664706384184257 1.9947516170090736 >1k 0.0076647063841831925 6.284748250753713 >5k 7.213841302760653E-4 2.2107899448499553 >10k+ 8.115571465605733E-4 6.7914103803538435 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 39870 1.3409013938042755 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 36290 1.2204994126199438 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 32771 1.1021489735731103 No Hit GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG 23427 0.7878930763143407 No Hit ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA 14223 0.4783456364203213 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13634 0.45853648365004995 No Hit ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA 13142 0.4419896191967842 No Hit ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT 13000 0.4372138981553946 No Hit GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT 12508 0.4206670337021289 No Hit TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA 9643 0.3243118169163438 No Hit GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT 9192 0.3091438578341836 No Hit CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT 8821 0.29666644581759505 No Hit GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG 8773 0.295052117578252 No Hit CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA 7828 0.2632700303661868 No Hit GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA 7622 0.2563418716723398 No Hit CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA 7099 0.23875242023116508 No Hit TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT 5758 0.19365212504452015 No Hit GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC 4964 0.16694844541872142 No Hit GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA 4953 0.16657849519720533 No Hit AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC 4867 0.1636861571017158 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4660 0.15672436656954913 No Hit ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG 4605 0.15487461546196862 No Hit CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG 4524 0.1521504365580773 No Hit TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT 4087 0.13745332321239212 No Hit TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA 3881 0.1305251645185451 No Hit CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC 3653 0.12285710538166587 No Hit CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA 3522 0.11845133456179228 No Hit GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC 3293 0.1107496435865934 No Hit CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG 3253 0.10940437005380757 No Hit CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG 3057 0.10281252974315702 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3046 0.1024425795216409 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3043 0.10234168400668198 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.3631838319645734E-5 0.0 5 0.0 0.0 0.0 3.3631838319645734E-5 0.0 6 0.0 0.0 0.0 3.3631838319645734E-5 0.0 7 0.0 0.0 0.0 3.3631838319645734E-5 0.0 8 0.0 0.0 0.0 3.3631838319645734E-5 0.0 9 0.0 0.0 0.0 3.3631838319645734E-5 0.0 10 0.0 0.0 0.0 1.3452735327858294E-4 0.0 11 0.0 0.0 0.0 1.3452735327858294E-4 0.0 12 0.0 0.0 0.0 3.3631838319645737E-4 0.0 13 0.0 0.0 0.0 4.035820598357488E-4 0.0 14 0.0 0.0 0.0 4.708457364750403E-4 0.0 15 0.0 0.0 0.0 9.753233112697263E-4 0.0 16 0.0 0.0 0.0 0.001917014784219807 0.0 17 0.0 0.0 0.0 0.002959601772128825 0.0 18 0.0 0.0 0.0 0.0030941291254074077 0.0 19 0.0 0.0 0.0 0.0036658703768413852 0.0 20 0.0 0.0 0.0 0.0051120394245861515 0.0 21 0.0 0.0 0.0 0.007432636268641707 0.0 22 0.0 0.0 0.0 0.012208357310031403 0.0 23 0.0 0.0 0.0 0.022230645129285832 0.0 24 0.0 0.0 0.0 0.03682686296001208 0.0 25 0.0 0.0 0.0 0.04224158892947504 0.0 26 0.0 0.0 0.0 0.049001588431723836 0.0 27 0.0 0.0 0.0 0.09968476877942996 0.0 28 0.0 0.0 0.0 0.15484098362364895 0.0 29 0.0 0.0 0.0 0.22069212305351532 0.0 30 0.0 0.0 0.0 0.3458361934409171 0.0 31 0.0 0.0 0.0 0.5125828478297206 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8765 0.0 30.119226 1 ACGTTTA 155 0.0 23.870966 26 GTATTGG 1195 0.0 23.531382 1 ATTGGAC 1255 0.0 22.701195 3 TATTGGA 1475 0.0 20.444067 2 TTGGACC 2025 0.0 20.098766 4 CGCGATA 80 1.6180767E-5 18.5 14 CGATACG 50 0.0070374454 18.499998 26 GTATCAA 15235 0.0 18.056778 2 GTCGTCA 165 3.6379788E-12 17.939394 24 GGACCCT 2200 0.0 17.911364 6 CGTCTGT 890 0.0 17.876404 34 TGGACCC 2290 0.0 17.530567 5 CAATGCG 160 3.45608E-11 17.34375 19 TTGCGTG 140 1.873559E-9 17.178572 7 TATACCG 205 0.0 17.146343 5 ACGTGCG 110 7.817853E-7 16.818182 9 GACCCTC 2340 0.0 16.523504 7 TGCGTCT 900 0.0 16.23889 32 TCACGTT 240 0.0 16.1875 24 >>END_MODULE